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UniProtKB - Q9CQV6 (MLP3B_MOUSE)

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Protein

Microtubule-associated proteins 1A/1B light chain 3B

Gene

Map1lc3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.By similarity

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • autophagy Source: CAFA
  • cellular response to nitrogen starvation Source: GO_Central
  • mitophagy Source: MGI
  • positive regulation of mucus secretion Source: MGI
Complete GO annotation...

Keywordsi

Biological processAutophagy, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.
R-MMU-5205685. Pink/Parkin Mediated Mitophagy.
R-MMU-8854214. TBC/RABGAPs.
R-MMU-8934903. Receptor Mediated Mitophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3B
Alternative name(s):
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B
Short name:
MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta
Gene namesi
Name:Map1lc3b
Synonyms:Map1alc3, Map1lc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1914693. Map1lc3b.

Subcellular locationi

GO - Cellular componenti

  • autolysosome Source: MGI
  • autophagosome Source: CAFA
  • autophagosome membrane Source: GO_Central
  • axoneme Source: UniProtKB
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: GO_Central
  • endomembrane system Source: UniProtKB-SubCell
  • intracellular Source: MGI
  • membrane Source: MGI
  • microtubule Source: UniProtKB-KW
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000172002 – 120Microtubule-associated proteins 1A/1B light chain 3BAdd BLAST119
PropeptideiPRO_0000017201121 – 125Removed in mature form5

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine; by PKABy similarity1
Lipidationi120Phosphatidylethanolamine amidated glycineBy similarity1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.By similarity
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120 – 121Cleavage; by ATG4B2

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9CQV6.
PaxDbiQ9CQV6.
PRIDEiQ9CQV6.

PTM databases

iPTMnetiQ9CQV6.
PhosphoSitePlusiQ9CQV6.
SwissPalmiQ9CQV6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031812.
ExpressionAtlasiQ9CQV6. baseline and differential.
GenevisibleiQ9CQV6. MM.

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Interacts with FYCO1 (via C-terminus). Interacts with TP53INP1 and TP53INP2 (By similarity). Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG4B, MAPK15 and BNIP3. Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2. Interacts with TBC1D5. Found in a complex with UBQLN1 and UBQLN2. Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4. Interacts with ATG13. Interacts with RETREG1, RETREG2 and RETREG3 (By similarity).By similarity1 Publication

GO - Molecular functioni

Complete GO annotation...

Protein-protein interaction databases

BioGridi212190. 5 interactors.
DIPiDIP-61580N.
IntActiQ9CQV6. 4 interactors.
STRINGi10090.ENSMUSP00000034270.

Structurei

Secondary structure

1125
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Helixi13 – 26Combined sources14
Beta strandi30 – 37Combined sources8
Beta strandi51 – 55Combined sources5
Helixi60 – 70Combined sources11
Beta strandi80 – 83Combined sources4
Turni84 – 86Combined sources3
Helixi95 – 102Combined sources8
Beta strandi109 – 115Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WRDX-ray1.90A/B1-125[»]
ProteinModelPortaliQ9CQV6.
SMRiQ9CQV6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ9CQV6.
KOiK10435.
PhylomeDBiQ9CQV6.
TreeFamiTF312964.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiView protein in InterPro
IPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiView protein in Pfam
PF02991. Atg8. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT 
        60         70         80         90        100
KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE 
       110        120 
SERDEDGFLY MVYASQETFG TAMAV
Length:125
Mass (Da):14,617
Last modified:January 23, 2007 - v3
Checksum:i520E29028163FA8D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89V → L in BAB22641 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF255953 mRNA. Translation: AAL83723.1.
AK002795 mRNA. Translation: BAB22364.1.
AK003106 mRNA. Translation: BAB22569.1.
AK003205 mRNA. Translation: BAB22641.1.
AK003558 mRNA. Translation: BAB22855.1.
AK012604 mRNA. Translation: BAB28350.1.
AK132329 mRNA. Translation: BAE21108.1.
AK151614 mRNA. Translation: BAE30551.1.
BC068180 mRNA. Translation: AAH68180.1.
CCDSiCCDS22726.1.
RefSeqiNP_080436.1. NM_026160.4.
UniGeneiMm.28357.

Genome annotation databases

EnsembliENSMUST00000034270; ENSMUSP00000034270; ENSMUSG00000031812.
GeneIDi67443.
KEGGimmu:67443.
UCSCiuc009nrw.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMLP3B_MOUSE
AccessioniPrimary (citable) accession number: Q9CQV6
Secondary accession number(s): Q3U9W5, Q9D1R0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families