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Q9CQV6 (MLP3B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microtubule-associated proteins 1A/1B light chain 3B
Alternative name(s):
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B
Short name=MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta
Gene names
Name:Map1lc3b
Synonyms:Map1alc3, Map1lc3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length125 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation By similarity.

Subunit structure

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts with CABP1 By similarity. Interacts with FYCO1 (via C-terminus) By similarity. Interacts with TP53INP1 and TP53INP2 By similarity. Interacts with TBC1D25. Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy By similarity. Interacts with ATG4B, MAPK15 and BNIP3 By similarity. Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2 By similarity. Ref.9

Subcellular location

Cytoplasmcytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes By similarity. Localizes also to discrete punctae along the ciliary axoneme. Ref.5 Ref.6 Ref.7 Ref.10

Post-translational modification

The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II By similarity. Ref.6 Ref.7

Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) By similarity. Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity By similarity.

Sequence similarities

Belongs to the ATG8 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 120119Microtubule-associated proteins 1A/1B light chain 3B
PRO_0000017200
Propeptide121 – 1255Removed in mature form
PRO_0000017201

Sites

Site120 – 1212Cleavage; by ATG4B

Amino acid modifications

Modified residue121Phosphoserine; by PKA By similarity
Lipidation1201Phosphatidylethanolamine amidated glycine By similarity

Experimental info

Sequence conflict891V → L in BAB22641. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9CQV6 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 520E29028163FA8D

FASTA12514,617
        10         20         30         40         50         60 
MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 

        70         80         90        100        110        120 
SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SERDEDGFLY MVYASQETFG 


TAMAV 

« Hide

References

« Hide 'large scale' references
[1]Yu L.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Bone marrow, Embryo, Head, Heart and Kidney.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 31-37 AND 52-65, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[5]"Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells."
Mizushima N., Yamamoto A., Hatano M., Kobayashi Y., Kabeya Y., Suzuki K., Tokuhisa T., Ohsumi Y., Yoshimori T.
J. Cell Biol. 152:657-668(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing."
Tanida I., Nishitani T., Nemoto T., Ueno T., Kominami E.
Biochem. Biophys. Res. Commun. 296:1164-1170(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PROTEOLYTIC CLEAVAGE.
[7]"A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L."
Hemelaar J., Lelyveld V.S., Kessler B.M., Ploegh H.L.
J. Biol. Chem. 278:51841-51850(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE BY ATG4B, SUBCELLULAR LOCATION.
[8]"LC3 conjugation system in mammalian autophagy."
Tanida I., Ueno T., Kominami E.
Int. J. Biochem. Cell Biol. 36:2503-2518(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[9]"OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation."
Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M.
J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TBC1D25.
[10]"Functional interaction between autophagy and ciliogenesis."
Pampliega O., Orhon I., Patel B., Sridhar S., Diaz-Carretero A., Beau I., Codogno P., Satir B.H., Satir P., Cuervo A.M.
Nature 502:194-200(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF255953 mRNA. Translation: AAL83723.1.
AK002795 mRNA. Translation: BAB22364.1.
AK003106 mRNA. Translation: BAB22569.1.
AK003205 mRNA. Translation: BAB22641.1.
AK003558 mRNA. Translation: BAB22855.1.
AK012604 mRNA. Translation: BAB28350.1.
AK132329 mRNA. Translation: BAE21108.1.
AK151614 mRNA. Translation: BAE30551.1.
BC068180 mRNA. Translation: AAH68180.1.
CCDSCCDS22726.1.
RefSeqNP_080436.1. NM_026160.4.
UniGeneMm.28357.

3D structure databases

ProteinModelPortalQ9CQV6.
SMRQ9CQV6. Positions 5-122.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid212190. 5 interactions.
IntActQ9CQV6. 3 interactions.
STRING10090.ENSMUSP00000034270.

PTM databases

PhosphoSiteQ9CQV6.

Proteomic databases

MaxQBQ9CQV6.
PaxDbQ9CQV6.
PRIDEQ9CQV6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034270; ENSMUSP00000034270; ENSMUSG00000031812.
GeneID67443.
KEGGmmu:67443.
UCSCuc009nrw.1. mouse.

Organism-specific databases

CTD81631.
MGIMGI:1914693. Map1lc3b.

Phylogenomic databases

eggNOGNOG283348.
GeneTreeENSGT00390000012937.
HOGENOMHOG000232034.
HOVERGENHBG051706.
InParanoidQ9CQV6.
KOK10435.
OrthoDBEOG7XH6RV.
PhylomeDBQ9CQV6.
TreeFamTF312964.

Gene expression databases

ArrayExpressQ9CQV6.
BgeeQ9CQV6.
GenevestigatorQ9CQV6.

Family and domain databases

InterProIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERPTHR10969. PTHR10969. 1 hit.
PfamPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMSSF54236. SSF54236. 1 hit.
ProtoNetSearch...

Other

NextBio324582.
PROQ9CQV6.
SOURCESearch...

Entry information

Entry nameMLP3B_MOUSE
AccessionPrimary (citable) accession number: Q9CQV6
Secondary accession number(s): Q3U9W5, Q9D1R0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot