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Q9CQV6

- MLP3B_MOUSE

UniProt

Q9CQV6 - MLP3B_MOUSE

Protein

Microtubule-associated proteins 1A/1B light chain 3B

Gene

Map1lc3b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei120 – 1212Cleavage; by ATG4B

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. positive regulation of mucus secretion Source: MGI

    Keywords - Biological processi

    Autophagy, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microtubule-associated proteins 1A/1B light chain 3B
    Alternative name(s):
    Autophagy-related protein LC3 B
    Autophagy-related ubiquitin-like modifier LC3 B
    MAP1 light chain 3-like protein 2
    MAP1A/MAP1B light chain 3 B
    Short name:
    MAP1A/MAP1B LC3 B
    Microtubule-associated protein 1 light chain 3 beta
    Gene namesi
    Name:Map1lc3b
    Synonyms:Map1alc3, Map1lc3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1914693. Map1lc3b.

    Subcellular locationi

    Cytoplasmcytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane; Lipid-anchor
    Note: LC3-II binds to the autophagic membranes By similarity. Localizes also to discrete punctae along the ciliary axoneme.By similarity

    GO - Cellular componenti

    1. autophagic vacuole Source: MGI
    2. autophagic vacuole membrane Source: UniProtKB-SubCell
    3. axoneme Source: UniProtKB
    4. cytoplasmic vesicle Source: UniProtKB-KW
    5. endomembrane system Source: UniProtKB-SubCell
    6. membrane Source: MGI
    7. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 120119Microtubule-associated proteins 1A/1B light chain 3BPRO_0000017200Add
    BLAST
    Propeptidei121 – 1255Removed in mature formPRO_0000017201

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei12 – 121Phosphoserine; by PKABy similarity
    Lipidationi120 – 1201Phosphatidylethanolamine amidated glycineBy similarity

    Post-translational modificationi

    The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II By similarity.By similarity
    Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.By similarity

    Keywords - PTMi

    Lipoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ9CQV6.
    PaxDbiQ9CQV6.
    PRIDEiQ9CQV6.

    PTM databases

    PhosphoSiteiQ9CQV6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9CQV6.
    BgeeiQ9CQV6.
    GenevestigatoriQ9CQV6.

    Interactioni

    Subunit structurei

    3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts with CABP1 By similarity. Interacts with FYCO1 (via C-terminus) By similarity. Interacts with TP53INP1 and TP53INP2 By similarity. Interacts with TBC1D25. Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy By similarity. Interacts with ATG4B, MAPK15 and BNIP3 By similarity. Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi212190. 5 interactions.
    IntActiQ9CQV6. 3 interactions.
    STRINGi10090.ENSMUSP00000034270.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CQV6.
    SMRiQ9CQV6. Positions 5-122.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATG8 family.Curated

    Phylogenomic databases

    eggNOGiNOG283348.
    GeneTreeiENSGT00390000012937.
    HOGENOMiHOG000232034.
    HOVERGENiHBG051706.
    InParanoidiQ9CQV6.
    KOiK10435.
    OrthoDBiEOG7XH6RV.
    PhylomeDBiQ9CQV6.
    TreeFamiTF312964.

    Family and domain databases

    InterProiIPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR10969. PTHR10969. 1 hit.
    PfamiPF02991. Atg8. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9CQV6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT    50
    KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE 100
    SERDEDGFLY MVYASQETFG TAMAV 125
    Length:125
    Mass (Da):14,617
    Last modified:January 23, 2007 - v3
    Checksum:i520E29028163FA8D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti89 – 891V → L in BAB22641. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF255953 mRNA. Translation: AAL83723.1.
    AK002795 mRNA. Translation: BAB22364.1.
    AK003106 mRNA. Translation: BAB22569.1.
    AK003205 mRNA. Translation: BAB22641.1.
    AK003558 mRNA. Translation: BAB22855.1.
    AK012604 mRNA. Translation: BAB28350.1.
    AK132329 mRNA. Translation: BAE21108.1.
    AK151614 mRNA. Translation: BAE30551.1.
    BC068180 mRNA. Translation: AAH68180.1.
    CCDSiCCDS22726.1.
    RefSeqiNP_080436.1. NM_026160.4.
    UniGeneiMm.28357.

    Genome annotation databases

    EnsembliENSMUST00000034270; ENSMUSP00000034270; ENSMUSG00000031812.
    GeneIDi67443.
    KEGGimmu:67443.
    UCSCiuc009nrw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF255953 mRNA. Translation: AAL83723.1 .
    AK002795 mRNA. Translation: BAB22364.1 .
    AK003106 mRNA. Translation: BAB22569.1 .
    AK003205 mRNA. Translation: BAB22641.1 .
    AK003558 mRNA. Translation: BAB22855.1 .
    AK012604 mRNA. Translation: BAB28350.1 .
    AK132329 mRNA. Translation: BAE21108.1 .
    AK151614 mRNA. Translation: BAE30551.1 .
    BC068180 mRNA. Translation: AAH68180.1 .
    CCDSi CCDS22726.1.
    RefSeqi NP_080436.1. NM_026160.4.
    UniGenei Mm.28357.

    3D structure databases

    ProteinModelPortali Q9CQV6.
    SMRi Q9CQV6. Positions 5-122.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 212190. 5 interactions.
    IntActi Q9CQV6. 3 interactions.
    STRINGi 10090.ENSMUSP00000034270.

    PTM databases

    PhosphoSitei Q9CQV6.

    Proteomic databases

    MaxQBi Q9CQV6.
    PaxDbi Q9CQV6.
    PRIDEi Q9CQV6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034270 ; ENSMUSP00000034270 ; ENSMUSG00000031812 .
    GeneIDi 67443.
    KEGGi mmu:67443.
    UCSCi uc009nrw.1. mouse.

    Organism-specific databases

    CTDi 81631.
    MGIi MGI:1914693. Map1lc3b.

    Phylogenomic databases

    eggNOGi NOG283348.
    GeneTreei ENSGT00390000012937.
    HOGENOMi HOG000232034.
    HOVERGENi HBG051706.
    InParanoidi Q9CQV6.
    KOi K10435.
    OrthoDBi EOG7XH6RV.
    PhylomeDBi Q9CQV6.
    TreeFami TF312964.

    Miscellaneous databases

    NextBioi 324582.
    PROi Q9CQV6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9CQV6.
    Bgeei Q9CQV6.
    Genevestigatori Q9CQV6.

    Family and domain databases

    InterProi IPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR10969. PTHR10969. 1 hit.
    Pfami PF02991. Atg8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Yu L.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Bone marrow, Embryo, Head, Heart and Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 31-37 AND 52-65, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    5. "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells."
      Mizushima N., Yamamoto A., Hatano M., Kobayashi Y., Kabeya Y., Suzuki K., Tokuhisa T., Ohsumi Y., Yoshimori T.
      J. Cell Biol. 152:657-668(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing."
      Tanida I., Nishitani T., Nemoto T., Ueno T., Kominami E.
      Biochem. Biophys. Res. Commun. 296:1164-1170(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PROTEOLYTIC CLEAVAGE.
    7. "A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L."
      Hemelaar J., Lelyveld V.S., Kessler B.M., Ploegh H.L.
      J. Biol. Chem. 278:51841-51850(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE BY ATG4B, SUBCELLULAR LOCATION.
    8. Cited for: REVIEW.
    9. "OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation."
      Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M.
      J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TBC1D25.
    10. Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiMLP3B_MOUSE
    AccessioniPrimary (citable) accession number: Q9CQV6
    Secondary accession number(s): Q3U9W5, Q9D1R0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3