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Q9CQV6

- MLP3B_MOUSE

UniProt

Q9CQV6 - MLP3B_MOUSE

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Protein
Microtubule-associated proteins 1A/1B light chain 3B
Gene
Map1lc3b, Map1alc3, Map1lc3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei120 – 1212Cleavage; by ATG4B

GO - Molecular functioni

  1. protein binding Source: UniProtKB

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Autophagy, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3B
Alternative name(s):
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B
Short name:
MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta
Gene namesi
Name:Map1lc3b
Synonyms:Map1alc3, Map1lc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1914693. Map1lc3b.

Subcellular locationi

Cytoplasmcytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane; Lipid-anchor
Note: LC3-II binds to the autophagic membranes By similarity. Localizes also to discrete punctae along the ciliary axoneme.4 Publications

GO - Cellular componenti

  1. autophagic vacuole Source: MGI
  2. autophagic vacuole membrane Source: UniProtKB-SubCell
  3. axoneme Source: UniProtKB
  4. cytoplasmic vesicle Source: UniProtKB-KW
  5. endomembrane system Source: UniProtKB-SubCell
  6. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 120119Microtubule-associated proteins 1A/1B light chain 3B
PRO_0000017200Add
BLAST
Propeptidei121 – 1255Removed in mature form
PRO_0000017201

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine; by PKA By similarity
Lipidationi120 – 1201Phosphatidylethanolamine amidated glycine By similarity

Post-translational modificationi

The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II By similarity.2 Publications
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) By similarity. Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity By similarity.

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9CQV6.
PaxDbiQ9CQV6.
PRIDEiQ9CQV6.

PTM databases

PhosphoSiteiQ9CQV6.

Expressioni

Gene expression databases

ArrayExpressiQ9CQV6.
BgeeiQ9CQV6.
GenevestigatoriQ9CQV6.

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts with CABP1 By similarity. Interacts with FYCO1 (via C-terminus) By similarity. Interacts with TP53INP1 and TP53INP2 By similarity. Interacts with TBC1D25. Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy By similarity. Interacts with ATG4B, MAPK15 and BNIP3 By similarity. Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2 By similarity.1 Publication

Protein-protein interaction databases

BioGridi212190. 5 interactions.
IntActiQ9CQV6. 3 interactions.
STRINGi10090.ENSMUSP00000034270.

Structurei

3D structure databases

ProteinModelPortaliQ9CQV6.
SMRiQ9CQV6. Positions 5-122.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG8 family.

Phylogenomic databases

eggNOGiNOG283348.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ9CQV6.
KOiK10435.
OrthoDBiEOG7XH6RV.
PhylomeDBiQ9CQV6.
TreeFamiTF312964.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQV6-1 [UniParc]FASTAAdd to Basket

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MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT    50
KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE 100
SERDEDGFLY MVYASQETFG TAMAV 125
Length:125
Mass (Da):14,617
Last modified:January 23, 2007 - v3
Checksum:i520E29028163FA8D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891V → L in BAB22641. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF255953 mRNA. Translation: AAL83723.1.
AK002795 mRNA. Translation: BAB22364.1.
AK003106 mRNA. Translation: BAB22569.1.
AK003205 mRNA. Translation: BAB22641.1.
AK003558 mRNA. Translation: BAB22855.1.
AK012604 mRNA. Translation: BAB28350.1.
AK132329 mRNA. Translation: BAE21108.1.
AK151614 mRNA. Translation: BAE30551.1.
BC068180 mRNA. Translation: AAH68180.1.
CCDSiCCDS22726.1.
RefSeqiNP_080436.1. NM_026160.4.
UniGeneiMm.28357.

Genome annotation databases

EnsembliENSMUST00000034270; ENSMUSP00000034270; ENSMUSG00000031812.
GeneIDi67443.
KEGGimmu:67443.
UCSCiuc009nrw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF255953 mRNA. Translation: AAL83723.1 .
AK002795 mRNA. Translation: BAB22364.1 .
AK003106 mRNA. Translation: BAB22569.1 .
AK003205 mRNA. Translation: BAB22641.1 .
AK003558 mRNA. Translation: BAB22855.1 .
AK012604 mRNA. Translation: BAB28350.1 .
AK132329 mRNA. Translation: BAE21108.1 .
AK151614 mRNA. Translation: BAE30551.1 .
BC068180 mRNA. Translation: AAH68180.1 .
CCDSi CCDS22726.1.
RefSeqi NP_080436.1. NM_026160.4.
UniGenei Mm.28357.

3D structure databases

ProteinModelPortali Q9CQV6.
SMRi Q9CQV6. Positions 5-122.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 212190. 5 interactions.
IntActi Q9CQV6. 3 interactions.
STRINGi 10090.ENSMUSP00000034270.

PTM databases

PhosphoSitei Q9CQV6.

Proteomic databases

MaxQBi Q9CQV6.
PaxDbi Q9CQV6.
PRIDEi Q9CQV6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034270 ; ENSMUSP00000034270 ; ENSMUSG00000031812 .
GeneIDi 67443.
KEGGi mmu:67443.
UCSCi uc009nrw.1. mouse.

Organism-specific databases

CTDi 81631.
MGIi MGI:1914693. Map1lc3b.

Phylogenomic databases

eggNOGi NOG283348.
GeneTreei ENSGT00390000012937.
HOGENOMi HOG000232034.
HOVERGENi HBG051706.
InParanoidi Q9CQV6.
KOi K10435.
OrthoDBi EOG7XH6RV.
PhylomeDBi Q9CQV6.
TreeFami TF312964.

Miscellaneous databases

NextBioi 324582.
PROi Q9CQV6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9CQV6.
Bgeei Q9CQV6.
Genevestigatori Q9CQV6.

Family and domain databases

InterProi IPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
PANTHERi PTHR10969. PTHR10969. 1 hit.
Pfami PF02991. Atg8. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Yu L.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow, Embryo, Head, Heart and Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 31-37 AND 52-65, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells."
    Mizushima N., Yamamoto A., Hatano M., Kobayashi Y., Kabeya Y., Suzuki K., Tokuhisa T., Ohsumi Y., Yoshimori T.
    J. Cell Biol. 152:657-668(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing."
    Tanida I., Nishitani T., Nemoto T., Ueno T., Kominami E.
    Biochem. Biophys. Res. Commun. 296:1164-1170(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PROTEOLYTIC CLEAVAGE.
  7. "A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L."
    Hemelaar J., Lelyveld V.S., Kessler B.M., Ploegh H.L.
    J. Biol. Chem. 278:51841-51850(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE BY ATG4B, SUBCELLULAR LOCATION.
  8. Cited for: REVIEW.
  9. "OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation."
    Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M.
    J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TBC1D25.
  10. Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMLP3B_MOUSE
AccessioniPrimary (citable) accession number: Q9CQV6
Secondary accession number(s): Q3U9W5, Q9D1R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi