UniProtKB - Q9CQV6 (MLP3B_MOUSE)
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Protein
Microtubule-associated proteins 1A/1B light chain 3B
Gene
Map1lc3b
Organism
Mus musculus (Mouse)
Status
Functioni
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.By similarity
GO - Molecular functioni
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- autophagosome assembly Source: GO_Central
- autophagosome maturation Source: MGI
- autophagy Source: CAFA
- autophagy of mitochondrion Source: MGI
- cellular response to nitrogen starvation Source: GO_Central
- cellular response to starvation Source: MGI
- macroautophagy Source: MGI
- positive regulation of mucus secretion Source: MGI
Keywordsi
Biological process | Autophagy, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-1632852. Macroautophagy. R-MMU-5205685. Pink/Parkin Mediated Mitophagy. R-MMU-8854214. TBC/RABGAPs. R-MMU-8934903. Receptor Mediated Mitophagy. |
Names & Taxonomyi
Protein namesi | Recommended name: Microtubule-associated proteins 1A/1B light chain 3BAlternative name(s): Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B MAP1 light chain 3-like protein 2 MAP1A/MAP1B light chain 3 B Short name: MAP1A/MAP1B LC3 B Microtubule-associated protein 1 light chain 3 beta |
Gene namesi | Name:Map1lc3b Synonyms:Map1alc3, Map1lc3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914693. Map1lc3b. |
Subcellular locationi
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000017200 | 1 – 120 | Microtubule-associated proteins 1A/1B light chain 3BAdd BLAST | 120 | |
PropeptideiPRO_0000017201 | 121 – 125 | Removed in mature form | 5 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | Phosphoserine; by PKABy similarity | 1 | |
Lipidationi | 120 | Phosphatidylethanolamine amidated glycineBy similarity | 1 |
Post-translational modificationi
The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.By similarity
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 120 – 121 | Cleavage; by ATG4B | 2 |
Keywords - PTMi
Lipoprotein, PhosphoproteinProteomic databases
MaxQBi | Q9CQV6. |
PaxDbi | Q9CQV6. |
PRIDEi | Q9CQV6. |
PTM databases
iPTMneti | Q9CQV6. |
PhosphoSitePlusi | Q9CQV6. |
SwissPalmi | Q9CQV6. |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031812. |
ExpressionAtlasi | Q9CQV6. baseline and differential. |
Genevisiblei | Q9CQV6. MM. |
Interactioni
Subunit structurei
3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Interacts with FYCO1 (via C-terminus). Interacts with TP53INP1 and TP53INP2 (By similarity). Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG4B, MAPK15 and BNIP3. Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2. Interacts with TBC1D5. Found in a complex with UBQLN1 and UBQLN2. Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4. Interacts with ATG13. Interacts with RETREG1, RETREG2 and RETREG3 (By similarity). Interacts with PLCL1; the interaction inhibits autophagosome formation (PubMed:23399561).By similarity2 Publications
GO - Molecular functioni
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGridi | 212190. 5 interactors. |
DIPi | DIP-61580N. |
ELMi | Q9CQV6. |
IntActi | Q9CQV6. 5 interactors. |
MINTi | Q9CQV6. |
STRINGi | 10090.ENSMUSP00000034270. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 7 – 10 | Combined sources | 4 | |
Helixi | 13 – 26 | Combined sources | 14 | |
Beta strandi | 30 – 37 | Combined sources | 8 | |
Beta strandi | 51 – 55 | Combined sources | 5 | |
Helixi | 60 – 70 | Combined sources | 11 | |
Beta strandi | 80 – 83 | Combined sources | 4 | |
Turni | 84 – 86 | Combined sources | 3 | |
Helixi | 95 – 102 | Combined sources | 8 | |
Beta strandi | 109 – 115 | Combined sources | 7 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5WRD | X-ray | 1.90 | A/B | 1-125 | [»] | |
ProteinModelPortali | Q9CQV6. | |||||
SMRi | Q9CQV6. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATG8 family.Curated
Phylogenomic databases
eggNOGi | KOG1654. Eukaryota. ENOG4111JAT. LUCA. |
GeneTreei | ENSGT00390000012937. |
HOGENOMi | HOG000232034. |
HOVERGENi | HBG051706. |
InParanoidi | Q9CQV6. |
KOi | K10435. |
PhylomeDBi | Q9CQV6. |
TreeFami | TF312964. |
Family and domain databases
CDDi | cd01611. GABARAP. 1 hit. |
InterProi | View protein in InterPro IPR004241. Atg8-like. IPR029071. Ubiquitin-like_domsf. |
PANTHERi | PTHR10969. PTHR10969. 1 hit. |
Pfami | View protein in Pfam PF02991. Atg8. 1 hit. |
SUPFAMi | SSF54236. SSF54236. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9CQV6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT
60 70 80 90 100
KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE
110 120
SERDEDGFLY MVYASQETFG TAMAV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | V → L in BAB22641 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF255953 mRNA. Translation: AAL83723.1. AK002795 mRNA. Translation: BAB22364.1. AK003106 mRNA. Translation: BAB22569.1. AK003205 mRNA. Translation: BAB22641.1. AK003558 mRNA. Translation: BAB22855.1. AK012604 mRNA. Translation: BAB28350.1. AK132329 mRNA. Translation: BAE21108.1. AK151614 mRNA. Translation: BAE30551.1. BC068180 mRNA. Translation: AAH68180.1. |
CCDSi | CCDS22726.1. |
RefSeqi | NP_080436.1. NM_026160.4. |
UniGenei | Mm.28357. |
Genome annotation databases
Ensembli | ENSMUST00000034270; ENSMUSP00000034270; ENSMUSG00000031812. |
GeneIDi | 67443. |
KEGGi | mmu:67443. |
UCSCi | uc009nrw.1. mouse. |
Similar proteinsi
Entry informationi
Entry namei | MLP3B_MOUSE | |
Accessioni | Q9CQV6Primary (citable) accession number: Q9CQV6 Secondary accession number(s): Q3U9W5, Q9D1R0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2002 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 28, 2018 | |
This is version 143 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |