Map1lc3b

Functionⁱ

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.By similarity

GO - Molecular functionⁱ

ubiquitin protein ligase binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

autophagosome assembly Source: GO_Central
autophagosome maturation Source: MGI
autophagy
Source: CAFA
Inferred from direct assayⁱ
- 23629966
autophagy of mitochondrion Source: MGI
cellular response to nitrogen starvation Source: GO_Central
cellular response to starvation Source: MGI
macroautophagy Source: MGI
positive regulation of mucus secretion
Source: MGI
Inferred from mutant phenotypeⁱ
- 24185898

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Autophagy, Ubl conjugation pathway

Biological process

Autophagy, Ubl conjugation pathway

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1632852. Macroautophagy. R-MMU-5205685. Pink/Parkin Mediated Mitophagy. R-MMU-8854214. TBC/RABGAPs. R-MMU-8934903. Receptor Mediated Mitophagy.

Reactomeⁱ

R-MMU-1632852. Macroautophagy.
R-MMU-5205685. Pink/Parkin Mediated Mitophagy.
R-MMU-8854214. TBC/RABGAPs.
R-MMU-8934903. Receptor Mediated Mitophagy.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Microtubule-associated proteins 1A/1B light chain 3B Alternative name(s): Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B MAP1 light chain 3-like protein 2 MAP1A/MAP1B light chain 3 B Short name: MAP1A/MAP1B LC3 B Microtubule-associated protein 1 light chain 3 beta
Gene namesⁱ	Name:Map1lc3b Synonyms:Map1alc3, Map1lc3
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 8

Organism-specific databases

MGIⁱ	MGI:1914693. Map1lc3b.

Keywords - Cellular componentⁱ

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000017200	1 – 120	Microtubule-associated proteins 1A/1B light chain 3BAdd BLAST		120
PropeptideⁱPRO_0000017201	121 – 125	Removed in mature form		5

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	12	Phosphoserine; by PKABy similarity		1
Lipidationⁱ	120	Phosphatidylethanolamine amidated glycineBy similarity		1

Post-translational modificationⁱ

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.By similarity

Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	120 – 121	Cleavage; by ATG4B		2

Keywords - PTMⁱ

Lipoprotein, Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9CQV6.
PaxDbⁱ	Q9CQV6.
PRIDEⁱ	Q9CQV6.

PTM databases

iPTMnetⁱ	Q9CQV6.
PhosphoSitePlusⁱ	Q9CQV6.
SwissPalmⁱ	Q9CQV6.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000031812.
ExpressionAtlasⁱ	Q9CQV6. baseline and differential.
Genevisibleⁱ	Q9CQV6. MM.

Interactionⁱ

Subunit structureⁱ

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Interacts with FYCO1 (via C-terminus). Interacts with TP53INP1 and TP53INP2 (By similarity). Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG4B, MAPK15 and BNIP3. Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2. Interacts with TBC1D5. Found in a complex with UBQLN1 and UBQLN2. Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4. Interacts with ATG13. Interacts with RETREG1, RETREG2 and RETREG3 (By similarity). Interacts with PLCL1; the interaction inhibits autophagosome formation (PubMed:23399561).By similarity2 Publications

GO - Molecular functionⁱ

ubiquitin protein ligase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	212190. 5 interactors.
Database of interacting proteins More...DIPⁱ	DIP-61580N.
ELMⁱ	Q9CQV6.
IntActⁱ	Q9CQV6. 5 interactors.
Molecular INTeraction database More...MINTⁱ	Q9CQV6.
STRINGⁱ	10090.ENSMUSP00000034270.

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	7 – 10	Combined sources	4
Helixⁱ	13 – 26	Combined sources	14
Beta strandⁱ	30 – 37	Combined sources	8
Beta strandⁱ	51 – 55	Combined sources	5
Helixⁱ	60 – 70	Combined sources	11
Beta strandⁱ	80 – 83	Combined sources	4
Turnⁱ	84 – 86	Combined sources	3
Helixⁱ	95 – 102	Combined sources	8
Beta strandⁱ	109 – 115	Combined sources	7

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5WRD	X-ray	1.90	A/B	1-125	[»]
ProteinModelPortalⁱ	Q9CQV6.
SMRⁱ	Q9CQV6.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1654. Eukaryota. ENOG4111JAT. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000012937.
HOGENOMⁱ	HOG000232034.
HOVERGENⁱ	HBG051706.
InParanoidⁱ	Q9CQV6.
KEGG Orthology (KO) More...KOⁱ	K10435.
PhylomeDBⁱ	Q9CQV6.
TreeFamⁱ	TF312964.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01611. GABARAP. 1 hit.
InterProⁱ	View protein in InterPro IPR004241. Atg8-like. IPR029071. Ubiquitin-like_domsf.
PANTHERⁱ	PTHR10969. PTHR10969. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02991. Atg8. 1 hit.
SUPFAMⁱ	SSF54236. SSF54236. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9CQV6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT 
        60         70         80         90        100
KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE 
       110        120 
SERDEDGFLY MVYASQETFG TAMAV

Length:125

Mass (Da):14,617

Last modified:January 23, 2007 - v3

Checksum:ⁱ520E29028163FA8D

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	89	V → L in BAB22641 (PubMed:16141072).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF255953 mRNA. Translation: AAL83723.1. AK002795 mRNA. Translation: BAB22364.1. AK003106 mRNA. Translation: BAB22569.1. AK003205 mRNA. Translation: BAB22641.1. AK003558 mRNA. Translation: BAB22855.1. AK012604 mRNA. Translation: BAB28350.1. AK132329 mRNA. Translation: BAE21108.1. AK151614 mRNA. Translation: BAE30551.1. BC068180 mRNA. Translation: AAH68180.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS22726.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_080436.1. NM_026160.4.
UniGeneⁱ	Mm.28357.

Genome annotation databases

Ensemblⁱ	ENSMUST00000034270; ENSMUSP00000034270; ENSMUSG00000031812.
GeneIDⁱ	67443.
KEGGⁱ	mmu:67443.
UCSC genome browser More...UCSCⁱ	uc009nrw.1. mouse.

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9CQV6	UPI0003508397 UPI000350835B UPI0009FB94AB UPI0003D6D8C8 UPI0006AD09A1 UPI0005840180 UPI000A2BFC07 UPI000B5ABD73 UPI0001758A5C H2NRQ3 +61	Homo sapiens (Human) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Callithrix jacchus (White-tufted-ear marmoset) Rattus norvegicus (Rat) Octodon degus (Degu) (Sciurus degus) Chinchilla lanigera (Long-tailed chinchilla) (Chinchilla villidera) Oryctolagus cuniculus (Rabbit) Heterocephalus glaber (Naked mole rat) Ailuropoda melanoleuca (Giant panda) And more	125	UniRef90_Q9CQV6	Cluster: Microtubule-associated proteins 1A/1B light chain 3B	72

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9CQV6	UPI0003508397 UPI000350835B UPI0009FB94AB UPI0006AD09A1 UPI0003D6D8C8 UPI0005840180 UPI000A2BFC07 UPI000B5ABD73 UPI0001758A5C UPI0004F03B54 +134	Homo sapiens (Human) Callithrix jacchus (White-tufted-ear marmoset) Rattus norvegicus (Rat) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Octodon degus (Degu) (Sciurus degus) Heterocephalus glaber (Naked mole rat) Oryctolagus cuniculus (Rabbit) Chinchilla lanigera (Long-tailed chinchilla) (Chinchilla villidera) Ailuropoda melanoleuca (Giant panda) And more	125	UniRef50_Q9CQV6	Cluster: Microtubule-associated proteins 1A/1B light chain 3B	145

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF255953 mRNA. Translation: AAL83723.1. AK002795 mRNA. Translation: BAB22364.1. AK003106 mRNA. Translation: BAB22569.1. AK003205 mRNA. Translation: BAB22641.1. AK003558 mRNA. Translation: BAB22855.1. AK012604 mRNA. Translation: BAB28350.1. AK132329 mRNA. Translation: BAE21108.1. AK151614 mRNA. Translation: BAE30551.1. BC068180 mRNA. Translation: AAH68180.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS22726.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_080436.1. NM_026160.4.
UniGeneⁱ	Mm.28357.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5WRD	X-ray	1.90	A/B	1-125	[»]
ProteinModelPortalⁱ	Q9CQV6.
SMRⁱ	Q9CQV6.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	212190. 5 interactors.
Database of interacting proteins More...DIPⁱ	DIP-61580N.
ELMⁱ	Q9CQV6.
IntActⁱ	Q9CQV6. 5 interactors.
Molecular INTeraction database More...MINTⁱ	Q9CQV6.
STRINGⁱ	10090.ENSMUSP00000034270.

PTM databases

iPTMnetⁱ	Q9CQV6.
PhosphoSitePlusⁱ	Q9CQV6.
SwissPalmⁱ	Q9CQV6.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9CQV6.
PaxDbⁱ	Q9CQV6.
PRIDEⁱ	Q9CQV6.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000034270; ENSMUSP00000034270; ENSMUSG00000031812.
GeneIDⁱ	67443.
KEGGⁱ	mmu:67443.
UCSC genome browser More...UCSCⁱ	uc009nrw.1. mouse.

Organism-specific databases

CTDⁱ	81631.
MGIⁱ	MGI:1914693. Map1lc3b.

Phylogenomic databases

eggNOGⁱ	KOG1654. Eukaryota. ENOG4111JAT. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000012937.
HOGENOMⁱ	HOG000232034.
HOVERGENⁱ	HBG051706.
InParanoidⁱ	Q9CQV6.
KEGG Orthology (KO) More...KOⁱ	K10435.
PhylomeDBⁱ	Q9CQV6.
TreeFamⁱ	TF312964.

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1632852. Macroautophagy. R-MMU-5205685. Pink/Parkin Mediated Mitophagy. R-MMU-8854214. TBC/RABGAPs. R-MMU-8934903. Receptor Mediated Mitophagy.

Reactomeⁱ

R-MMU-1632852. Macroautophagy.
R-MMU-5205685. Pink/Parkin Mediated Mitophagy.
R-MMU-8854214. TBC/RABGAPs.
R-MMU-8934903. Receptor Mediated Mitophagy.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9CQV6.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000031812.
ExpressionAtlasⁱ	Q9CQV6. baseline and differential.
Genevisibleⁱ	Q9CQV6. MM.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01611. GABARAP. 1 hit.
InterProⁱ	View protein in InterPro IPR004241. Atg8-like. IPR029071. Ubiquitin-like_domsf.
PANTHERⁱ	PTHR10969. PTHR10969. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02991. Atg8. 1 hit.
SUPFAMⁱ	SSF54236. SSF54236. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MLP3B_MOUSE
Accessionⁱ	Q9CQV6Primary (citable) accession number: Q9CQV6 Secondary accession number(s): Q3U9W5, Q9D1R0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
	Last sequence update:	January 23, 2007
	Last modified:	February 28, 2018
	This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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UniParc

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Supporting data

UniProtKB - Q9CQV6 (MLP3B_MOUSE)

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Microtubule-associated proteins 1A/1B light chain 3B

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Other locations

Cytoskeleton

Cytosol

Lysosome

Mitochondrion

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ