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Protein

Developmental pluripotency-associated protein 5A

Gene

Dppa5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maintenance of embryonic stem (ES) cell pluripotency. Dispensable for self-renewal of pluripotent ES cells and establishment of germ cells. Associates with specific target mRNAs.1 Publication

GO - Molecular functioni

  • mRNA binding Source: MGI

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • regulation of gene expression Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental pluripotency-associated protein 5A
Short name:
mDppa5
Alternative name(s):
ES cell-associated transcript 2 protein
Embryonal stem cell-specific gene 1 protein
Short name:
ESG-1
Protein pH 34
Gene namesi
Name:Dppa5a
Synonyms:Dppa5, Ecat2, Esg1, Ph34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:101800. Dppa5a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice develop normally and are fertile. ES cells derived from these mice demonstrate normal morphology, proliferation and differentiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 118118Developmental pluripotency-associated protein 5APRO_0000311976Add
BLAST

Proteomic databases

PaxDbiQ9CQS7.
PRIDEiQ9CQS7.

2D gel databases

REPRODUCTION-2DPAGEQ9CQS7.

PTM databases

iPTMnetiQ9CQS7.
PhosphoSiteiQ9CQS7.

Expressioni

Developmental stagei

Pluripotent cell-specific. Expressed in zygotes, cleavage-stage embryos and blastocysts, embryonic stem (ES) and embryonic germ (EG) cells. Detected in both the trophectoderm (TE) and inner cell mass (ICM) of blastocysts. More abundant in the ICM than TE at 3.5 dpc blastocyst stage. Expressed in primordial germ (PGC) cells from 10.5 to 13.5 dpc. Expressed in developing gonads from 11.5 to 15.5 dpc. Progressively undetectable in ovary from 13.5 to 15.5 dpc. Undetectable in testis after 15.5 dpc. Not expressed in somatic cells at 13.5 dpc (at protein level). Expressed in embryonic stem (ES) and embryonic carcinoma (EC) cells. Not detected during the differentiation of stem cells or midgestation embryos nor in neonatal tissues. Weakly expressed or not detected in oocytes and fertilized eggs.7 Publications

Inductioni

Down-regulated by retinoic acid in embryonic carcinoma (EC) cells and in developing germ cells.2 Publications

Gene expression databases

BgeeiQ9CQS7.
CleanExiMM_DPPA5A.
GenevisibleiQ9CQS7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071881.

Structurei

3D structure databases

ProteinModelPortaliQ9CQS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 8663KH; atypicalAdd
BLAST

Sequence similaritiesi

Belongs to the KHDC1 family.Curated
Contains 1 KH domain.Curated

Phylogenomic databases

eggNOGiENOG410IZIS. Eukaryota.
ENOG4111AYN. LUCA.
GeneTreeiENSGT00390000011646.
HOGENOMiHOG000081506.
HOVERGENiHBG107820.
InParanoidiQ9CQS7.
OMAiFAHISTW.
OrthoDBiEOG7B5WZ3.
PhylomeDBiQ9CQS7.
TreeFamiTF338690.

Family and domain databases

InterProiIPR004088. KH_dom_type_1.
IPR031952. MOEP19_KH-like.
[Graphical view]
PfamiPF16005. MOEP19. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CQS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVTLVTRKD IPPWVKVPED LKDPEVFQVQ SLVLKYLFGP QGSRMSHIEQ
60 70 80 90 100
VSQAMFELKN LESPEELIEV FIYGSQNNKI RAKWMLQSMA ERYHLRQQKG
110
VLKLEESMKT LELGQCIE
Length:118
Mass (Da):13,810
Last modified:June 1, 2003 - v2
Checksum:i30233CB5C536AC55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF490349 mRNA. Translation: AAO84507.1.
AK010519 mRNA. Translation: BAB27001.2.
AK010584 mRNA. Translation: BAB27045.2.
AK145761 mRNA. Translation: BAE26633.1.
BC092353 mRNA. Translation: AAH92353.1.
BC092354 mRNA. Translation: AAH92354.1.
BC095979 mRNA. Translation: AAH95979.1.
CCDSiCCDS23360.1.
PIRiA55058.
RefSeqiNP_079550.2. NM_025274.3.
UniGeneiMm.139314.
Mm.440500.

Genome annotation databases

EnsembliENSMUST00000071991; ENSMUSP00000071881; ENSMUSG00000060461.
GeneIDi434423.
KEGGimmu:434423.
UCSCiuc009qug.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF490349 mRNA. Translation: AAO84507.1.
AK010519 mRNA. Translation: BAB27001.2.
AK010584 mRNA. Translation: BAB27045.2.
AK145761 mRNA. Translation: BAE26633.1.
BC092353 mRNA. Translation: AAH92353.1.
BC092354 mRNA. Translation: AAH92354.1.
BC095979 mRNA. Translation: AAH95979.1.
CCDSiCCDS23360.1.
PIRiA55058.
RefSeqiNP_079550.2. NM_025274.3.
UniGeneiMm.139314.
Mm.440500.

3D structure databases

ProteinModelPortaliQ9CQS7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071881.

PTM databases

iPTMnetiQ9CQS7.
PhosphoSiteiQ9CQS7.

2D gel databases

REPRODUCTION-2DPAGEQ9CQS7.

Proteomic databases

PaxDbiQ9CQS7.
PRIDEiQ9CQS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071991; ENSMUSP00000071881; ENSMUSG00000060461.
GeneIDi434423.
KEGGimmu:434423.
UCSCiuc009qug.2. mouse.

Organism-specific databases

CTDi434423.
MGIiMGI:101800. Dppa5a.

Phylogenomic databases

eggNOGiENOG410IZIS. Eukaryota.
ENOG4111AYN. LUCA.
GeneTreeiENSGT00390000011646.
HOGENOMiHOG000081506.
HOVERGENiHBG107820.
InParanoidiQ9CQS7.
OMAiFAHISTW.
OrthoDBiEOG7B5WZ3.
PhylomeDBiQ9CQS7.
TreeFamiTF338690.

Miscellaneous databases

NextBioi409626.
PROiQ9CQS7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CQS7.
CleanExiMM_DPPA5A.
GenevisibleiQ9CQS7. MM.

Family and domain databases

InterProiIPR004088. KH_dom_type_1.
IPR031952. MOEP19_KH-like.
[Graphical view]
PfamiPF16005. MOEP19. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Changes in gene expression following exposure of nulli-SCCl murine embryonal carcinoma cells to inducers of differentiation: characterization of a down-regulated mRNA."
    Astigiano S., Barkai U., Abarzua P., Tan S.C., Harper M.I., Sherman M.I.
    Differentiation 46:61-67(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
  2. "Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei."
    Bortvin A., Eggan K., Skaletsky H., Akutsu H., Berry D.L., Yanagimachi R., Page D.C., Jaenisch R.
    Development 130:1673-1680(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Blastocyst and Embryonic stem cell.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic germ cell.
  5. "Cloning of embryonal stem cell-specific genes: characterization of the transcriptionally controlled gene esg-1."
    Bierbaum P., MacLean-Hunter S., Ehlert F., Moeroey T., Mueller R.
    Cell Growth Differ. 5:37-46(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity."
    Tanaka T.S., Kunath T., Kimber W.L., Jaradat S.A., Stagg C.A., Usuda M., Yokota T., Niwa H., Rossant J., Ko M.S.
    Genome Res. 12:1921-1928(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  7. "Identification of developmental pluripotency associated 5 expression in human pluripotent stem cells."
    Kim S.-K., Suh M.R., Yoon H.S., Lee J.B., Oh S.K., Moon S.-Y., Moon S.H., Lee J.Y., Hwang J.H., Cho W.J., Kim K.-S.
    Stem Cells 23:458-462(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, DEVELOPMENTAL STAGE.
  8. "Analysis of Esg1 expression in pluripotent cells and the germline reveals similarities with Oct4 and Sox2 and differences between human pluripotent cell lines."
    Western P., Maldonado-Saldivia J., van den Bergen J., Hajkova P., Saitou M., Barton S., Surani M.A.
    Stem Cells 23:1436-1442(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  9. "Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5)."
    Amano H., Itakura K., Maruyama M., Ichisaka T., Nakagawa M., Yamanaka S.
    BMC Dev. Biol. 6:11-11(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  10. "Esg1, expressed exclusively in preimplantation embryos, germline, and embryonic stem cells, is a putative RNA-binding protein with broad RNA targets."
    Tanaka T.S., Lopez de Silanes I., Sharova L.V., Akutsu H., Yoshikawa T., Amano H., Yamanaka S., Gorospe M., Ko M.S.
    Dev. Growth Differ. 48:381-390(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, RNA-BINDING, DEVELOPMENTAL STAGE.
  11. "Atypical structure and phylogenomic evolution of the new eutherian oocyte-and embryo-expressed KHDC1/DPPA5/ECAT1/OOEP gene family."
    Pierre A., Gautier M., Callebaut I., Bontoux M., Jeanpierre E., Pontarotti P., Monget P.
    Genomics 90:583-594(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiDPA5A_MOUSE
AccessioniPrimary (citable) accession number: Q9CQS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.