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Protein

Gamma-secretase subunit PEN-2

Gene

Psenen

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein) (PubMed:12522139, PubMed:24941111). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity (PubMed:12522139, PubMed:24941111).Curated2 Publications

GO - Biological processi

Keywordsi

Biological processNotch signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-1251985. Nuclear signaling by ERBB4.
R-MMU-193692. Regulated proteolysis of p75NTR.
R-MMU-1980148. Signaling by NOTCH3.
R-MMU-1980150. Signaling by NOTCH4.
R-MMU-205043. NRIF signals cell death from the nucleus.
R-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
R-MMU-3928665. EPH-ephrin mediated repulsion of cells.

Protein family/group databases

TCDBi9.B.47.1.1. the -secretase (-secretase) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-secretase subunit PEN-2
Alternative name(s):
Presenilin enhancer protein 2
Gene namesi
Name:Psenen
Synonyms:Pen2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913590. Psenen.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17CytoplasmicBy similarityAdd BLAST17
Intramembranei18 – 36HelicalBy similarityAdd BLAST19
Topological domaini37 – 57CytoplasmicBy similarityAdd BLAST21
Transmembranei58 – 78HelicalBy similarityAdd BLAST21
Topological domaini79 – 101LumenalBy similarityAdd BLAST23

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001909011 – 101Gamma-secretase subunit PEN-2Add BLAST101

Proteomic databases

MaxQBiQ9CQR7.
PaxDbiQ9CQR7.
PeptideAtlasiQ9CQR7.
PRIDEiQ9CQR7.
TopDownProteomicsiQ9CQR7.

PTM databases

PhosphoSitePlusiQ9CQR7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036835.
CleanExiMM_PSENEN.
ExpressionAtlasiQ9CQR7. baseline and differential.
GenevisibleiQ9CQR7. MM.

Interactioni

Subunit structurei

The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2.2 Publications

Protein-protein interaction databases

BioGridi211397. 2 interactors.
DIPiDIP-36333N.
IntActiQ9CQR7. 3 interactors.
STRINGi10090.ENSMUSP00000044682.

Structurei

3D structure databases

ProteinModelPortaliQ9CQR7.
SMRiQ9CQR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PEN-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3402. Eukaryota.
ENOG4111W09. LUCA.
GeneTreeiENSGT00390000016319.
HOGENOMiHOG000006914.
HOVERGENiHBG045628.
InParanoidiQ9CQR7.
KOiK06170.
OMAiWFFQEAF.
OrthoDBiEOG091G0X8A.
PhylomeDBiQ9CQR7.
TreeFamiTF313116.

Family and domain databases

InterProiView protein in InterPro
IPR019379. Gamma_Secretase_Asp_P_PEN2.
PANTHERiPTHR16318. PTHR16318. 1 hit.
PfamiView protein in Pfam
PF10251. PEN-2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CQR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLERVSNEE KLNLCRKYYL GGFAFLPFLW LVNIFWFFRE AFLAPAYTEQ
60 70 80 90 100
SQIKGYVWRS AVGFLFWVII LATWITIFQI YRPRWGALGD YLSFTIPLGT

P
Length:101
Mass (Da):11,999
Last modified:June 1, 2001 - v1
Checksum:i6596CA2984FBCC59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002440 mRNA. Translation: BAB22102.1.
AK006284 mRNA. Translation: BAB24503.1.
AK007621 mRNA. Translation: BAB25141.1.
BC024347 mRNA. Translation: AAH24347.1.
BC081443 mRNA. Translation: AAH81443.1.
CCDSiCCDS21099.1.
RefSeqiNP_079774.1. NM_025498.2.
UniGeneiMm.86557.

Genome annotation databases

EnsembliENSMUST00000043898; ENSMUSP00000044682; ENSMUSG00000036835.
ENSMUST00000207747; ENSMUSP00000146675; ENSMUSG00000036835.
GeneIDi66340.
KEGGimmu:66340.
UCSCiuc009gez.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPEN2_MOUSE
AccessioniPrimary (citable) accession number: Q9CQR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: June 1, 2001
Last modified: July 5, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

3D-structure analysis of the human homolog indicates that the membrane topology differs from the predictions. Contrary to predictions, the N-terminus contains two short helices that dip into the membrane, but do not cross it. The C-terminus contains the single transmembrane helix. This gives rise to a topology where the N-terminus is cytoplasmic and the C-terminus is lumenal.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families