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Protein

Gem-associated protein 2

Gene

Gemin2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-191859. snRNP Assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Gem-associated protein 2
Short name:
Gemin-2
Alternative name(s):
Component of gems 2
Survival of motor neuron protein-interacting protein 1
Short name:
SMN-interacting protein 1
Gene namesi
Name:Gemin2
Synonyms:Sip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1913853. Gemin2.

Subcellular locationi

  • Nucleusgem By similarity
  • Cytoplasm By similarity

  • Note: Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • Gemini of coiled bodies Source: UniProtKB-SubCell
  • intracellular Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
  • SMN complex Source: UniProtKB
  • SMN-Sm protein complex Source: UniProtKB
  • spliceosomal complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Gem-associated protein 2PRO_0000087456Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701PhosphoserineBy similarity
Modified residuei155 – 1551PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9CQQ4.
MaxQBiQ9CQQ4.
PaxDbiQ9CQQ4.
PRIDEiQ9CQQ4.

PTM databases

PhosphoSiteiQ9CQQ4.

Expressioni

Gene expression databases

BgeeiQ9CQQ4.
GenevisibleiQ9CQQ4. MM.

Interactioni

Subunit structurei

Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with GEMIN5 (By similarity).By similarity

Protein-protein interaction databases

BioGridi211588. 6 interactions.
IntActiQ9CQQ4. 1 interaction.
STRINGi10090.ENSMUSP00000021379.

Structurei

3D structure databases

ProteinModelPortaliQ9CQQ4.
SMRiQ9CQQ4. Positions 11-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi90 – 956Poly-Gln

Sequence similaritiesi

Belongs to the gemin-2 family.Curated

Phylogenomic databases

eggNOGiENOG410IJ8K. Eukaryota.
ENOG410YM8D. LUCA.
GeneTreeiENSGT00390000013814.
HOGENOMiHOG000008019.
HOVERGENiHBG051719.
InParanoidiQ9CQQ4.
KOiK13130.
OMAiNHWKSQQ.
OrthoDBiEOG7XH6RF.
PhylomeDBiQ9CQQ4.
TreeFamiTF105864.

Family and domain databases

InterProiIPR017364. GEMIN2.
[Graphical view]
PIRSFiPIRSF038038. SMN_Gemin2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CQQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWVPAESAV EELMPRLLPV EPCDLTEGFD PSVPPRTPQE YLRRVQIEAA
60 70 80 90 100
QCPDVVVAQI DPKKLKRKQS VNISLSGCQP APEGYSPTLQ WQQQQVAHFS
110 120 130 140 150
TVRQSVHKHR NHWKSQQLDS NVAMPKSEDE EGWKKFCLGE RLCAEGATGP
160 170 180 190 200
STEESPGIDY VQVGFPPLLS IVSRMNQTTI TSVLEYLSNW FGERDFTPEL
210 220 230 240 250
GRWFYALLAC LEKPLLPEAH SLIRQLARRC SEVRLLVGSK DDERVPALNL
260
LICLVSRYFD QRDLADEPS
Length:269
Mass (Da):30,441
Last modified:June 1, 2001 - v1
Checksum:i6BB65AC800F8B329
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti241 – 2411D → E in BAB24321 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005928 mRNA. Translation: BAB24321.1.
AK007515 mRNA. Translation: BAB25083.1.
AK013414 mRNA. Translation: BAB28842.1.
CCDSiCCDS36457.1.
RefSeqiNP_079932.2. NM_025656.5.
UniGeneiMm.35353.

Genome annotation databases

EnsembliENSMUST00000021379; ENSMUSP00000021379; ENSMUSG00000060121.
GeneIDi66603.
KEGGimmu:66603.
UCSCiuc007npx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005928 mRNA. Translation: BAB24321.1.
AK007515 mRNA. Translation: BAB25083.1.
AK013414 mRNA. Translation: BAB28842.1.
CCDSiCCDS36457.1.
RefSeqiNP_079932.2. NM_025656.5.
UniGeneiMm.35353.

3D structure databases

ProteinModelPortaliQ9CQQ4.
SMRiQ9CQQ4. Positions 11-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211588. 6 interactions.
IntActiQ9CQQ4. 1 interaction.
STRINGi10090.ENSMUSP00000021379.

PTM databases

PhosphoSiteiQ9CQQ4.

Proteomic databases

EPDiQ9CQQ4.
MaxQBiQ9CQQ4.
PaxDbiQ9CQQ4.
PRIDEiQ9CQQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021379; ENSMUSP00000021379; ENSMUSG00000060121.
GeneIDi66603.
KEGGimmu:66603.
UCSCiuc007npx.2. mouse.

Organism-specific databases

CTDi8487.
MGIiMGI:1913853. Gemin2.

Phylogenomic databases

eggNOGiENOG410IJ8K. Eukaryota.
ENOG410YM8D. LUCA.
GeneTreeiENSGT00390000013814.
HOGENOMiHOG000008019.
HOVERGENiHBG051719.
InParanoidiQ9CQQ4.
KOiK13130.
OMAiNHWKSQQ.
OrthoDBiEOG7XH6RF.
PhylomeDBiQ9CQQ4.
TreeFamiTF105864.

Enzyme and pathway databases

ReactomeiR-MMU-191859. snRNP Assembly.

Miscellaneous databases

NextBioi322140.
PROiQ9CQQ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CQQ4.
GenevisibleiQ9CQQ4. MM.

Family and domain databases

InterProiIPR017364. GEMIN2.
[Graphical view]
PIRSFiPIRSF038038. SMN_Gemin2. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Pancreas and Testis.

Entry informationi

Entry nameiGEMI2_MOUSE
AccessioniPrimary (citable) accession number: Q9CQQ4
Secondary accession number(s): Q9DAD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2001
Last modified: March 16, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.