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Protein

E3 ubiquitin-protein ligase RING2

Gene

Rnf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed:15525528, PubMed:22325148, PubMed:28596365). H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals (PubMed:15525528, PubMed:28596365). May be involved in the initiation of both imprinted and random X inactivation (PubMed:15525528). Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:22325148, PubMed:16710298). PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed:15525528, PubMed:22325148, PubMed:16710298). E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed:16710298). Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (PubMed:15525528, PubMed:16710298). Plays a role in the transcriptional repression of genes that are required for pluripotency in embryonic stem cells, thereby contributing to differentiation of the ectodermal and endodermal germ layers (PubMed:22226355). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (PubMed:24034696).7 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.2 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri51 – 91RING-typeAdd BLAST41

GO - Molecular functioni

  • chromatin binding Source: MGI
  • RING-like zinc finger domain binding Source: MGI
  • ubiquitin protein ligase activity Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • anterior/posterior axis specification Source: MGI
  • gastrulation with mouth forming second Source: MGI
  • germ cell development Source: CACAO
  • histone H2A-K119 monoubiquitination Source: UniProtKB
  • histone H2A monoubiquitination Source: MGI
  • histone ubiquitination Source: MGI
  • mitotic cell cycle Source: MGI
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor, Transferase
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi6.3.2.19. 3474.
ReactomeiR-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-4551638. SUMOylation of chromatin organization proteins.
R-MMU-8939243. RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known.
R-MMU-8943724. Regulation of PTEN gene transcription.
R-MMU-8953750. Transcriptional Regulation by E2F6.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RING2 (EC:2.3.2.272 Publications)
Alternative name(s):
RING finger protein 1B
Short name:
RING1b
RING finger protein 2
RING-type E3 ubiquitin transferase RING2Curated
Gene namesi
Name:Rnf2
Synonyms:DinG, Ring1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1101759. Rnf2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000560392 – 336E3 ubiquitin-protein ligase RING2Add BLAST335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Cross-linki112Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residuei143PhosphoserineBy similarity1
Modified residuei168PhosphoserineBy similarity1
Cross-linki249Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki323Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Polyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9CQJ4.
MaxQBiQ9CQJ4.
PaxDbiQ9CQJ4.
PeptideAtlasiQ9CQJ4.
PRIDEiQ9CQJ4.

PTM databases

iPTMnetiQ9CQJ4.
PhosphoSitePlusiQ9CQJ4.

Expressioni

Tissue specificityi

Expressed in embryonic stem cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000026484.
CleanExiMM_RNF2.
ExpressionAtlasiQ9CQJ4. baseline and differential.
GenevisibleiQ9CQJ4. MM.

Interactioni

Subunit structurei

Component of chromatin-associated Polycomb (PcG) complexes (PubMed:22325148). Component of a number of PRC1-like complexes; these complexes contain either the polycomb group ring finger protein PCGF1, or PCGF2, or PCGF3, or BMI1, or PCGF5, or PCGF6 (PubMed:28596365, PubMed:16710298). Distinct PRC1-like complexes are composed of a RING1 subunit (RING1B or RING1A), one of the six PCGF proteins (PCGF1, PCGF2, PCGF3, BMI1, PCGF5 or PCGF6), one PHC protein (PHC1, PHC2 or PHC3) and one of the CBX proteins (CBX2, CBX4, CBX6, CBX7 or CBX8) (Probable). Part of a complex that contains RNF2, UB2D3 and BMI1; within that complex RNF2 and BMI1 form a tight heterodimer, where UB2D3 interacts only with RNF2. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (By similarity). Part of a complex that contains PCGF5, RNF2 and UBE2D3. Part of a complex that contains AUTS2, PCGF5, RNF2, CSNK2B AND RYBP (By similarity). Interacts with CBX6 and CBX8 (By similarity). Interacts with PHC1, PCGF2, RYBP, CBX7, CBX4, CBX2, RNF1/RING1, BMI1 and PHC2 (PubMed:12183370, PubMed:16024804, PubMed:16710298, PubMed:19170609, PubMed:9312051, PubMed:10369680, PubMed:22325148, PubMed:22226355). Interaction with RYBP and CBX7 is mutually exclusive; both compete for the same binding site on RNF2 (PubMed:22325148). Component of repressive BCOR complex containing a Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1 (By similarity). Interacts with CBX2 and PHC1 (PubMed:22226355). Interacts with CHTOP (PubMed:22872859). Interacts with AURKB (PubMed:24034696). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RNF1/RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2 (By similarity). Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with RYBP, HIP2 and TFCP2 (By similarity).By similarityCurated11 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202919. 124 interactors.
DIPiDIP-36109N.
IntActiQ9CQJ4. 59 interactors.
MINTiMINT-1341250.
STRINGi10090.ENSMUSP00000075476.

Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 25Combined sources5
Helixi46 – 49Combined sources4
Turni52 – 54Combined sources3
Beta strandi55 – 57Combined sources3
Beta strandi59 – 64Combined sources6
Turni65 – 67Combined sources3
Beta strandi70 – 72Combined sources3
Helixi73 – 81Combined sources9
Turni88 – 90Combined sources3
Helixi97 – 99Combined sources3
Beta strandi100 – 102Combined sources3
Helixi104 – 113Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKLX-ray2.00B1-159[»]
ProteinModelPortaliQ9CQJ4.
SMRiQ9CQJ4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9CQJ4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 179Interaction with HIP2By similarityAdd BLAST178
Regioni93 – 98Interaction with nucleosomes via an acidic patch on histone H2A and histone H2BBy similarity6

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri51 – 91RING-typeAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0311. Eukaryota.
ENOG410XQ5G. LUCA.
GeneTreeiENSGT00390000016977.
HOGENOMiHOG000273917.
HOVERGENiHBG079942.
InParanoidiQ9CQJ4.
KOiK10695.
OMAiFNQTQSQ.
OrthoDBiEOG091G06VO.
PhylomeDBiQ9CQJ4.
TreeFamiTF105501.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF16207. RAWUL. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQAVQTNGT QPLSKTWELS LYELQRTPQE AITDGLEIVV SPRSLHSELM
60 70 80 90 100
CPICLDMLKN TMTTKECLHR FCADCIITAL RSGNKECPTC RKKLVSKRSL
110 120 130 140 150
RPDPNFDALI SKIYPSRDEY EAHQERVLAR INKHNNQQAL SHSIEEGLKI
160 170 180 190 200
QAMNRLQRGK KQQIENGSGA EDNGDSSHCS NASTHSNQEA GPSNKRTKTS
210 220 230 240 250
DDSGLELDNN NAAVAIDPVM DGASEIELVF RPHPTLMEKD DSAQTRYIKT
260 270 280 290 300
SGNATVDHLS KYLAVRLALE ELRSKGESNQ MNLDTASEKQ YTIYIATASG
310 320 330
QFTVLNGSFS LELVSEKYWK VNKPMELYYA PTKEHK
Length:336
Mass (Da):37,623
Last modified:June 1, 2001 - v1
Checksum:i84BA5B3E044DB7EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253N → H in BAC41075 (PubMed:16141072).Curated1
Sequence conflicti299S → T in BAC41075 (PubMed:16141072).Curated1
Sequence conflicti304V → L in BAC41075 (PubMed:16141072).Curated1
Sequence conflicti332 – 336TKEHK → FTASGNVR in CAA73380 (PubMed:9312051).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12880 mRNA. Translation: CAA73380.1.
Y12783 mRNA. Translation: CAA73321.1.
AK145767 mRNA. Translation: BAE26638.1.
AK012358 mRNA. Translation: BAB28186.1.
AK013124 mRNA. Translation: BAB28663.1.
AK030319 mRNA. Translation: BAC26898.1.
AK090066 mRNA. Translation: BAC41075.1.
CT010297 mRNA. Translation: CAJ18505.1.
BC020122 mRNA. Translation: AAH20122.1.
CCDSiCCDS15362.1.
RefSeqiNP_035407.1. NM_011277.2.
UniGeneiMm.474038.

Genome annotation databases

EnsembliENSMUST00000076110; ENSMUSP00000075476; ENSMUSG00000026484.
ENSMUST00000187048; ENSMUSP00000140896; ENSMUSG00000026484.
GeneIDi19821.
KEGGimmu:19821.
UCSCiuc007cyx.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiRING2_MOUSE
AccessioniPrimary (citable) accession number: Q9CQJ4
Secondary accession number(s): O35699
, O35729, Q4FJV5, Q8C1X8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: November 22, 2017
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references