Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9CQI9

- MED30_MOUSE

UniProt

Q9CQI9 - MED30_MOUSE

Protein

Mediator of RNA polymerase II transcription subunit 30

Gene

Med30

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. receptor activity Source: Ensembl
    3. RNA polymerase II transcription cofactor activity Source: Ensembl

    GO - Biological processi

    1. androgen receptor signaling pathway Source: Ensembl
    2. positive regulation of transcription, DNA-templated Source: Ensembl
    3. stem cell maintenance Source: MGI
    4. transcription initiation from RNA polymerase II promoter Source: Ensembl

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.
    REACT_206529. Generic Transcription Pathway.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 30
    Alternative name(s):
    Mediator complex subunit 30
    Thyroid hormone receptor-associated protein 6
    Thyroid hormone receptor-associated protein complex 25 kDa component
    Short name:
    Trap25
    Gene namesi
    Name:Med30
    Synonyms:Thrap6, Trap25
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1917040. Med30.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: MGI
    2. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 178177Mediator of RNA polymerase II transcription subunit 30PRO_0000239407Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ9CQI9.
    PRIDEiQ9CQI9.

    PTM databases

    PhosphoSiteiQ9CQI9.

    Expressioni

    Gene expression databases

    BgeeiQ9CQI9.
    CleanExiMM_MED30.
    GenevestigatoriQ9CQI9.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MED22Q155284EBI-309220,EBI-394687From a different organism.

    Protein-protein interaction databases

    BioGridi213682. 2 interactions.
    IntActiQ9CQI9. 7 interactions.
    MINTiMINT-217060.
    STRINGi10090.ENSMUSP00000042204.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili134 – 17340Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 30 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG87821.
    GeneTreeiENSGT00390000010887.
    HOGENOMiHOG000006885.
    HOVERGENiHBG107279.
    InParanoidiQ9CQI9.
    KOiK15143.
    OMAiGSKHDDR.
    OrthoDBiEOG7C5MB0.
    PhylomeDBiQ9CQI9.
    TreeFamiTF324588.

    Family and domain databases

    InterProiIPR021019. Mediator_Med30_met.
    [Graphical view]
    PfamiPF11315. Med30. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9CQI9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPPLAPTG MASGPFGGPQ AQQAAREVNT ATLCRIGQET VQDIVYRTME    50
    IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD HLRQLSILFR KLRLVYDKCN 100
    ENCGGMDPIP VEQLIPYVDE DGSKNDDRAG PPRFASEERR EIVEVNKKLK 150
    QKNQQLKQIM DQLRNLIWDI NAMLAMRN 178
    Length:178
    Mass (Da):20,358
    Last modified:June 1, 2001 - v1
    Checksum:i8766D3C929C1E98D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK007739 mRNA. Translation: BAB25226.1.
    AK011104 mRNA. Translation: BAB27401.1.
    AK146250 mRNA. Translation: BAE27012.1.
    BC020113 mRNA. Translation: AAH20113.1.
    CCDSiCCDS27465.1.
    RefSeqiNP_081488.1. NM_027212.2.
    UniGeneiMm.195562.

    Genome annotation databases

    EnsembliENSMUST00000037115; ENSMUSP00000042204; ENSMUSG00000038622.
    GeneIDi69790.
    KEGGimmu:69790.
    UCSCiuc007vrg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK007739 mRNA. Translation: BAB25226.1 .
    AK011104 mRNA. Translation: BAB27401.1 .
    AK146250 mRNA. Translation: BAE27012.1 .
    BC020113 mRNA. Translation: AAH20113.1 .
    CCDSi CCDS27465.1.
    RefSeqi NP_081488.1. NM_027212.2.
    UniGenei Mm.195562.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 213682. 2 interactions.
    IntActi Q9CQI9. 7 interactions.
    MINTi MINT-217060.
    STRINGi 10090.ENSMUSP00000042204.

    PTM databases

    PhosphoSitei Q9CQI9.

    Proteomic databases

    PaxDbi Q9CQI9.
    PRIDEi Q9CQI9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037115 ; ENSMUSP00000042204 ; ENSMUSG00000038622 .
    GeneIDi 69790.
    KEGGi mmu:69790.
    UCSCi uc007vrg.1. mouse.

    Organism-specific databases

    CTDi 90390.
    MGIi MGI:1917040. Med30.

    Phylogenomic databases

    eggNOGi NOG87821.
    GeneTreei ENSGT00390000010887.
    HOGENOMi HOG000006885.
    HOVERGENi HBG107279.
    InParanoidi Q9CQI9.
    KOi K15143.
    OMAi GSKHDDR.
    OrthoDBi EOG7C5MB0.
    PhylomeDBi Q9CQI9.
    TreeFami TF324588.

    Enzyme and pathway databases

    Reactomei REACT_198602. PPARA activates gene expression.
    REACT_206529. Generic Transcription Pathway.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    NextBioi 330352.
    PROi Q9CQI9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9CQI9.
    CleanExi MM_MED30.
    Genevestigatori Q9CQI9.

    Family and domain databases

    InterProi IPR021019. Mediator_Med30_met.
    [Graphical view ]
    Pfami PF11315. Med30. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and DBA/2.
      Tissue: Liver and Pancreas.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiMED30_MOUSE
    AccessioniPrimary (citable) accession number: Q9CQI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3