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Q9CQI9

- MED30_MOUSE

UniProt

Q9CQI9 - MED30_MOUSE

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Protein
Mediator of RNA polymerase II transcription subunit 30
Gene
Med30, Thrap6, Trap25
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.

GO - Molecular functioni

  1. RNA polymerase II transcription cofactor activity Source: Ensembl
  2. protein binding Source: IntAct
  3. receptor activity Source: Ensembl

GO - Biological processi

  1. androgen receptor signaling pathway Source: Ensembl
  2. positive regulation of transcription, DNA-templated Source: Ensembl
  3. stem cell maintenance Source: MGI
  4. transcription initiation from RNA polymerase II promoter Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_198602. PPARA activates gene expression.
REACT_206529. Generic Transcription Pathway.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 30
Alternative name(s):
Mediator complex subunit 30
Thyroid hormone receptor-associated protein 6
Thyroid hormone receptor-associated protein complex 25 kDa component
Short name:
Trap25
Gene namesi
Name:Med30
Synonyms:Thrap6, Trap25
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1917040. Med30.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. mediator complex Source: MGI
  2. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 178177Mediator of RNA polymerase II transcription subunit 30
PRO_0000239407Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9CQI9.
PRIDEiQ9CQI9.

PTM databases

PhosphoSiteiQ9CQI9.

Expressioni

Gene expression databases

BgeeiQ9CQI9.
CleanExiMM_MED30.
GenevestigatoriQ9CQI9.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
MED22Q155284EBI-309220,EBI-394687From a different organism.

Protein-protein interaction databases

BioGridi213682. 2 interactions.
IntActiQ9CQI9. 7 interactions.
MINTiMINT-217060.
STRINGi10090.ENSMUSP00000042204.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili134 – 17340 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG87821.
GeneTreeiENSGT00390000010887.
HOGENOMiHOG000006885.
HOVERGENiHBG107279.
InParanoidiQ9CQI9.
KOiK15143.
OMAiGSKHDDR.
OrthoDBiEOG7C5MB0.
PhylomeDBiQ9CQI9.
TreeFamiTF324588.

Family and domain databases

InterProiIPR021019. Mediator_Med30_met.
[Graphical view]
PfamiPF11315. Med30. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQI9-1 [UniParc]FASTAAdd to Basket

« Hide

MSTPPLAPTG MASGPFGGPQ AQQAAREVNT ATLCRIGQET VQDIVYRTME    50
IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD HLRQLSILFR KLRLVYDKCN 100
ENCGGMDPIP VEQLIPYVDE DGSKNDDRAG PPRFASEERR EIVEVNKKLK 150
QKNQQLKQIM DQLRNLIWDI NAMLAMRN 178
Length:178
Mass (Da):20,358
Last modified:June 1, 2001 - v1
Checksum:i8766D3C929C1E98D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK007739 mRNA. Translation: BAB25226.1.
AK011104 mRNA. Translation: BAB27401.1.
AK146250 mRNA. Translation: BAE27012.1.
BC020113 mRNA. Translation: AAH20113.1.
CCDSiCCDS27465.1.
RefSeqiNP_081488.1. NM_027212.2.
UniGeneiMm.195562.

Genome annotation databases

EnsembliENSMUST00000037115; ENSMUSP00000042204; ENSMUSG00000038622.
GeneIDi69790.
KEGGimmu:69790.
UCSCiuc007vrg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK007739 mRNA. Translation: BAB25226.1 .
AK011104 mRNA. Translation: BAB27401.1 .
AK146250 mRNA. Translation: BAE27012.1 .
BC020113 mRNA. Translation: AAH20113.1 .
CCDSi CCDS27465.1.
RefSeqi NP_081488.1. NM_027212.2.
UniGenei Mm.195562.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 213682. 2 interactions.
IntActi Q9CQI9. 7 interactions.
MINTi MINT-217060.
STRINGi 10090.ENSMUSP00000042204.

PTM databases

PhosphoSitei Q9CQI9.

Proteomic databases

PaxDbi Q9CQI9.
PRIDEi Q9CQI9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000037115 ; ENSMUSP00000042204 ; ENSMUSG00000038622 .
GeneIDi 69790.
KEGGi mmu:69790.
UCSCi uc007vrg.1. mouse.

Organism-specific databases

CTDi 90390.
MGIi MGI:1917040. Med30.

Phylogenomic databases

eggNOGi NOG87821.
GeneTreei ENSGT00390000010887.
HOGENOMi HOG000006885.
HOVERGENi HBG107279.
InParanoidi Q9CQI9.
KOi K15143.
OMAi GSKHDDR.
OrthoDBi EOG7C5MB0.
PhylomeDBi Q9CQI9.
TreeFami TF324588.

Enzyme and pathway databases

Reactomei REACT_198602. PPARA activates gene expression.
REACT_206529. Generic Transcription Pathway.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

NextBioi 330352.
PROi Q9CQI9.
SOURCEi Search...

Gene expression databases

Bgeei Q9CQI9.
CleanExi MM_MED30.
Genevestigatori Q9CQI9.

Family and domain databases

InterProi IPR021019. Mediator_Med30_met.
[Graphical view ]
Pfami PF11315. Med30. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and DBA/2.
    Tissue: Liver and Pancreas.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiMED30_MOUSE
AccessioniPrimary (citable) accession number: Q9CQI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi