ID   RAB5A_MOUSE             Reviewed;         215 AA.
AC   Q9CQD1; Q9DCN5;
DT   20-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   02-MAR-2010, entry version 85.
DE   RecName: Full=Ras-related protein Rab-5A;
GN   Name=Rab5a;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J;
RC   TISSUE=Embryonic stem cell, Head, and Small intestine;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 23-33; 82-110 AND 184-195, AND MASS SPECTROMETRY.
RC   STRAIN=C57BL/6; TISSUE=Brain;
RA   Lubec G., Kang S.U.;
RL   Submitted (APR-2007) to UniProtKB.
RN   [4]
RP   INTERACTION WITH SGSM1 AND SGSM3.
RX   PubMed=17509819; DOI=10.1016/j.ygeno.2007.03.013;
RA   Yang H., Sasaki T., Minoshima S., Shimizu N.;
RT   "Identification of three novel proteins (SGSM1, 2, 3) which modulate
RT   small G protein (RAP and RAB)-mediated signaling pathway.";
RL   Genomics 90:249-260(2007).
CC   -!- FUNCTION: Required for the fusion of plasma membranes and early
CC       endosomes.
CC   -!- ENZYME REGULATION: Regulated by guanine nucleotide exchange
CC       factors (GEFs) which promote the exchange of bound GDP for free
CC       GTP.
CC   -!- SUBUNIT: Binds EEA1. Interacts with ALS2CL, UNC84B, ZFYVE20 and
CC       RUFY1. Interacts with RIN1 and GAPVD1, which regulate its pathway,
CC       probably by acting as a GEF (By similarity). Interacts with SGSM1
CC       and SGSM3.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic
CC       side (By similarity). Early endosome membrane; Lipid-anchor (By
CC       similarity). Melanosome (By similarity).
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
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DR   EMBL; AK002631; BAB22245.1; -; mRNA.
DR   EMBL; AK008198; BAB25527.1; -; mRNA.
DR   EMBL; AK010495; BAB26985.1; -; mRNA.
DR   EMBL; AK081999; BAC38391.1; -; mRNA.
DR   EMBL; BC004842; AAH04842.1; -; mRNA.
DR   EMBL; BC034370; AAH34370.1; -; mRNA.
DR   IPI; IPI00132410; -.
DR   RefSeq; NP_080163.1; -.
DR   UniGene; Mm.329123; -.
DR   SMR; Q9CQD1; 15-184.
DR   IntAct; Q9CQD1; 1.
DR   STRING; Q9CQD1; -.
DR   PhosphoSite; Q9CQD1; -.
DR   PRIDE; Q9CQD1; -.
DR   Ensembl; ENSMUST00000017975; ENSMUSP00000017975; ENSMUSG00000017831; Mus musculus.
DR   GeneID; 271457; -.
DR   KEGG; mmu:271457; -.
DR   UCSC; uc008czn.1; mouse.
DR   CTD; 271457; -.
DR   MGI; MGI:105926; Rab5a.
DR   HOGENOM; HBG745225; -.
DR   HOVERGEN; HBG009351; -.
DR   InParanoid; Q9CQD1; -.
DR   OMA; PTRNQCC; -.
DR   OrthoDB; EOG9M3CV3; -.
DR   PhylomeDB; Q9CQD1; -.
DR   NextBio; 393462; -.
DR   ArrayExpress; Q9CQD1; -.
DR   Bgee; Q9CQD1; -.
DR   CleanEx; MM_RAB5A; -.
DR   Genevestigator; Q9CQD1; -.
DR   GermOnline; ENSMUSG00000017831; Mus musculus.
DR   GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030139; C:endocytic vesicle; IDA:MGI.
DR   GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0045121; C:membrane raft; IDA:MGI.
DR   GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR   GO; GO:0001726; C:ruffle; IDA:MGI.
DR   GO; GO:0019003; F:GDP binding; ISS:UniProtKB.
DR   GO; GO:0005525; F:GTP binding; ISS:UniProtKB.
DR   GO; GO:0003924; F:GTPase activity; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   InterPro; IPR003579; GTPase_Rab.
DR   InterPro; IPR015599; Rab5-like.
DR   InterPro; IPR013753; Ras.
DR   InterPro; IPR001806; Ras_GTPase.
DR   InterPro; IPR005225; Small_GTP_bd.
DR   InterPro; IPR020851; Small_GTPase.
DR   PANTHER; PTHR11708:SF254; Rab5_like; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PRINTS; PR00449; RASTRNSFRMNG.
DR   SMART; SM00175; RAB; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51419; RAB; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cell membrane; Direct protein sequencing; Endosome;
KW   GTP-binding; Lipoprotein; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Prenylation; Protein transport; Transport.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    215       Ras-related protein Rab-5A.
FT                                /FTId=PRO_0000121106.
FT   NP_BIND      27     35       GTP (By similarity).
FT   NP_BIND      75     79       GTP (By similarity).
FT   NP_BIND     133    136       GTP (By similarity).
FT   NP_BIND     163    165       GTP (By similarity).
FT   MOTIF        49     57       Effector region (By similarity).
FT   MOD_RES       2      2       N-acetylalanine (By similarity).
FT   MOD_RES     202    202       Phosphothreonine (By similarity).
FT   LIPID       212    212       S-geranylgeranyl cysteine (By
FT                                similarity).
FT   LIPID       213    213       S-geranylgeranyl cysteine (By
FT                                similarity).
FT   CONFLICT    119    119       Q -> R (in Ref. 1; BAB22245).
SQ   SEQUENCE   215 AA;  23599 MW;  9CDE2FD9B37C261A CRC64;
     MANRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE STIGAAFLTQ
     TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY DITNEESFAR AKNWVKELQR
     QASPNIVIAL SGNKADLANK RAVDFQEAQS YADDNSLLFM ETSAKTSMNV NEIFMAIAKK
     LPKNEPQNPG ANSARGRGVD LTEPAQPARS QCCSN
//