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Protein

Ras-related protein Rab-5A

Gene

Rab5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (By similarity). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (PubMed:18425118).By similarity1 Publication

Enzyme regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35GTPBy similarity9
Nucleotide bindingi46 – 52GTPBy similarity7
Nucleotide bindingi75 – 79GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4
Nucleotide bindingi163 – 165GTPBy similarity3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB
  • guanyl nucleotide binding Source: MGI

GO - Biological processi

Keywordsi

Biological processEndocytosis, Phagocytosis, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660499 Synthesis of PIPs at the plasma membrane
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-8873719 RAB geranylgeranylation
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-5A
Gene namesi
Name:Rab5a
Synonyms:nnyRab5a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:105926 Rab5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi34S → N: Inhibits endosome localization. Disrupts interaction of RAB5A and OCRL with INPP5F. Prevents its localization to apoptotic cell corpse-containing early phagosomes. Maturation of apoptotic cell-containing phagosome into acidic phagosomes is decreased. Interacts with PIK3C3. 2 Publications1
Mutagenesisi79Q → L: Induces the formation of larger endosomes. No effect on interaction with INPP5F. Prevents interaction with DYN2 without affecting the interaction with PIK3C3. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211061 – 215Ras-related protein Rab-5AAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi212S-geranylgeranyl cysteineBy similarity1
Lipidationi213S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

EPDiQ9CQD1
MaxQBiQ9CQD1
PaxDbiQ9CQD1
PeptideAtlasiQ9CQD1
PRIDEiQ9CQD1

PTM databases

iPTMnetiQ9CQD1
PhosphoSitePlusiQ9CQD1
SwissPalmiQ9CQD1

Expressioni

Gene expression databases

BgeeiENSMUSG00000017831
CleanExiMM_RAB5A
GenevisibleiQ9CQD1 MM

Interactioni

Subunit structurei

Interacts with GDI1; this promotes dissociation from membranes. Interacts with EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with RINL. Interacts with ALS2CL, SUN2, ZFYVE20 and RUFY1. Interacts with RABEP1; one RABEP1 homodimer binds two RAB5A chains, but at opposite sides of the dimer (By similarity). Interacts with SGSM1 and SGSM3 (PubMed:17509819). Interacts with PIK3CB (PubMed:21059846). Interacts with OCRL and INPP5F (PubMed:25869668). May be a component of a complex composed of RAB5A, DYN2 and PIK3C3 (PubMed:18425118). Does not interact with BLOC-3 complex (heterodimer of HPS1 and HPS4). Interacts with CLN5 (By similarity).By similarity1 Publication3 Publications

Protein-protein interaction databases

BioGridi234840, 6 interactors
IntActiQ9CQD1, 11 interactors
MINTiQ9CQD1
STRINGi10090.ENSMUSP00000017975

Structurei

3D structure databases

ProteinModelPortaliQ9CQD1
SMRiQ9CQD1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 57Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0092 Eukaryota
ENOG410YCCP LUCA
GeneTreeiENSGT00760000119101
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiQ9CQD1
KOiK07887
OMAiCLLFMET
OrthoDBiEOG091G0HKN
PhylomeDBiQ9CQD1
TreeFamiTF300199

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CQD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE
60 70 80 90 100
STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY
110 120 130 140 150
DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS
160 170 180 190 200
YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD
210
LTEPAQPARS QCCSN
Length:215
Mass (Da):23,599
Last modified:June 1, 2001 - v1
Checksum:i9CDE2FD9B37C261A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13N → D in BAE29485 (PubMed:16141072).Curated1
Sequence conflicti119Q → R in BAB22245 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB232593 mRNA Translation: BAF02855.1
AK002631 mRNA Translation: BAB22245.1
AK008198 mRNA Translation: BAB25527.1
AK010495 mRNA Translation: BAB26985.1
AK081999 mRNA Translation: BAC38391.1
AK150296 mRNA Translation: BAE29448.1
AK150346 mRNA Translation: BAE29485.1
AK150405 mRNA Translation: BAE29531.1
AK151066 mRNA Translation: BAE30082.1
AK153131 mRNA Translation: BAE31744.1
AK159706 mRNA Translation: BAE35305.1
CH466559 Genomic DNA Translation: EDL23663.1
BC004842 mRNA Translation: AAH04842.1
BC034370 mRNA Translation: AAH34370.1
BC096481 mRNA Translation: AAH96481.1
CCDSiCCDS28879.1
RefSeqiNP_080163.1, NM_025887.4
UniGeneiMm.329123

Genome annotation databases

EnsembliENSMUST00000017975; ENSMUSP00000017975; ENSMUSG00000017831
GeneIDi271457
KEGGimmu:271457
UCSCiuc008czn.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRAB5A_MOUSE
AccessioniPrimary (citable) accession number: Q9CQD1
Secondary accession number(s): Q3UCX7, Q4VAA1, Q9DCN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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