Q9CQC9 (SAR1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP-binding protein SAR1b

Gene names

Name:	Sar1b
Synonyms:	Sara1b, Sara2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.
Subunit structure	Homodimer. Part of the COPII coat complex. Binds to the cytoplasmic tails of target proteins in the endoplasmic reticulum By similarity. Binds PREB. Ref.3
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein. Note: Associated with the endoplasmic reticulum and Golgi stacks, in particular in the juxta-nuclear Golgi region. Ref.3
Sequence similarities	Belongs to the small GTPase superfamily. SAR1 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	GTP-binding protein SAR1b		PRO_0000206262
Regions
	Nucleotide binding	32 – 39	8	GTP By similarity
	Nucleotide binding	75 – 78	4	GTP By similarity
	Nucleotide binding	134 – 137	4	GTP By similarity
Sites
	Metal binding	34	1	Magnesium By similarity
	Metal binding	75	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CQC9 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 9D0173C53FCD7A6B	FASTA	198	22,382
	10 20 30 40 50 60 MSFIFDWIYS GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL GQHVPTLHPT 70 80 90 100 110 120 SEELTIAGMT FTTFDLGGHV QARRVWKNYL PAINGIVFLV DCADHERLLE SKEELDSLMT 130 140 150 160 170 180 DETIANVPIL ILGNKIDRPE AISEERLREM FGLYGQTTGK GSVSLKELNA RPLEVFMCSV 190 LKRQGYGEGF RWMAQYID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow, Hippocampus, Kidney and Tongue.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"The mammalian guanine nucleotide exchange factor mSec12 is essential for activation of the Sar1 GTPase directing endoplasmic reticulum export." Weissman J.T., Plutner H., Balch W.E. Traffic 2:465-475(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PREB, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK002327 mRNA. Translation: BAB22015.1. AK010187 mRNA. Translation: BAB26755.1. AK013559 mRNA. Translation: BAB28905.1. AK150906 mRNA. Translation: BAE29948.1. BC082550 mRNA. Translation: AAH82550.1.
IPI	IPI00132397.
RefSeq	NP_079811.1. NM_025535.2.
UniGene	Mm.196592.
3D structure databases
ProteinModelPortal	Q9CQC9.
SMR	Q9CQC9. Positions 13-198.
ModBase	Search...
PTM databases
PhosphoSite	Q9CQC9.
Proteomic databases
PaxDb	Q9CQC9.
PRIDE	Q9CQC9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020653; ENSMUSP00000020653; ENSMUSG00000020386.
GeneID	66397.
KEGG	mmu:66397.
Organism-specific databases
CTD	51128.
MGI	MGI:1913647. Sar1b.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00550000074696.
HOGENOM	HOG000163690.
HOVERGEN	HBG104997.
InParanoid	Q9CQC9.
KO	K07953.
OMA	SKEELDX.
OrthoDB	EOG4SJ5FT.
Gene expression databases
ArrayExpress	Q9CQC9.
Bgee	Q9CQC9.
CleanEx	MM_SAR1B.
Genevestigator	Q9CQC9.
GermOnline	ENSMUSG00000020386. Mus musculus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR006689. Small_GTPase_ARF/SAR. IPR006687. Small_GTPase_SAR1. [Graphical view]
PANTHER	PTHR11711:SF12. PTHR11711:SF12. 1 hit.
Pfam	PF00025. Arf. 1 hit. [Graphical view]
PRINTS	PR00328. SAR1GTPBP.
SMART	SM00178. SAR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51422. SAR1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	321555.
SOURCE	Search...

Entry information

Entry name

SAR1B_MOUSE

Accession

Primary (citable) accession number: Q9CQC9
Secondary accession number(s): Q3UBL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 9, 2003
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program