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Protein

CDGSH iron-sulfur domain-containing protein 2

Gene

Cisd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of autophagy that contributes to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca2+ stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy (By similarity).By similarity1 Publication

Cofactori

[2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity

Redox potential

E is 0 +/- 10 mV for 2Fe-2S at pH 7.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi101Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi110Iron-sulfur (2Fe-2S)By similarity1
Metal bindingi114Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • mitophagy Source: UniProtKB
  • multicellular organism aging Source: UniProtKB
  • regulation of autophagy Source: MGI
Complete GO annotation...

Keywords - Biological processi

Autophagy

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CDGSH iron-sulfur domain-containing protein 2
Alternative name(s):
MitoNEET-related 1 protein
Short name:
Miner1
Nervous system overexpressed protein 70
Gene namesi
Name:Cisd2
Synonyms:Cdgsh2, Noxp70, Zcd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1914256. Cisd2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 37LumenalSequence analysisAdd BLAST36
Transmembranei38 – 60HelicalSequence analysisAdd BLAST23
Topological domaini61 – 135CytoplasmicSequence analysisAdd BLAST75

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Premature aging associated with a shortened life span, probably caused by mitochondrial degeneration and autophagy. 8 week old mice begin to acquire a set of aged appearance phenomena remarkably similar to those of premature aging syndrome, including prominent eyes and protruding ears. Ocular abnormalities are observed: mice develop opaque eyes and blindness, which is accompanied by cornea damage at 20 week old. The opacity of the cornea is due to debris deposition in the scar tissue outside the cornea. In addition, corneal neovascularization is observed, possibly impairing vision. An early depigmentation in the fur at around 48 week old is also observed; furthermore, hair follicle atrophy and a decreased hair density is detected. A decrease in the hair regrowth rate is also observed. Additionally, the skin of 48-week-old mice exhibits a phenotype with a noticeably thickened dermis, an expanded surface, and a significant decrease in subcutaneous adipose tissue and muscle. The trabeculae of the femur are noticeably thinner and dual energy X-ray absorptiometer (DEXA) detects a decrease in femur density after 8 week old. Mice also display a significant lordokyphosis phenotype after 12 week old; leading to a decrease in mean thoracic volume and thence pulmonary function abnormalities. Muscle degeneration is detectable at 3 week old with a progressive degeneration of muscle fibers and the magnitude of the degeneration exacerbated with age.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003160062 – 135CDGSH iron-sulfur domain-containing protein 2Add BLAST134

Proteomic databases

EPDiQ9CQB5.
MaxQBiQ9CQB5.
PaxDbiQ9CQB5.
PeptideAtlasiQ9CQB5.
PRIDEiQ9CQB5.

PTM databases

iPTMnetiQ9CQB5.
PhosphoSitePlusiQ9CQB5.
SwissPalmiQ9CQB5.

Expressioni

Tissue specificityi

Brain.1 Publication

Inductioni

Expression decreases in an age-dependent manner.1 Publication

Gene expression databases

BgeeiENSMUSG00000028165.
CleanExiMM_CISD2.
ExpressionAtlasiQ9CQB5. baseline and differential.
GenevisibleiQ9CQB5. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with BCL2; the interaction is direct and disrupted by BIK interaction with BCL2. Interacts with BCL2L1. Interacts with ITPR1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9CQB5. 3 interactors.
MINTiMINT-4126498.
STRINGi10090.ENSMUSP00000029815.

Structurei

3D structure databases

ProteinModelPortaliQ9CQB5.
SMRiQ9CQB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CISD protein family. CISD2 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3461. Eukaryota.
ENOG41122II. LUCA.
GeneTreeiENSGT00390000001233.
HOGENOMiHOG000242301.
HOVERGENiHBG052444.
InParanoidiQ9CQB5.
OMAiHGEHNKL.
OrthoDBiEOG091G0XB4.
PhylomeDBiQ9CQB5.
TreeFamiTF324661.

Family and domain databases

InterProiIPR018967. FeS-contain_CDGSH-typ.
IPR019610. FeS-contain_mitoNEET_N.
[Graphical view]
PfamiPF10660. MitoNEET_N. 1 hit.
PF09360. zf-CDGSH. 1 hit.
[Graphical view]
SMARTiSM00704. ZnF_CDGSH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLDSVARIV KVQLPAYLKQ LPVPDSITGF ARLTVSDWLR LLPFLGVLAL
60 70 80 90 100
LGYLAVRPFF PKKKQQKDSL INLKIQKENP KVVNEINIED LCLTKAAYCR
110 120 130
CWRSKTFPAC DGSHNKHNEL TGDNVGPLIL KKKEV
Length:135
Mass (Da):15,242
Last modified:June 1, 2001 - v1
Checksum:iA46C87D9C6F73EF0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61P → A in BAB23237 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM162549 mRNA. Translation: CAJ44265.1.
AK003486 mRNA. Translation: BAB22814.1.
AK004257 mRNA. Translation: BAB23237.1.
AK005184 mRNA. Translation: BAB23868.1.
BC058279 mRNA. Translation: AAH58279.1.
CCDSiCCDS17856.1.
RefSeqiNP_080178.1. NM_025902.3.
XP_006501981.1. XM_006501918.3.
UniGeneiMm.269993.

Genome annotation databases

EnsembliENSMUST00000029815; ENSMUSP00000029815; ENSMUSG00000028165.
GeneIDi67006.
KEGGimmu:67006.
UCSCiuc008rlk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM162549 mRNA. Translation: CAJ44265.1.
AK003486 mRNA. Translation: BAB22814.1.
AK004257 mRNA. Translation: BAB23237.1.
AK005184 mRNA. Translation: BAB23868.1.
BC058279 mRNA. Translation: AAH58279.1.
CCDSiCCDS17856.1.
RefSeqiNP_080178.1. NM_025902.3.
XP_006501981.1. XM_006501918.3.
UniGeneiMm.269993.

3D structure databases

ProteinModelPortaliQ9CQB5.
SMRiQ9CQB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CQB5. 3 interactors.
MINTiMINT-4126498.
STRINGi10090.ENSMUSP00000029815.

PTM databases

iPTMnetiQ9CQB5.
PhosphoSitePlusiQ9CQB5.
SwissPalmiQ9CQB5.

Proteomic databases

EPDiQ9CQB5.
MaxQBiQ9CQB5.
PaxDbiQ9CQB5.
PeptideAtlasiQ9CQB5.
PRIDEiQ9CQB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029815; ENSMUSP00000029815; ENSMUSG00000028165.
GeneIDi67006.
KEGGimmu:67006.
UCSCiuc008rlk.2. mouse.

Organism-specific databases

CTDi493856.
MGIiMGI:1914256. Cisd2.

Phylogenomic databases

eggNOGiKOG3461. Eukaryota.
ENOG41122II. LUCA.
GeneTreeiENSGT00390000001233.
HOGENOMiHOG000242301.
HOVERGENiHBG052444.
InParanoidiQ9CQB5.
OMAiHGEHNKL.
OrthoDBiEOG091G0XB4.
PhylomeDBiQ9CQB5.
TreeFamiTF324661.

Miscellaneous databases

PROiQ9CQB5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028165.
CleanExiMM_CISD2.
ExpressionAtlasiQ9CQB5. baseline and differential.
GenevisibleiQ9CQB5. MM.

Family and domain databases

InterProiIPR018967. FeS-contain_CDGSH-typ.
IPR019610. FeS-contain_mitoNEET_N.
[Graphical view]
PfamiPF10660. MitoNEET_N. 1 hit.
PF09360. zf-CDGSH. 1 hit.
[Graphical view]
SMARTiSM00704. ZnF_CDGSH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCISD2_MOUSE
AccessioniPrimary (citable) accession number: Q9CQB5
Secondary accession number(s): Q9D0Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.