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Protein

Mediator of RNA polymerase II transcription subunit 4

Gene

Med4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 4
Alternative name(s):
Mediator complex subunit 4
Gene namesi
Name:Med4
Synonyms:Vdrip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1914631. Med4.

Subcellular locationi

GO - Cellular componenti

  • core mediator complex Source: GO_Central
  • mediator complex Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 270269Mediator of RNA polymerase II transcription subunit 4PRO_0000096384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei32 – 321PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CQA5.
MaxQBiQ9CQA5.
PaxDbiQ9CQA5.
PeptideAtlasiQ9CQA5.
PRIDEiQ9CQA5.

PTM databases

iPTMnetiQ9CQA5.
PhosphoSiteiQ9CQA5.

Expressioni

Gene expression databases

BgeeiQ9CQA5.
GenevisibleiQ9CQA5. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212147. 2 interactions.
IntActiQ9CQA5. 5 interactions.
MINTiMINT-4124821.
STRINGi10090.ENSMUSP00000022705.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili26 – 131106Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi262 – 2698Poly-Ser

Sequence similaritiesi

Belongs to the Mediator complex subunit 4 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4552. Eukaryota.
ENOG410XQ30. LUCA.
GeneTreeiENSGT00390000012063.
HOGENOMiHOG000043883.
HOVERGENiHBG052450.
InParanoidiQ9CQA5.
KOiK15146.
OMAiGLEPPGH.
OrthoDBiEOG735405.
PhylomeDBiQ9CQA5.
TreeFamiTF324421.

Family and domain databases

InterProiIPR019258. Mediator_Med4.
[Graphical view]
PfamiPF10018. Med4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CQA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASSSGEKE KERMGGVSGM AGLGSTRERL LSALEDLEVL SRELIEMLAI
60 70 80 90 100
SRNQKLLQLE EENQVLELLI HRDGDFQELM KLALNQGKVH HEMQALEKEV
110 120 130 140 150
EKRDSDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK
160 170 180 190 200
YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTSGVNG
210 220 230 240 250
HLPGDALAAG RLPDVLAPQY PWQSNDMSVN MLPPNHSSDF LLEPPGHNKE
260 270
NEDDVEVMST DSSSSSSDSD
Length:270
Mass (Da):29,781
Last modified:June 1, 2001 - v1
Checksum:iA827D43766AAAD52
GO
Isoform 2 (identifier: Q9CQA5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     171-177: DPRRPYP → NRASYIR
     178-270: Missing.

Note: No experimental confirmation available.
Show »
Length:177
Mass (Da):19,868
Checksum:iBD12B395306DDDB9
GO

Sequence cautioni

The sequence BAE26554.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei171 – 1777DPRRPYP → NRASYIR in isoform 2. 1 PublicationVSP_027918
Alternative sequencei178 – 27093Missing in isoform 2. 1 PublicationVSP_027919Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010270 mRNA. Translation: BAB26809.1.
AK010686 mRNA. Translation: BAB27118.1.
AK078288 mRNA. Translation: BAC37206.1.
AK145636 mRNA. Translation: BAE26554.1. Different initiation.
BC024950 mRNA. Translation: AAH24950.1.
CCDSiCCDS49538.1. [Q9CQA5-1]
RefSeqiNP_080395.1. NM_026119.3. [Q9CQA5-1]
UniGeneiMm.282888.

Genome annotation databases

EnsembliENSMUST00000022705; ENSMUSP00000022705; ENSMUSG00000022109. [Q9CQA5-1]
GeneIDi67381.
KEGGimmu:67381.
UCSCiuc007upu.1. mouse. [Q9CQA5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010270 mRNA. Translation: BAB26809.1.
AK010686 mRNA. Translation: BAB27118.1.
AK078288 mRNA. Translation: BAC37206.1.
AK145636 mRNA. Translation: BAE26554.1. Different initiation.
BC024950 mRNA. Translation: AAH24950.1.
CCDSiCCDS49538.1. [Q9CQA5-1]
RefSeqiNP_080395.1. NM_026119.3. [Q9CQA5-1]
UniGeneiMm.282888.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212147. 2 interactions.
IntActiQ9CQA5. 5 interactions.
MINTiMINT-4124821.
STRINGi10090.ENSMUSP00000022705.

PTM databases

iPTMnetiQ9CQA5.
PhosphoSiteiQ9CQA5.

Proteomic databases

EPDiQ9CQA5.
MaxQBiQ9CQA5.
PaxDbiQ9CQA5.
PeptideAtlasiQ9CQA5.
PRIDEiQ9CQA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022705; ENSMUSP00000022705; ENSMUSG00000022109. [Q9CQA5-1]
GeneIDi67381.
KEGGimmu:67381.
UCSCiuc007upu.1. mouse. [Q9CQA5-1]

Organism-specific databases

CTDi29079.
MGIiMGI:1914631. Med4.

Phylogenomic databases

eggNOGiKOG4552. Eukaryota.
ENOG410XQ30. LUCA.
GeneTreeiENSGT00390000012063.
HOGENOMiHOG000043883.
HOVERGENiHBG052450.
InParanoidiQ9CQA5.
KOiK15146.
OMAiGLEPPGH.
OrthoDBiEOG735405.
PhylomeDBiQ9CQA5.
TreeFamiTF324421.

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

PROiQ9CQA5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CQA5.
GenevisibleiQ9CQA5. MM.

Family and domain databases

InterProiIPR019258. Mediator_Med4.
[Graphical view]
PfamiPF10018. Med4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Olfactory bulb.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiMED4_MOUSE
AccessioniPrimary (citable) accession number: Q9CQA5
Secondary accession number(s): Q3UL96, Q8BVG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.