ID   CENPM_MOUSE             Reviewed;         180 AA.
AC   Q9CQA0; Q3THN3; Q91VS6;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   24-JAN-2024, entry version 130.
DE   RecName: Full=Centromere protein M;
DE            Short=CENP-M;
DE   AltName: Full=Proliferation-associated nuclear element protein 1;
GN   Name=Cenpm; Synonyms=Pane1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND SUBCELLULAR LOCATION.
RC   STRAIN=C57BL/6J; TISSUE=Lactating mammary gland;
RX   PubMed=12881717; DOI=10.1038/sj.onc.1206596;
RA   Renou J.-P., Bierie B., Miyoshi K., Cui Y., Djiane J., Reichenstein M.,
RA   Shani M., Hennighausen L.;
RT   "Identification of genes differentially expressed in mouse mammary
RT   epithelium transformed by an activated beta-catenin.";
RL   Oncogene 22:4594-4610(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=C57BL/6J, and DBA/2J; TISSUE=Embryonic liver;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the CENPA-NAC (nucleosome-associated) complex, a
CC       complex that plays a central role in assembly of kinetochore proteins,
CC       mitotic progression and chromosome segregation. The CENPA-NAC complex
CC       recruits the CENPA-CAD (nucleosome distal) complex and may be involved
CC       in incorporation of newly synthesized CENPA into centromeres (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the CENPA-NAC complex, at least composed of
CC       CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC
CC       complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK,
CC       CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12881717}. Cytoplasm
CC       {ECO:0000269|PubMed:12881717}. Chromosome, centromere, kinetochore
CC       {ECO:0000250}. Note=Nuclear in non-confluent cells and cytoplasmic in
CC       confluent or dividing cells. Localizes in the kinetochore domain of
CC       centromeres (By similarity). {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9CQA0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9CQA0-2; Sequence=VSP_020440;
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY243536; AAO62567.1; -; mRNA.
DR   EMBL; AY243537; AAO62568.1; -; mRNA.
DR   EMBL; AK010885; BAB27246.1; -; mRNA.
DR   EMBL; AK011191; BAB27456.1; -; mRNA.
DR   EMBL; AK011227; BAB27479.1; -; mRNA.
DR   EMBL; AK011464; BAB27635.1; -; mRNA.
DR   EMBL; AK011755; BAB27823.1; -; mRNA.
DR   EMBL; AK168203; BAE40163.1; -; mRNA.
DR   EMBL; AK017679; BAB30869.1; -; mRNA.
DR   EMBL; BC009160; AAH09160.1; -; mRNA.
DR   CCDS; CCDS27685.1; -. [Q9CQA0-1]
DR   CCDS; CCDS37156.1; -. [Q9CQA0-2]
DR   RefSeq; NP_001073627.1; NM_001080158.1.
DR   RefSeq; NP_079915.1; NM_025639.4. [Q9CQA0-1]
DR   RefSeq; NP_840000.2; NM_178269.3. [Q9CQA0-2]
DR   AlphaFoldDB; Q9CQA0; -.
DR   SMR; Q9CQA0; -.
DR   ComplexPortal; CPX-5704; Kinetochore CCAN complex.
DR   IntAct; Q9CQA0; 1.
DR   MINT; Q9CQA0; -.
DR   STRING; 10090.ENSMUSP00000086560; -.
DR   PhosphoSitePlus; Q9CQA0; -.
DR   EPD; Q9CQA0; -.
DR   MaxQB; Q9CQA0; -.
DR   PaxDb; 10090-ENSMUSP00000086560; -.
DR   ProteomicsDB; 280063; -. [Q9CQA0-1]
DR   ProteomicsDB; 280064; -. [Q9CQA0-2]
DR   Pumba; Q9CQA0; -.
DR   Antibodypedia; 27112; 162 antibodies from 25 providers.
DR   DNASU; 66570; -.
DR   Ensembl; ENSMUST00000089155.6; ENSMUSP00000086558.5; ENSMUSG00000068101.12. [Q9CQA0-2]
DR   Ensembl; ENSMUST00000089157.11; ENSMUSP00000086560.4; ENSMUSG00000068101.12. [Q9CQA0-1]
DR   GeneID; 66570; -.
DR   KEGG; mmu:66570; -.
DR   UCSC; uc007wym.1; mouse. [Q9CQA0-1]
DR   UCSC; uc007wyo.1; mouse. [Q9CQA0-2]
DR   AGR; MGI:1913820; -.
DR   CTD; 79019; -.
DR   MGI; MGI:1913820; Cenpm.
DR   VEuPathDB; HostDB:ENSMUSG00000068101; -.
DR   eggNOG; ENOG502S17M; Eukaryota.
DR   GeneTree; ENSGT00390000017504; -.
DR   HOGENOM; CLU_127179_0_0_1; -.
DR   InParanoid; Q9CQA0; -.
DR   OMA; CTLPLDI; -.
DR   OrthoDB; 5385872at2759; -.
DR   PhylomeDB; Q9CQA0; -.
DR   TreeFam; TF328778; -.
DR   Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR   Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-MMU-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-MMU-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR   Reactome; R-MMU-68877; Mitotic Prometaphase.
DR   Reactome; R-MMU-9648025; EML4 and NUDC in mitotic spindle formation.
DR   BioGRID-ORCS; 66570; 34 hits in 80 CRISPR screens.
DR   ChiTaRS; Cenpm; mouse.
DR   PRO; PR:Q9CQA0; -.
DR   Proteomes; UP000000589; Chromosome 15.
DR   RNAct; Q9CQA0; Protein.
DR   Bgee; ENSMUSG00000068101; Expressed in blastoderm cell in morula and 118 other cell types or tissues.
DR   ExpressionAtlas; Q9CQA0; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000939; C:inner kinetochore; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; NAS:ComplexPortal.
DR   GO; GO:0007059; P:chromosome segregation; NAS:ComplexPortal.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR020987; Centromere_Cenp-M.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR34436; CENTROMERE PROTEIN M; 1.
DR   PANTHER; PTHR34436:SF1; CENTROMERE PROTEIN M; 1.
DR   Pfam; PF11111; CENP-M; 1.
DR   Genevisible; Q9CQA0; MM.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Centromere; Chromosome; Cytoplasm; Kinetochore;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..180
FT                   /note="Centromere protein M"
FT                   /id="PRO_0000058225"
FT   VAR_SEQ         104..180
FT                   /note="AGQESHCSVHQNTVIKLAHTYRSPLLLCDLQVESFRAAMARRLVRILQICAG
FT                   HVPGVSALNLMSLLRSPENPPSKEL -> GGKLSCSHGAASGAHSADLRWPCARRLRAE
FT                   PDVLAAEPREPPIQGAVSITALHTFAASSKSSHCW (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_020440"
FT   CONFLICT        13
FT                   /note="L -> M (in Ref. 2; BAE40163)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        47
FT                   /note="H -> N (in Ref. 2; BAE40163)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        129
FT                   /note="L -> F (in Ref. 3; AAH09160)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        134
FT                   /note="Q -> QK (in Ref. 3; AAH09160)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   180 AA;  20039 MW;  AA6396B75269346C CRC64;
     MSVLRSMDKL PDLNRATVLL VSTEDALLQQ LAESMLKDDC ASELRVHLAN SLPLPSNVNR
     PRIDLIVFVI NLHSKYSLQK VEEFLQHVDS SFFLGKVCFL VTGAGQESHC SVHQNTVIKL
     AHTYRSPLLL CDLQVESFRA AMARRLVRIL QICAGHVPGV SALNLMSLLR SPENPPSKEL
//