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Protein

Centromere protein R

Gene

Itgb3bp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription coregulator that can have both coactivator and corepressor functions. Involved in the coactivation of nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent fashion. In contrast, it does not coactivate nuclear receptors for retinoic acid, vitamin D, progesterone receptor, nor glucocorticoid. Acts as a coactivator for estrogen receptor alpha. Acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. May also act as an inhibitor of cyclin A-associated kinase. Also acts a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-205043. NRIF signals cell death from the nucleus.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-MMU-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein R
Short name:
CENP-R
Alternative name(s):
Nuclear receptor-interacting factor 3
Gene namesi
Name:Itgb3bp
Synonyms:Cenpr, Nrif3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914983. Itgb3bp.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579501 – 176Centromere protein RAdd BLAST176

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineBy similarity1
Modified residuei71PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9CQ82.
PeptideAtlasiQ9CQ82.
PRIDEiQ9CQ82.

PTM databases

iPTMnetiQ9CQ82.
PhosphoSitePlusiQ9CQ82.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028549.
CleanExiMM_ITGB3BP.
GenevisibleiQ9CQ82. MM.

Interactioni

Subunit structurei

Homodimer; mediated by the coiled coil domain. Interacts with CCNA2 and MTA1. Interacts with NFKB1 NF-kappa-B subunit. Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117153.

Structurei

3D structure databases

ProteinModelPortaliQ9CQ82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 50DD1Add BLAST31

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili82 – 112Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi63 – 66Nuclear localization signalBy similarity4
Motifi171 – 175LXXIL motif5

Domaini

The DD1 domain (also called RepD1 domain) mediates the corepressor function and is essential in the triggering of apoptosis.By similarity
Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL) motif, which is essential for the association with nuclear receptors.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410J05S. Eukaryota.
ENOG410Z43R. LUCA.
GeneTreeiENSGT00390000004336.
HOGENOMiHOG000049271.
HOVERGENiHBG080553.
InParanoidiQ9CQ82.
KOiK11510.
OMAiCHLDSYE.
OrthoDBiEOG091G180Q.
PhylomeDBiQ9CQ82.
TreeFamiTF336291.

Family and domain databases

InterProiIPR009601. CENP-R.
[Graphical view]
PANTHERiPTHR15581. PTHR15581. 1 hit.
PfamiPF06729. CENP-R. 1 hit.
[Graphical view]
PIRSFiPIRSF011860. NRIF3_coact_rcpt. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CQ82-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVKRSLKLD DQFEKNSFSP SKIVRKKSIT AYSPTTGTYQ LSPFSSPATP
60 70 80 90 100
KEQEHRNGPS NETRKRSNLS SPVRQESTVK DRDGFMVLLS KIEISSEKTM
110 120 130 140 150
EIMKNLSSIQ ALEGNRQLED LIGVSLVPCS LKSEARKTKE LMTKVIKQKL
160 170
FEKKKSRITP KDHHLDSFEF LKAILN
Length:176
Mass (Da):20,026
Last modified:June 1, 2001 - v1
Checksum:iB55F0C100CAE5973
GO
Isoform 2 (identifier: Q9CQ82-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.
     78-84: TVKDRDG → MISLKKI

Note: No experimental confirmation available.
Show »
Length:99
Mass (Da):11,390
Checksum:i112AA4C302DED413
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7L → V in BAE38732 (PubMed:16141072).Curated1
Sequence conflicti50P → S in BAE38732 (PubMed:16141072).Curated1
Sequence conflicti82R → S in BAE38732 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0204531 – 77Missing in isoform 2. 1 PublicationAdd BLAST77
Alternative sequenceiVSP_02045478 – 84TVKDRDG → MISLKKI in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005779 mRNA. Translation: BAB24235.1.
AK013373 mRNA. Translation: BAB28815.1.
AK015557 mRNA. Translation: BAB29892.1.
AK045037 mRNA. Translation: BAC32192.1.
AK165072 mRNA. Translation: BAE38024.1.
AK166362 mRNA. Translation: BAE38732.1.
BC049557 mRNA. Translation: AAH49557.1.
CCDSiCCDS18387.1. [Q9CQ82-1]
RefSeqiNP_080624.1. NM_026348.3. [Q9CQ82-1]
UniGeneiMm.257094.

Genome annotation databases

EnsembliENSMUST00000146258; ENSMUSP00000117153; ENSMUSG00000028549. [Q9CQ82-1]
GeneIDi67733.
KEGGimmu:67733.
UCSCiuc008tux.1. mouse. [Q9CQ82-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005779 mRNA. Translation: BAB24235.1.
AK013373 mRNA. Translation: BAB28815.1.
AK015557 mRNA. Translation: BAB29892.1.
AK045037 mRNA. Translation: BAC32192.1.
AK165072 mRNA. Translation: BAE38024.1.
AK166362 mRNA. Translation: BAE38732.1.
BC049557 mRNA. Translation: AAH49557.1.
CCDSiCCDS18387.1. [Q9CQ82-1]
RefSeqiNP_080624.1. NM_026348.3. [Q9CQ82-1]
UniGeneiMm.257094.

3D structure databases

ProteinModelPortaliQ9CQ82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117153.

PTM databases

iPTMnetiQ9CQ82.
PhosphoSitePlusiQ9CQ82.

Proteomic databases

PaxDbiQ9CQ82.
PeptideAtlasiQ9CQ82.
PRIDEiQ9CQ82.

Protocols and materials databases

DNASUi67733.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000146258; ENSMUSP00000117153; ENSMUSG00000028549. [Q9CQ82-1]
GeneIDi67733.
KEGGimmu:67733.
UCSCiuc008tux.1. mouse. [Q9CQ82-1]

Organism-specific databases

CTDi23421.
MGIiMGI:1914983. Itgb3bp.

Phylogenomic databases

eggNOGiENOG410J05S. Eukaryota.
ENOG410Z43R. LUCA.
GeneTreeiENSGT00390000004336.
HOGENOMiHOG000049271.
HOVERGENiHBG080553.
InParanoidiQ9CQ82.
KOiK11510.
OMAiCHLDSYE.
OrthoDBiEOG091G180Q.
PhylomeDBiQ9CQ82.
TreeFamiTF336291.

Enzyme and pathway databases

ReactomeiR-MMU-205043. NRIF signals cell death from the nucleus.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-MMU-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ9CQ82.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028549.
CleanExiMM_ITGB3BP.
GenevisibleiQ9CQ82. MM.

Family and domain databases

InterProiIPR009601. CENP-R.
[Graphical view]
PANTHERiPTHR15581. PTHR15581. 1 hit.
PfamiPF06729. CENP-R. 1 hit.
[Graphical view]
PIRSFiPIRSF011860. NRIF3_coact_rcpt. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCENPR_MOUSE
AccessioniPrimary (citable) accession number: Q9CQ82
Secondary accession number(s): Q3TLR0, Q3TNR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.