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Protein

Vacuolar protein-sorting-associated protein 25

Gene

Vps25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 25
Alternative name(s):
ESCRT-II complex subunit VPS25
Gene namesi
Name:Vps25
Synonyms:D11Wsu68e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:106354. Vps25.

Subcellular locationi

  • Cytoplasm By similarity
  • Endosome membrane By similarity
  • Nucleus By similarity

  • Note: Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152171 – 176Vacuolar protein-sorting-associated protein 25Add BLAST176

Proteomic databases

EPDiQ9CQ80.
PaxDbiQ9CQ80.
PeptideAtlasiQ9CQ80.
PRIDEiQ9CQ80.

PTM databases

PhosphoSitePlusiQ9CQ80.

Expressioni

Gene expression databases

BgeeiENSMUSG00000078656.
CleanExiMM_VPS25.
ExpressionAtlasiQ9CQ80. baseline and differential.
GenevisibleiQ9CQ80. MM.

Interactioni

Subunit structurei

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and 2 copies of VPS25. Interacts with CFTR; the interaction requires misfolded CFTR. Interacts (via C-terminal half) with the ESCRT-III subunit CHMP6 (via N-terminal half) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205754. 4 interactors.
IntActiQ9CQ80. 5 interactors.
MINTiMINT-1848285.
STRINGi10090.ENSMUSP00000042088.

Structurei

3D structure databases

ProteinModelPortaliQ9CQ80.
SMRiQ9CQ80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS25 family.Curated

Phylogenomic databases

eggNOGiKOG4068. Eukaryota.
ENOG4111F6Y. LUCA.
GeneTreeiENSGT00390000014892.
HOGENOMiHOG000191978.
HOVERGENiHBG080015.
InParanoidiQ9CQ80.
KOiK12189.
PhylomeDBiQ9CQ80.
TreeFamiTF317731.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.570. 1 hit.
InterProiIPR008570. ESCRT-II_cplx_vps25-sub.
IPR014041. ESCRT-II_cplx_Vps25-sub_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13149. PTHR13149. 1 hit.
PfamiPF05871. ESCRT-II. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CQ80-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT
60 70 80 90 100
VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKNKSSFLIM
110 120 130 140 150
WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TSGEDTEDEE FHGLDEATLL
160 170
RALQALQQEH KAEIITVSDG RGVKFF
Length:176
Mass (Da):20,748
Last modified:June 1, 2001 - v1
Checksum:iB85B3A4617BA40A3
GO
Isoform 2 (identifier: Q9CQ80-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-176: EFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF → ASSRFVGVS

Note: No experimental confirmation available.
Show »
Length:148
Mass (Da):17,488
Checksum:i38EF501CE2B3545F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015341140 – 176EFHGL…GVKFF → ASSRFVGVS in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002500 mRNA. Translation: BAB22148.1.
AK003715 mRNA. Translation: BAB22955.1.
AK003877 mRNA. Translation: BAB23054.1.
AK145811 mRNA. Translation: BAE26665.1.
AK150822 mRNA. Translation: BAE29884.1.
AK166008 mRNA. Translation: BAE38516.1.
AL590969 Genomic DNA. Translation: CAM19564.1.
AL590969 Genomic DNA. Translation: CAM19566.1.
BC029181 mRNA. Translation: AAH29181.1.
CCDSiCCDS25458.1. [Q9CQ80-1]
CCDS70312.1. [Q9CQ80-2]
RefSeqiNP_001271340.1. NM_001284411.1.
NP_001271343.1. NM_001284414.1. [Q9CQ80-2]
NP_081052.2. NM_026776.4. [Q9CQ80-1]
UniGeneiMm.301020.

Genome annotation databases

EnsembliENSMUST00000007533; ENSMUSP00000007533; ENSMUSG00000078656. [Q9CQ80-2]
ENSMUST00000042477; ENSMUSP00000042088; ENSMUSG00000078656. [Q9CQ80-1]
GeneIDi28084.
KEGGimmu:28084.
UCSCiuc007loa.2. mouse. [Q9CQ80-1]
uc007lob.2. mouse. [Q9CQ80-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002500 mRNA. Translation: BAB22148.1.
AK003715 mRNA. Translation: BAB22955.1.
AK003877 mRNA. Translation: BAB23054.1.
AK145811 mRNA. Translation: BAE26665.1.
AK150822 mRNA. Translation: BAE29884.1.
AK166008 mRNA. Translation: BAE38516.1.
AL590969 Genomic DNA. Translation: CAM19564.1.
AL590969 Genomic DNA. Translation: CAM19566.1.
BC029181 mRNA. Translation: AAH29181.1.
CCDSiCCDS25458.1. [Q9CQ80-1]
CCDS70312.1. [Q9CQ80-2]
RefSeqiNP_001271340.1. NM_001284411.1.
NP_001271343.1. NM_001284414.1. [Q9CQ80-2]
NP_081052.2. NM_026776.4. [Q9CQ80-1]
UniGeneiMm.301020.

3D structure databases

ProteinModelPortaliQ9CQ80.
SMRiQ9CQ80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205754. 4 interactors.
IntActiQ9CQ80. 5 interactors.
MINTiMINT-1848285.
STRINGi10090.ENSMUSP00000042088.

PTM databases

PhosphoSitePlusiQ9CQ80.

Proteomic databases

EPDiQ9CQ80.
PaxDbiQ9CQ80.
PeptideAtlasiQ9CQ80.
PRIDEiQ9CQ80.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007533; ENSMUSP00000007533; ENSMUSG00000078656. [Q9CQ80-2]
ENSMUST00000042477; ENSMUSP00000042088; ENSMUSG00000078656. [Q9CQ80-1]
GeneIDi28084.
KEGGimmu:28084.
UCSCiuc007loa.2. mouse. [Q9CQ80-1]
uc007lob.2. mouse. [Q9CQ80-2]

Organism-specific databases

CTDi84313.
MGIiMGI:106354. Vps25.

Phylogenomic databases

eggNOGiKOG4068. Eukaryota.
ENOG4111F6Y. LUCA.
GeneTreeiENSGT00390000014892.
HOGENOMiHOG000191978.
HOVERGENiHBG080015.
InParanoidiQ9CQ80.
KOiK12189.
PhylomeDBiQ9CQ80.
TreeFamiTF317731.

Enzyme and pathway databases

ReactomeiR-MMU-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

ChiTaRSiVps25. mouse.
PROiQ9CQ80.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078656.
CleanExiMM_VPS25.
ExpressionAtlasiQ9CQ80. baseline and differential.
GenevisibleiQ9CQ80. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.570. 1 hit.
InterProiIPR008570. ESCRT-II_cplx_vps25-sub.
IPR014041. ESCRT-II_cplx_Vps25-sub_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13149. PTHR13149. 1 hit.
PfamiPF05871. ESCRT-II. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiVPS25_MOUSE
AccessioniPrimary (citable) accession number: Q9CQ80
Secondary accession number(s): A2A4J9
, A2A4K1, Q3UBT0, Q9D167
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.