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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Gene

Ndufa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: MGI
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
Alternative name(s):
Complex I-B8
Short name:
CI-B8
NADH-ubiquinone oxidoreductase B8 subunit
Gene namesi
Name:Ndufa2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1343103. Ndufa2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 9998NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2PRO_0000118790Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Disulfide bondi24 ↔ 58Redox-activeBy similarity
Modified residuei64 – 641N6-acetyllysine; alternateCombined sources
Modified residuei64 – 641N6-succinyllysine; alternateCombined sources
Modified residuei75 – 751N6-acetyllysineCombined sources

Post-translational modificationi

Acetylation of Lys-64 and Lys-75 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

EPDiQ9CQ75.
MaxQBiQ9CQ75.
PaxDbiQ9CQ75.
PeptideAtlasiQ9CQ75.
PRIDEiQ9CQ75.

PTM databases

iPTMnetiQ9CQ75.
PhosphoSiteiQ9CQ75.

Expressioni

Gene expression databases

BgeeiQ9CQ75.
GenevisibleiQ9CQ75. MM.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.By similarity

Protein-protein interaction databases

BioGridi201716. 1 interaction.
IntActiQ9CQ75. 4 interactions.
MINTiMINT-1860636.
STRINGi10090.ENSMUSP00000014438.

Structurei

3D structure databases

ProteinModelPortaliQ9CQ75.
SMRiQ9CQ75. Positions 15-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiKOG3446. Eukaryota.
ENOG41124JV. LUCA.
GeneTreeiENSGT00390000006178.
HOGENOMiHOG000201916.
HOVERGENiHBG082012.
InParanoidiQ9CQ75.
KOiK03946.
OMAiSAGTRQF.
OrthoDBiEOG779P1D.
PhylomeDBiQ9CQ75.
TreeFamiTF300229.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR016464. NADH_Ub_cplx-1_asu_su-2.
IPR007741. Ribosome/NADH_DH.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR12878. PTHR12878. 1 hit.
PfamiPF05047. L51_S25_CI-B8. 1 hit.
[Graphical view]
PIRSFiPIRSF005822. NDUA2. 1 hit.
SMARTiSM00916. L51_S25_CI-B8. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQ75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAASRAV GAKLGLREIR VHLCQRSPGS QGVRDFIVQR YVELKKAHPN
60 70 80 90
LPILIRECSE VQPKLWARYA FGQEKTVSLN NLSADEVTRA MQNVLSGKA
Length:99
Mass (Da):10,916
Last modified:January 23, 2007 - v3
Checksum:iBB06EFCADD6E30EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121A → T in AAD30475 (PubMed:10318868).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124786 mRNA. Translation: AAD30475.1.
AK003608 mRNA. Translation: BAB22887.1.
AK018732 mRNA. Translation: BAB31375.1.
BC006815 mRNA. Translation: AAH06815.1.
CCDSiCCDS29161.1.
RefSeqiNP_035015.2. NM_010885.5.
UniGeneiMm.29867.

Genome annotation databases

EnsembliENSMUST00000014438; ENSMUSP00000014438; ENSMUSG00000014294.
GeneIDi17991.
KEGGimmu:17991.
UCSCiuc008eoj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124786 mRNA. Translation: AAD30475.1.
AK003608 mRNA. Translation: BAB22887.1.
AK018732 mRNA. Translation: BAB31375.1.
BC006815 mRNA. Translation: AAH06815.1.
CCDSiCCDS29161.1.
RefSeqiNP_035015.2. NM_010885.5.
UniGeneiMm.29867.

3D structure databases

ProteinModelPortaliQ9CQ75.
SMRiQ9CQ75. Positions 15-99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201716. 1 interaction.
IntActiQ9CQ75. 4 interactions.
MINTiMINT-1860636.
STRINGi10090.ENSMUSP00000014438.

PTM databases

iPTMnetiQ9CQ75.
PhosphoSiteiQ9CQ75.

Proteomic databases

EPDiQ9CQ75.
MaxQBiQ9CQ75.
PaxDbiQ9CQ75.
PeptideAtlasiQ9CQ75.
PRIDEiQ9CQ75.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014438; ENSMUSP00000014438; ENSMUSG00000014294.
GeneIDi17991.
KEGGimmu:17991.
UCSCiuc008eoj.2. mouse.

Organism-specific databases

CTDi4695.
MGIiMGI:1343103. Ndufa2.

Phylogenomic databases

eggNOGiKOG3446. Eukaryota.
ENOG41124JV. LUCA.
GeneTreeiENSGT00390000006178.
HOGENOMiHOG000201916.
HOVERGENiHBG082012.
InParanoidiQ9CQ75.
KOiK03946.
OMAiSAGTRQF.
OrthoDBiEOG779P1D.
PhylomeDBiQ9CQ75.
TreeFamiTF300229.

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.
R-MMU-6799198. Complex I biogenesis.

Miscellaneous databases

PROiQ9CQ75.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CQ75.
GenevisibleiQ9CQ75. MM.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR016464. NADH_Ub_cplx-1_asu_su-2.
IPR007741. Ribosome/NADH_DH.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR12878. PTHR12878. 1 hit.
PfamiPF05047. L51_S25_CI-B8. 1 hit.
[Graphical view]
PIRSFiPIRSF005822. NDUA2. 1 hit.
SMARTiSM00916. L51_S25_CI-B8. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Up-regulation of nuclear and mitochondrial genes in the skeletal muscle of mice lacking the heart/muscle isoform of the adenine nucleotide translocator."
    Murdock D.G., Boone B.E., Esposito L.A., Wallace D.C.
    J. Biol. Chem. 274:14429-14433(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Skeletal muscle.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo and Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 47-56 AND 76-98, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-64 AND LYS-75, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiNDUA2_MOUSE
AccessioniPrimary (citable) accession number: Q9CQ75
Secondary accession number(s): Q9WUB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.