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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

Mtap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (Mtap)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18PhosphateUniRule annotation1
Sitei178Important for substrate specificityUniRule annotation1
Binding sitei196Substrate; via amide nitrogenUniRule annotation1
Binding sitei197PhosphateUniRule annotation1
Sitei233Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • L-methionine salvage from methylthioadenosine Source: UniProtKB-UniPathway
  • methylation Source: MGI
  • purine ribonucleoside salvage Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

BRENDAi2.4.2.28 3474
ReactomeiR-MMU-1237112 Methionine salvage pathway
UniPathwayiUPA00904; UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
Name:Mtap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914152 Mtap

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2663

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001845461 – 283S-methyl-5'-thioadenosine phosphorylaseAdd BLAST283

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9CQ65
MaxQBiQ9CQ65
PaxDbiQ9CQ65
PeptideAtlasiQ9CQ65
PRIDEiQ9CQ65

2D gel databases

REPRODUCTION-2DPAGEQ9CQ65

PTM databases

iPTMnetiQ9CQ65
PhosphoSitePlusiQ9CQ65
SwissPalmiQ9CQ65

Expressioni

Gene expression databases

BgeeiENSMUSG00000062937
CleanExiMM_MTAP
GenevisibleiQ9CQ65 MM

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

BioGridi211799, 4 interactors
IntActiQ9CQ65, 2 interactors
MINTiQ9CQ65
STRINGi10090.ENSMUSP00000061092

Chemistry databases

BindingDBiQ9CQ65

Structurei

3D structure databases

ProteinModelPortaliQ9CQ65
SMRiQ9CQ65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 61Phosphate bindingUniRule annotation2
Regioni93 – 94Phosphate bindingUniRule annotation2
Regioni220 – 222Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3985 Eukaryota
COG0005 LUCA
GeneTreeiENSGT00550000074874
HOGENOMiHOG000228986
HOVERGENiHBG002487
InParanoidiQ9CQ65
KOiK00772
OMAiNLYRSWG
OrthoDBiEOG091G0CQI
PhylomeDBiQ9CQ65
TreeFamiTF312883

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CQ65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGSACTAV KIGIIGGTGL DDPEILEGRT EKYVDTPFGK PSDALILGKI
60 70 80 90 100
KNVDCVLLAR HGRQHTIMPS KVNYQANIWA LKEEGCTHVI VTTACGSLRE
110 120 130 140 150
EIQPGDMVII DQFIDRTSLR PQTFYDGSHC SARGVCHIPM AEPFCPKTRE
160 170 180 190 200
VLIETAKKLG LRCHSKGTIV TIEGPRFSSR AESLIFRTWG ADVVNMTTVP
210 220 230 240 250
EVVLAKEAGI CYASIAMATD YDCWKEHEEA VSVDGVLKTM KENANKAKSL
260 270 280
LLTTIPQIGS MEWSETLRNL KNMAQFSVLP PRH
Length:283
Mass (Da):31,062
Last modified:June 1, 2001 - v1
Checksum:iBF64441F41AE81EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056100 mRNA Translation: BAB32865.1
AK005064 mRNA Translation: BAB23788.1
AK011421 mRNA Translation: BAB27609.1
AK167319 mRNA Translation: BAE39421.1
BC003858 mRNA Translation: AAH03858.1
CCDSiCCDS18349.1
RefSeqiNP_077753.1, NM_024433.2
UniGeneiMm.28500

Genome annotation databases

EnsembliENSMUST00000058030; ENSMUSP00000061092; ENSMUSG00000062937
GeneIDi66902
KEGGimmu:66902
UCSCiuc008tof.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMTAP_MOUSE
AccessioniPrimary (citable) accession number: Q9CQ65
Secondary accession number(s): Q3TJS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 24, 2002
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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