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Protein

Nuclear cap-binding protein subunit 2

Gene

Ncbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20mRNA capBy similarity1
Binding sitei43mRNA capBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, RNA-mediated gene silencing, Translation regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-111367 SLBP independent Processing of Histone Pre-mRNAs
R-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-191859 snRNP Assembly
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6803529 FGFR2 alternative splicing
R-MMU-6807505 RNA polymerase II transcribes snRNA genes
R-MMU-72086 mRNA Capping
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway
R-MMU-72187 mRNA 3'-end processing
R-MMU-72203 Processing of Capped Intron-Containing Pre-mRNA
R-MMU-77588 SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs
R-MMU-77595 Processing of Intronless Pre-mRNAs
R-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear cap-binding protein subunit 2
Alternative name(s):
20 kDa nuclear cap-binding protein
NCBP 20 kDa subunit
Short name:
CBP20
Gene namesi
Name:Ncbp2
Synonyms:Cbp20
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1915342 Ncbp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000815002 – 156Nuclear cap-binding protein subunit 2Add BLAST155

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei13PhosphoserineCombined sources1
Modified residuei18PhosphoserineBy similarity1
Modified residuei146Omega-N-methylarginineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9CQ49
MaxQBiQ9CQ49
PaxDbiQ9CQ49
PeptideAtlasiQ9CQ49
PRIDEiQ9CQ49

PTM databases

iPTMnetiQ9CQ49
PhosphoSitePlusiQ9CQ49

Expressioni

Gene expression databases

BgeeiENSMUSG00000022774
CleanExiMM_NCBP2
ExpressionAtlasiQ9CQ49 baseline and differential
GenevisibleiQ9CQ49 MM

Interactioni

Subunit structurei

Component of the nuclear cap-binding complex (CBC), a heterodimer composed of NCBP1/CBP80 and NCBP2/CBP20 that interacts with m7GpppG-capped RNA (By similarity). Found in a U snRNA export complex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA (PubMed:10786834). Interacts with PHAX/RNUXA, EIF4G1, HNRNPF, HNRNPH1 and ALYREF/THOC4/ALY (By similarity). Interacts with SRRT/ARS2 and KPNA3 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi212651, 25 interactors
IntActiQ9CQ49, 26 interactors
MINTiQ9CQ49
STRINGi10090.ENSMUSP00000023460

Structurei

3D structure databases

ProteinModelPortaliQ9CQ49
SMRiQ9CQ49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 118RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni112 – 116mRNA cap-bindingBy similarity5
Regioni123 – 127mRNA cap-bindingBy similarity5
Regioni133 – 134mRNA cap-bindingBy similarity2

Sequence similaritiesi

Belongs to the RRM NCBP2 family.Curated

Phylogenomic databases

eggNOGiKOG0121 Eukaryota
ENOG4111FJQ LUCA
GeneTreeiENSGT00390000003197
HOGENOMiHOG000217589
HOVERGENiHBG052581
InParanoidiQ9CQ49
KOiK12883
OMAiCMKYIGG
OrthoDBiEOG091G0RKX
PhylomeDBiQ9CQ49
TreeFamiTF313897

Family and domain databases

CDDicd12240 RRM_NCBP2, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR027157 NCBP2
IPR034148 NCBP2_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR18847 PTHR18847, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQ49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGLLKALR SDSYVELSEY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY
60 70 80 90 100
TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY
110 120 130 140 150
INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRE DYDAGRGGYG

KLAQKQ
Length:156
Mass (Da):18,017
Last modified:June 1, 2001 - v1
Checksum:iC6DF61E0C6A2CC2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008980 mRNA Translation: BAB26004.1
AK012659 mRNA Translation: BAB28389.1
AK077841 mRNA Translation: BAC37030.1
AK147027 mRNA Translation: BAE27620.1
CH466521 Genomic DNA Translation: EDK97760.1
BC118926 mRNA Translation: AAI18927.1
CCDSiCCDS28110.1
RefSeqiNP_080830.1, NM_026554.4
UniGeneiMm.290027
Mm.456900

Genome annotation databases

EnsembliENSMUST00000023460; ENSMUSP00000023460; ENSMUSG00000022774
GeneIDi68092
KEGGimmu:68092
UCSCiuc007yxz.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNCBP2_MOUSE
AccessioniPrimary (citable) accession number: Q9CQ49
Secondary accession number(s): Q0VF93, Q3UI86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health