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Q9CQ39

- MED21_MOUSE

UniProt

Q9CQ39 - MED21_MOUSE

Protein

Mediator of RNA polymerase II transcription subunit 21

Gene

Med21

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. transcription coactivator activity Source: Ensembl

    GO - Biological processi

    1. blastocyst development Source: MGI
    2. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    3. stem cell maintenance Source: MGI
    4. transcription from RNA polymerase II promoter Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 21
    Alternative name(s):
    Mediator complex subunit 21
    RNA polymerase II holoenzyme component SRB7
    Short name:
    RNAPII complex component SRB7
    Gene namesi
    Name:Med21
    Synonyms:Srb7, Surb7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1347064. Med21.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: MGI
    2. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 144144Mediator of RNA polymerase II transcription subunit 21PRO_0000305947Add
    BLAST

    Proteomic databases

    PaxDbiQ9CQ39.
    PRIDEiQ9CQ39.

    PTM databases

    PhosphoSiteiQ9CQ39.

    Expressioni

    Tissue specificityi

    Expressed in brain, heart, kidney, liver, lung, skeletal muscles, spleen and testis.1 Publication

    Gene expression databases

    ArrayExpressiQ9CQ39.
    BgeeiQ9CQ39.
    CleanExiMM_MED21.
    GenevestigatoriQ9CQ39.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a ligand-dependent fashion By similarity. Interacts with PPARG.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi223831. 9 interactions.
    DIPiDIP-59522N.
    IntActiQ9CQ39. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CQ39.
    SMRiQ9CQ39. Positions 3-129.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 21 family.Curated

    Phylogenomic databases

    eggNOGiNOG247021.
    GeneTreeiENSGT00390000014557.
    HOGENOMiHOG000006767.
    HOVERGENiHBG009229.
    InParanoidiQ9CQ39.
    KOiK15152.
    OMAiQAEHFCN.
    OrthoDBiEOG780RPZ.
    PhylomeDBiQ9CQ39.

    Family and domain databases

    InterProiIPR021384. Mediator_Med21.
    [Graphical view]
    PfamiPF11221. Med21. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CQ39-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFSNIQT AINKDQPANP    50
    TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA 100
    ATCLEDVVYR GDMLLEKIQS ALADIAQSQL KTRSVTHSHS LPDS 144
    Length:144
    Mass (Da):15,588
    Last modified:June 1, 2001 - v1
    Checksum:iCCFEA1DF0B6B53AC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti137 – 1371H → R in AAH12286. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK009742 mRNA. Translation: BAB26472.1.
    AK012217 mRNA. Translation: BAB28104.1.
    AK014428 mRNA. Translation: BAB29343.1.
    AK019417 mRNA. Translation: BAB31711.1.
    BC012286 mRNA. Translation: AAH12286.1.
    CCDSiCCDS20700.1.
    RefSeqiNP_079591.1. NM_025315.3.
    UniGeneiMm.26212.

    Genome annotation databases

    EnsembliENSMUST00000032429; ENSMUSP00000032429; ENSMUSG00000030291.
    GeneIDi108098.
    KEGGimmu:108098.
    UCSCiuc009esg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK009742 mRNA. Translation: BAB26472.1 .
    AK012217 mRNA. Translation: BAB28104.1 .
    AK014428 mRNA. Translation: BAB29343.1 .
    AK019417 mRNA. Translation: BAB31711.1 .
    BC012286 mRNA. Translation: AAH12286.1 .
    CCDSi CCDS20700.1.
    RefSeqi NP_079591.1. NM_025315.3.
    UniGenei Mm.26212.

    3D structure databases

    ProteinModelPortali Q9CQ39.
    SMRi Q9CQ39. Positions 3-129.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 223831. 9 interactions.
    DIPi DIP-59522N.
    IntActi Q9CQ39. 3 interactions.

    PTM databases

    PhosphoSitei Q9CQ39.

    Proteomic databases

    PaxDbi Q9CQ39.
    PRIDEi Q9CQ39.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032429 ; ENSMUSP00000032429 ; ENSMUSG00000030291 .
    GeneIDi 108098.
    KEGGi mmu:108098.
    UCSCi uc009esg.1. mouse.

    Organism-specific databases

    CTDi 9412.
    MGIi MGI:1347064. Med21.

    Phylogenomic databases

    eggNOGi NOG247021.
    GeneTreei ENSGT00390000014557.
    HOGENOMi HOG000006767.
    HOVERGENi HBG009229.
    InParanoidi Q9CQ39.
    KOi K15152.
    OMAi QAEHFCN.
    OrthoDBi EOG780RPZ.
    PhylomeDBi Q9CQ39.

    Enzyme and pathway databases

    Reactomei REACT_198602. PPARA activates gene expression.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    NextBioi 360058.
    PROi Q9CQ39.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9CQ39.
    Bgeei Q9CQ39.
    CleanExi MM_MED21.
    Genevestigatori Q9CQ39.

    Family and domain databases

    InterProi IPR021384. Mediator_Med21.
    [Graphical view ]
    Pfami PF11221. Med21. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo, Head, Placenta and Tongue.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    3. "Ubiquitous expression and embryonic requirement for RNA polymerase II coactivator subunit Srb7 in mice."
      Tudor M., Murray P.J., Onufryk C., Jaenisch R., Young R.A.
      Genes Dev. 13:2365-2368(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis."
      Ge K., Guermah M., Yuan C.-X., Ito M., Wallberg A.E., Spiegelman B.M., Roeder R.G.
      Nature 417:563-567(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PPARG.

    Entry informationi

    Entry nameiMED21_MOUSE
    AccessioniPrimary (citable) accession number: Q9CQ39
    Secondary accession number(s): Q921I5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3