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Q9CQ39

- MED21_MOUSE

UniProt

Q9CQ39 - MED21_MOUSE

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Protein

Mediator of RNA polymerase II transcription subunit 21

Gene

Med21

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

  1. transcription coactivator activity Source: Ensembl

GO - Biological processi

  1. blastocyst development Source: MGI
  2. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  3. stem cell maintenance Source: MGI
  4. transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_198602. PPARA activates gene expression.
REACT_252217. Transcriptional regulation of white adipocyte differentiation.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 21
Alternative name(s):
Mediator complex subunit 21
RNA polymerase II holoenzyme component SRB7
Short name:
RNAPII complex component SRB7
Gene namesi
Name:Med21
Synonyms:Srb7, Surb7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1347064. Med21.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: MGI
  2. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Mediator of RNA polymerase II transcription subunit 21PRO_0000305947Add
BLAST

Proteomic databases

MaxQBiQ9CQ39.
PaxDbiQ9CQ39.
PRIDEiQ9CQ39.

PTM databases

PhosphoSiteiQ9CQ39.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, skeletal muscles, spleen and testis.1 Publication

Gene expression databases

BgeeiQ9CQ39.
CleanExiMM_MED21.
ExpressionAtlasiQ9CQ39. baseline and differential.
GenevestigatoriQ9CQ39.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a ligand-dependent fashion (By similarity). Interacts with PPARG.By similarity1 Publication

Protein-protein interaction databases

BioGridi223831. 9 interactions.
DIPiDIP-59522N.
IntActiQ9CQ39. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9CQ39.
SMRiQ9CQ39. Positions 3-129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 21 family.Curated

Phylogenomic databases

eggNOGiNOG247021.
GeneTreeiENSGT00390000014557.
HOGENOMiHOG000006767.
HOVERGENiHBG009229.
InParanoidiQ9CQ39.
KOiK15152.
OMAiQAEHFCN.
OrthoDBiEOG780RPZ.
PhylomeDBiQ9CQ39.

Family and domain databases

InterProiIPR021384. Mediator_Med21.
[Graphical view]
PfamiPF11221. Med21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CQ39-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFSNIQT AINKDQPANP
60 70 80 90 100
TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA
110 120 130 140
ATCLEDVVYR GDMLLEKIQS ALADIAQSQL KTRSVTHSHS LPDS
Length:144
Mass (Da):15,588
Last modified:June 1, 2001 - v1
Checksum:iCCFEA1DF0B6B53AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti137 – 1371H → R in AAH12286. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009742 mRNA. Translation: BAB26472.1.
AK012217 mRNA. Translation: BAB28104.1.
AK014428 mRNA. Translation: BAB29343.1.
AK019417 mRNA. Translation: BAB31711.1.
BC012286 mRNA. Translation: AAH12286.1.
CCDSiCCDS20700.1.
RefSeqiNP_079591.1. NM_025315.3.
UniGeneiMm.26212.

Genome annotation databases

EnsembliENSMUST00000032429; ENSMUSP00000032429; ENSMUSG00000030291.
GeneIDi108098.
KEGGimmu:108098.
UCSCiuc009esg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009742 mRNA. Translation: BAB26472.1 .
AK012217 mRNA. Translation: BAB28104.1 .
AK014428 mRNA. Translation: BAB29343.1 .
AK019417 mRNA. Translation: BAB31711.1 .
BC012286 mRNA. Translation: AAH12286.1 .
CCDSi CCDS20700.1.
RefSeqi NP_079591.1. NM_025315.3.
UniGenei Mm.26212.

3D structure databases

ProteinModelPortali Q9CQ39.
SMRi Q9CQ39. Positions 3-129.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 223831. 9 interactions.
DIPi DIP-59522N.
IntActi Q9CQ39. 3 interactions.

PTM databases

PhosphoSitei Q9CQ39.

Proteomic databases

MaxQBi Q9CQ39.
PaxDbi Q9CQ39.
PRIDEi Q9CQ39.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032429 ; ENSMUSP00000032429 ; ENSMUSG00000030291 .
GeneIDi 108098.
KEGGi mmu:108098.
UCSCi uc009esg.1. mouse.

Organism-specific databases

CTDi 9412.
MGIi MGI:1347064. Med21.

Phylogenomic databases

eggNOGi NOG247021.
GeneTreei ENSGT00390000014557.
HOGENOMi HOG000006767.
HOVERGENi HBG009229.
InParanoidi Q9CQ39.
KOi K15152.
OMAi QAEHFCN.
OrthoDBi EOG780RPZ.
PhylomeDBi Q9CQ39.

Enzyme and pathway databases

Reactomei REACT_198602. PPARA activates gene expression.
REACT_252217. Transcriptional regulation of white adipocyte differentiation.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

NextBioi 360058.
PROi Q9CQ39.
SOURCEi Search...

Gene expression databases

Bgeei Q9CQ39.
CleanExi MM_MED21.
ExpressionAtlasi Q9CQ39. baseline and differential.
Genevestigatori Q9CQ39.

Family and domain databases

InterProi IPR021384. Mediator_Med21.
[Graphical view ]
Pfami PF11221. Med21. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Head, Placenta and Tongue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  3. "Ubiquitous expression and embryonic requirement for RNA polymerase II coactivator subunit Srb7 in mice."
    Tudor M., Murray P.J., Onufryk C., Jaenisch R., Young R.A.
    Genes Dev. 13:2365-2368(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis."
    Ge K., Guermah M., Yuan C.-X., Ito M., Wallberg A.E., Spiegelman B.M., Roeder R.G.
    Nature 417:563-567(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPARG.

Entry informationi

Entry nameiMED21_MOUSE
AccessioniPrimary (citable) accession number: Q9CQ39
Secondary accession number(s): Q921I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3