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Q9CQ39 (MED21_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 21
Alternative name(s):
Mediator complex subunit 21
RNA polymerase II holoenzyme component SRB7
Short name=RNAPII complex component SRB7
Gene names
Name:Med21
Synonyms:Srb7, Surb7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length144 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.

Subunit structure

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a ligand-dependent fashion By similarity. Interacts with PPARG. Ref.4

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in brain, heart, kidney, liver, lung, skeletal muscles, spleen and testis. Ref.3

Sequence similarities

Belongs to the Mediator complex subunit 21 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 144144Mediator of RNA polymerase II transcription subunit 21
PRO_0000305947

Experimental info

Sequence conflict1371H → R in AAH12286. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9CQ39 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: CCFEA1DF0B6B53AC

FASTA14415,588
        10         20         30         40         50         60 
MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFSNIQT AINKDQPANP TEEYAQLFAA 

        70         80         90        100        110        120 
LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA ATCLEDVVYR GDMLLEKIQS 

       130        140 
ALADIAQSQL KTRSVTHSHS LPDS 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo, Head, Placenta and Tongue.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[3]"Ubiquitous expression and embryonic requirement for RNA polymerase II coactivator subunit Srb7 in mice."
Tudor M., Murray P.J., Onufryk C., Jaenisch R., Young R.A.
Genes Dev. 13:2365-2368(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis."
Ge K., Guermah M., Yuan C.-X., Ito M., Wallberg A.E., Spiegelman B.M., Roeder R.G.
Nature 417:563-567(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPARG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK009742 mRNA. Translation: BAB26472.1.
AK012217 mRNA. Translation: BAB28104.1.
AK014428 mRNA. Translation: BAB29343.1.
AK019417 mRNA. Translation: BAB31711.1.
BC012286 mRNA. Translation: AAH12286.1.
CCDSCCDS20700.1.
RefSeqNP_079591.1. NM_025315.3.
UniGeneMm.26212.

3D structure databases

ProteinModelPortalQ9CQ39.
SMRQ9CQ39. Positions 3-129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid223831. 9 interactions.
DIPDIP-59522N.
IntActQ9CQ39. 3 interactions.

PTM databases

PhosphoSiteQ9CQ39.

Proteomic databases

PaxDbQ9CQ39.
PRIDEQ9CQ39.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032429; ENSMUSP00000032429; ENSMUSG00000030291.
GeneID108098.
KEGGmmu:108098.
UCSCuc009esg.1. mouse.

Organism-specific databases

CTD9412.
MGIMGI:1347064. Med21.

Phylogenomic databases

eggNOGNOG247021.
GeneTreeENSGT00390000014557.
HOGENOMHOG000006767.
HOVERGENHBG009229.
InParanoidQ9CQ39.
KOK15152.
OMAQAEHFCN.
OrthoDBEOG780RPZ.
PhylomeDBQ9CQ39.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.
REACT_200794. Mus musculus biological processes.

Gene expression databases

ArrayExpressQ9CQ39.
BgeeQ9CQ39.
CleanExMM_MED21.
GenevestigatorQ9CQ39.

Family and domain databases

InterProIPR021384. Mediator_Med21.
[Graphical view]
PfamPF11221. Med21. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio360058.
PROQ9CQ39.
SOURCESearch...

Entry information

Entry nameMED21_MOUSE
AccessionPrimary (citable) accession number: Q9CQ39
Secondary accession number(s): Q921I5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot