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Q9CQ10

- CHMP3_MOUSE

UniProt

Q9CQ10 - CHMP3_MOUSE

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Protein

Charged multivesicular body protein 3

Gene
Chmp3, Vps24
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei48 – 481Important for autoinhibitory function By similarity
Binding sitei218 – 2181STAMBP By similarity

GO - Molecular functioni

  1. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. endosome to lysosome transport Source: Ensembl
  4. protein transport Source: UniProtKB-KW
  5. regulation of endosome size Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_198518. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 3
Alternative name(s):
Chromatin-modifying protein 3
Vacuolar protein sorting-associated protein 24
Gene namesi
Name:Chmp3
Synonyms:Vps24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1913950. Chmp3.

Subcellular locationi

Cytoplasmcytosol By similarity. Membrane; Lipid-anchor By similarity. Endosome By similarity. Late endosome membrane By similarity
Note: Localizes to the midbody of dividing cells By similarity.

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. early endosome Source: Ensembl
  3. late endosome membrane Source: UniProtKB-SubCell
  4. midbody Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed Reviewed prediction
Chaini2 – 224223Charged multivesicular body protein 3PRO_0000211481Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine Reviewed prediction
Cross-linki179 – 179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei200 – 2001Phosphoserine1 Publication

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9CQ10.
PaxDbiQ9CQ10.
PRIDEiQ9CQ10.

PTM databases

PhosphoSiteiQ9CQ10.

Expressioni

Tissue specificityi

Expressed in lung, testis, heart, spleen, skeletal muscle, kidney, liver and brain.1 Publication

Gene expression databases

BgeeiQ9CQ10.
CleanExiMM_VPS24.
GenevestigatoriQ9CQ10.

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Forms a metastable monomer in solution; its core structure (without part of the putative autoinhibitory C-terminal acidic region) oligomerizes into a flat lattice via two different dimerization interfaces. In vitro, heteromerizes with CHMP2A (but not CHMP4) to form helical tubular structures that expose membrane-interacting sites on the outside whereas VPS4B can associate on the inside of the tubule. May interact with IGFBP7; the relevance of such interaction however remains unclear. Interacts with CHMP2A. Interacts with CHMP4A; the interaction requires the release of CHMP4A autoinhibition. Interacts with VPS4A. Interacts with STAMBP; the interaction appears to relieve the autoinhibition of CHMP3 By similarity. Interacts with VTA1 By similarity.

Protein-protein interaction databases

BioGridi211654. 3 interactions.
IntActiQ9CQ10. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9CQ10.
SMRiQ9CQ10. Positions 5-181.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 113112Intramolecular interaction with C-terminus By similarityAdd
BLAST
Regioni59 – 646Important for autoinhibitory function By similarity
Regioni151 – 22474Interaction with VPS4A By similarityAdd
BLAST
Regioni151 – 22272Intramolecular interaction with N-terminus By similarityAdd
BLAST
Regioni168 – 1692Important for autoinhibitory function By similarity
Regioni196 – 22429Interaction with STAMBP By similarityAdd
BLAST
Regioni205 – 2095Interaction with STAMBP By similarity
Regioni223 – 2242Interaction with STAMBP By similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili22 – 5433 Reviewed predictionAdd
BLAST
Coiled coili149 – 22476 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi201 – 21313MIT-interacting motif By similarityAdd
BLAST

Domaini

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

Sequence similaritiesi

Belongs to the SNF7 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5491.
GeneTreeiENSGT00550000074896.
HOGENOMiHOG000177219.
HOVERGENiHBG107031.
InParanoidiQ9CQ10.
KOiK12193.
OMAiMLDETMD.
OrthoDBiEOG7D59Q2.
PhylomeDBiQ9CQ10.
TreeFamiTF105848.

Family and domain databases

InterProiIPR005024. Snf7.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQ10-1 [UniParc]FASTAAdd to Basket

« Hide

MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD    50
AAKKGQKEVC VVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV 100
AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES 150
MDDQEEMEEA AEMEIDRILF EITAGALGKA PSKVTDALPE PEPAGAMAAS 200
EEGEEEEDEE DLEAMQSRLA TLRS 224
Length:224
Mass (Da):25,219
Last modified:January 23, 2007 - v3
Checksum:iDC1A566E86D82A07
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71T → S in BAB26273. 1 Publication
Sequence conflicti161 – 1611A → V in BAB31306. 1 Publication
Sequence conflicti170 – 1701F → L in BAB31306. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK009414 mRNA. Translation: BAB26273.1.
AK014818 mRNA. Translation: BAB29566.1.
AK016677 mRNA. Translation: BAB30375.1.
AK018611 mRNA. Translation: BAB31306.1.
AK083562 mRNA. Translation: BAC38951.1.
BC049964 mRNA. Translation: AAH49964.1.
CCDSiCCDS20232.1.
RefSeqiNP_080059.2. NM_025783.3.
UniGeneiMm.181278.

Genome annotation databases

EnsembliENSMUST00000059462; ENSMUSP00000109815; ENSMUSG00000053119.
ENSMUST00000065364; ENSMUSP00000068410; ENSMUSG00000053119.
GeneIDi66700.
KEGGimmu:66700.
UCSCiuc009cgw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK009414 mRNA. Translation: BAB26273.1 .
AK014818 mRNA. Translation: BAB29566.1 .
AK016677 mRNA. Translation: BAB30375.1 .
AK018611 mRNA. Translation: BAB31306.1 .
AK083562 mRNA. Translation: BAC38951.1 .
BC049964 mRNA. Translation: AAH49964.1 .
CCDSi CCDS20232.1.
RefSeqi NP_080059.2. NM_025783.3.
UniGenei Mm.181278.

3D structure databases

ProteinModelPortali Q9CQ10.
SMRi Q9CQ10. Positions 5-181.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 211654. 3 interactions.
IntActi Q9CQ10. 2 interactions.

PTM databases

PhosphoSitei Q9CQ10.

Proteomic databases

MaxQBi Q9CQ10.
PaxDbi Q9CQ10.
PRIDEi Q9CQ10.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000059462 ; ENSMUSP00000109815 ; ENSMUSG00000053119 .
ENSMUST00000065364 ; ENSMUSP00000068410 ; ENSMUSG00000053119 .
GeneIDi 66700.
KEGGi mmu:66700.
UCSCi uc009cgw.1. mouse.

Organism-specific databases

CTDi 51652.
MGIi MGI:1913950. Chmp3.

Phylogenomic databases

eggNOGi COG5491.
GeneTreei ENSGT00550000074896.
HOGENOMi HOG000177219.
HOVERGENi HBG107031.
InParanoidi Q9CQ10.
KOi K12193.
OMAi MLDETMD.
OrthoDBi EOG7D59Q2.
PhylomeDBi Q9CQ10.
TreeFami TF105848.

Enzyme and pathway databases

Reactomei REACT_198518. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

NextBioi 322405.
PROi Q9CQ10.
SOURCEi Search...

Gene expression databases

Bgeei Q9CQ10.
CleanExi MM_VPS24.
Genevestigatori Q9CQ10.

Family and domain databases

InterProi IPR005024. Snf7.
[Graphical view ]
Pfami PF03357. Snf7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cecum, Testis and Tongue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. "Characterization of a novel alternatively spliced human transcript encoding an N-terminally truncated Vps24 protein that suppresses the effects of Bax in an ESCRT independent manner in yeast."
    Khoury C.M., Yang Z., Ismail S., Greenwood M.T.
    Gene 391:233-241(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHMP3_MOUSE
AccessioniPrimary (citable) accession number: Q9CQ10
Secondary accession number(s): Q9D2Z2, Q9D7A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi