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Protein

Cytosol aminopeptidase

Gene

Lap3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of N-terminal proline from a peptide.

Cofactori

Note: Binds 2 zinc ions per subunit. One zinc ion is tightly bound and essential for enzyme activity, while the second metal coordination site can be occupied by zinc, magnesium or manganese to give enzymes of different activities.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi282Zinc 2By similarity1
Metal bindingi287Zinc 1By similarity1
Metal bindingi287Zinc 2By similarity1
Active sitei294By similarity1
Metal bindingi305Zinc 2By similarity1
Metal bindingi364Zinc 1By similarity1
Metal bindingi366Zinc 1By similarity1
Metal bindingi366Zinc 2By similarity1
Active sitei368By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Zinc

Protein family/group databases

MEROPSiM17.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase 3
Short name:
LAP-3
Leucyl aminopeptidase
Proline aminopeptidase (EC:3.4.11.5)
Prolyl aminopeptidase
Gene namesi
Name:Lap3
Synonyms:Lapep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1914238. Lap3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001658261 – 519Cytosol aminopeptidaseAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45N6-succinyllysineCombined sources1
Modified residuei54PhosphoserineBy similarity1
Modified residuei61N6-succinyllysineCombined sources1
Modified residuei103N6-succinyllysineCombined sources1
Modified residuei180PhosphoserineCombined sources1
Modified residuei194PhosphoserineBy similarity1
Modified residuei221N6-acetyllysine; alternateCombined sources1
Modified residuei221N6-succinyllysine; alternateCombined sources1
Modified residuei238PhosphoserineCombined sources1
Modified residuei455N6-acetyllysine; alternateCombined sources1
Modified residuei455N6-succinyllysine; alternateCombined sources1
Modified residuei476N6-succinyllysineCombined sources1
Modified residuei489N6-acetyllysine; alternateCombined sources1
Modified residuei489N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9CPY7.
PeptideAtlasiQ9CPY7.
PRIDEiQ9CPY7.

2D gel databases

REPRODUCTION-2DPAGEIPI00828469.

PTM databases

iPTMnetiQ9CPY7.
PhosphoSitePlusiQ9CPY7.
SwissPalmiQ9CPY7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039682.
CleanExiMM_LAP3.
ExpressionAtlasiQ9CPY7. baseline and differential.
GenevisibleiQ9CPY7. MM.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

IntActiQ9CPY7. 5 interactors.
MINTiMINT-1869867.
STRINGi10090.ENSMUSP00000040222.

Structurei

3D structure databases

ProteinModelPortaliQ9CPY7.
SMRiQ9CPY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiKOG2597. Eukaryota.
COG0260. LUCA.
GeneTreeiENSGT00530000063255.
HOGENOMiHOG000243129.
HOVERGENiHBG003320.
InParanoidiQ9CPY7.
KOiK11142.
OMAiGIVGMKS.
OrthoDBiEOG091G0ERB.
PhylomeDBiQ9CPY7.
TreeFamiTF314954.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9CPY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYLLPLPAAA RVALRRLGVR GLWDRGLSTA DMTKGLVLGI YAKDKDDDLP
60 70 80 90 100
QFTSAGESFN KLVSGKLREM LNISGPPLKA GKTRTFYGLH QDFPSVVVVG
110 120 130 140 150
LGKRSAGVDD QENWHEGKEN IRAAVAAGCR QVQDLELPSV EVDPCGDAQA
160 170 180 190 200
AAEGAVLGLY EYDDLKQKKK VAVSAKLHGS GDLEAWEKGV LFASGQNLAR
210 220 230 240 250
HLMESPANEM TPTRFAEIIE KNLKSASSKT KVHIRPKSWI EEQEMGSFLS
260 270 280 290 300
VAKGSEEPPV FLEIHYMGSP NATEAPLVFV GKGITFDSGG ISIKASANMD
310 320 330 340 350
LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVV
360 370 380 390 400
RARNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVIINAA TLTGAMDVAL
410 420 430 440 450
GSGATGVFTN SSWLWNKLFE ASVETGDRVW RMPLFEHYTR QVIDCQLADV
460 470 480 490 500
NNLGKYRSAG ACTAAAFLRE FVTHTKWAHL DIAGVMTNKD EIPYLRKGMS
510
GRPTRTLIEF LLRFSKDSS
Length:519
Mass (Da):56,141
Last modified:January 23, 2007 - v3
Checksum:i46729A9CE4C060A5
GO
Isoform 2 (identifier: Q9CPY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: No experimental confirmation available.
Show »
Length:488
Mass (Da):52,750
Checksum:iE6800977EC37A2AC
GO

Sequence cautioni

The sequence BAB23958 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB25769 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB27726 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE39141 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE40823 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46D → G in CAJ18509 (Ref. 2) Curated1
Sequence conflicti93F → L in CAJ18445 (Ref. 2) Curated1
Sequence conflicti123A → D in BAB26987 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0226321 – 31Missing in isoform 2. CuratedAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334160 mRNA. Translation: AAK13495.1.
CT010237 mRNA. Translation: CAJ18445.1.
CT010301 mRNA. Translation: CAJ18509.1.
AK002819 mRNA. No translation available.
AK005334 mRNA. Translation: BAB23958.1. Different initiation.
AK008600 mRNA. Translation: BAB25769.1. Different initiation.
AK010502 mRNA. Translation: BAB26987.1.
AK011604 mRNA. Translation: BAB27726.1. Different initiation.
AK166958 mRNA. Translation: BAE39141.1. Different initiation.
AK169032 mRNA. Translation: BAE40823.1. Different initiation.
BC016536 mRNA. Translation: AAH16536.1.
CCDSiCCDS19274.1. [Q9CPY7-1]
RefSeqiNP_077754.3. NM_024434.6. [Q9CPY7-1]
UniGeneiMm.286830.

Genome annotation databases

EnsembliENSMUST00000046122; ENSMUSP00000040222; ENSMUSG00000039682. [Q9CPY7-1]
GeneIDi66988.
KEGGimmu:66988.
UCSCiuc008xiy.2. mouse. [Q9CPY7-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334160 mRNA. Translation: AAK13495.1.
CT010237 mRNA. Translation: CAJ18445.1.
CT010301 mRNA. Translation: CAJ18509.1.
AK002819 mRNA. No translation available.
AK005334 mRNA. Translation: BAB23958.1. Different initiation.
AK008600 mRNA. Translation: BAB25769.1. Different initiation.
AK010502 mRNA. Translation: BAB26987.1.
AK011604 mRNA. Translation: BAB27726.1. Different initiation.
AK166958 mRNA. Translation: BAE39141.1. Different initiation.
AK169032 mRNA. Translation: BAE40823.1. Different initiation.
BC016536 mRNA. Translation: AAH16536.1.
CCDSiCCDS19274.1. [Q9CPY7-1]
RefSeqiNP_077754.3. NM_024434.6. [Q9CPY7-1]
UniGeneiMm.286830.

3D structure databases

ProteinModelPortaliQ9CPY7.
SMRiQ9CPY7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CPY7. 5 interactors.
MINTiMINT-1869867.
STRINGi10090.ENSMUSP00000040222.

Protein family/group databases

MEROPSiM17.001.

PTM databases

iPTMnetiQ9CPY7.
PhosphoSitePlusiQ9CPY7.
SwissPalmiQ9CPY7.

2D gel databases

REPRODUCTION-2DPAGEIPI00828469.

Proteomic databases

PaxDbiQ9CPY7.
PeptideAtlasiQ9CPY7.
PRIDEiQ9CPY7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046122; ENSMUSP00000040222; ENSMUSG00000039682. [Q9CPY7-1]
GeneIDi66988.
KEGGimmu:66988.
UCSCiuc008xiy.2. mouse. [Q9CPY7-1]

Organism-specific databases

CTDi51056.
MGIiMGI:1914238. Lap3.

Phylogenomic databases

eggNOGiKOG2597. Eukaryota.
COG0260. LUCA.
GeneTreeiENSGT00530000063255.
HOGENOMiHOG000243129.
HOVERGENiHBG003320.
InParanoidiQ9CPY7.
KOiK11142.
OMAiGIVGMKS.
OrthoDBiEOG091G0ERB.
PhylomeDBiQ9CPY7.
TreeFamiTF314954.

Miscellaneous databases

ChiTaRSiLap3. mouse.
PROiQ9CPY7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039682.
CleanExiMM_LAP3.
ExpressionAtlasiQ9CPY7. baseline and differential.
GenevisibleiQ9CPY7. MM.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPL_MOUSE
AccessioniPrimary (citable) accession number: Q9CPY7
Secondary accession number(s): Q3TFS5
, Q4FJV1, Q4FK15, Q99P44, Q9CWN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.