Reviewed, UniProtKB/Swiss-Prot Q9CPX6 (ATG3_MOUSE)

Names and origin

Protein names

Recommended name:
    Autophagy-related protein 3
Alternative name(s):
    APG3-like

Gene names

Name:	Atg3
Synonyms:	Apg3l

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy By similarity.
Subunit structure	Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5 By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ATG3 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Autophagy-related protein 3		PRO_0000213570
Sites
	Active site	264	1	Glycyl thioester intermediate Potential
Amino acid modifications
	Modified residue	18	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CPX6-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EC5ECACD3247ED66	FASTA	314	35,796
	10 20 30 40 50 60 MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE 70 80 90 100 110 120 LKVKAYLPTD KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT 130 140 150 160 170 180 EAVKEITLES KDSIKLQDCS ALCDEEDEED EGEAADMEEY EESGLLETDE ATLDTRKIVE 190 200 210 220 230 240 ACKAKADAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH 250 260 270 280 290 300 VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV 310 IPTIEYDYTR HFTM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Murine Apg3p is an authentic E2 enzyme for Apg8p homologs." Tanida I., Nishitani T., Tanida-Miyake E., Ueno T., Kominami E. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow, Cerebellum and Colon.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB079386 mRNA. Translation: BAC57452.1. AK005266 mRNA. Translation: BAB23918.1. AK011409 mRNA. Translation: BAB27600.1. AK078877 mRNA. Translation: BAC37437.1. AK150914 mRNA. Translation: BAE29952.1. AK159231 mRNA. Translation: BAE34917.1. BC010809 mRNA. Translation: AAH10809.1.
IPI	IPI00132142.
RefSeq	NP_080678.1.
UniGene	Mm.41775
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q9CPX6.
Proteomic databases
PRIDE	Q9CPX6.
Genome annotation databases
Ensembl	ENSMUSG00000022663. Mus musculus. [Contig view]
GeneID	67841.
KEGG	mmu:67841.
Organism-specific databases
MGI	MGI:1915091. Atg3.
Phylogenomic databases
HOGENOM	Q9CPX6.
HOVERGEN	Q9CPX6.
OMA	Q9CPX6. YHNTGIA.
Gene expression databases
ArrayExpress	Q9CPX6.
Bgee	Q9CPX6.
CleanEx	MM_ATG3.
GermOnline	ENSMUSG00000022663. Mus musculus.
Family and domain databases
InterPro	IPR007135. Autophagy-rel_prot_3. IPR019461. Autophagy-rel_prot_3_C. IPR007134. Autophagy-rel_prot_3_N. [Graphical view]
Pfam	PF03987. Autophagy_act_C. 1 hit. PF10381. Autophagy_Cterm. 1 hit. PF03986. Autophagy_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	325677.
SOURCE	Search...

Entry information

Entry name

ATG3_MOUSE

Accession

Primary (citable) accession number: Q9CPX6
Secondary accession number(s): Q3TXJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)