Q9CPW9 (AMP1D_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase 1D, mitochondrial
EC=3.4.11.18

Alternative name(s):
Methionyl aminopeptidase type 1D, mitochondrial

Gene names

Name:	Metap1d
Synonyms:	Map1d, Metapl1, Mnpepl

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CPW9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9CPW9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion Potential
	Chain	20 – 335	316	Methionine aminopeptidase 1D, mitochondrial		PRO_0000314127
Sites
	Metal binding	178	1	Cobalt 1 By similarity
	Metal binding	189	1	Cobalt 1 By similarity
	Metal binding	189	1	Cobalt 2 By similarity
	Metal binding	252	1	Cobalt 2 By similarity
	Metal binding	284	1	Cobalt 2 By similarity
	Metal binding	315	1	Cobalt 1 By similarity
	Metal binding	315	1	Cobalt 2 By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	259	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 118	118	Missing in isoform 2.		VSP_030210
Experimental info
	Sequence conflict	232	1	N → T in CAC88860. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EF270DDD1826E802	FASTA	335	37,262
	10 20 30 40 50 60 MAAPIGVPLL VRGGCQRILS SPLNHIYLHK RSGSQQRRHF FFWRQRDISH SVVSPAAVSP 70 80 90 100 110 120 AHPVPKRIKK PDYVTTGIVP DWGDSIEVKD EDQIQGLREA CRLARHVLLL AGKSLKVDMT 130 140 150 160 170 180 TEEIDALVHW EIIRHDAYPS PLGYGRFPKS VCTSVNNVLC HGIPDSRPLQ DGDIINIDVT 190 200 210 220 230 240 VYYNGYHGDT SETFLVGNVD ESGKKLVEVA RRCRDEAIAA CRAGAPFSVI GNTISRITHQ 250 260 270 280 290 300 NGLQVCPHFV GHGIGSYFHG HPEIWHHAND NDLPMEEGMA FTIEPIITEG SPEFKVLEDA 310 320 330 WTVVSLDNQR SAQFEHTVLI TPRGVEILTK LPQEA
	Isoform 2 [UniParc]. Checksum: 0EE940FF76A74279	FASTA	217	24,058
	10 20 30 40 50 60 MTTEEIDALV HWEIIRHDAY PSPLGYGRFP KSVCTSVNNV LCHGIPDSRP LQDGDIINID 70 80 90 100 110 120 VTVYYNGYHG DTSETFLVGN VDESGKKLVE VARRCRDEAI AACRAGAPFS VIGNTISRIT 130 140 150 160 170 180 HQNGLQVCPH FVGHGIGSYF HGHPEIWHHA NDNDLPMEEG MAFTIEPIIT EGSPEFKVLE 190 200 210 DAWTVVSLDN QRSAQFEHTV LITPRGVEIL TKLPQEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and expression of mouse MNPEPL peptidase, a novel member of peptidase family M24." Dando P.M., Fortunato M., Rawlings N.D., Barrett A.J. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Spleen.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Cerebellum and Tongue.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ414378 mRNA. Translation: CAC88860.1. AK005241 mRNA. Translation: BAB23899.1. AK010067 mRNA. Translation: BAB26680.1. AL928931 Genomic DNA. Translation: CAM23833.1. BC051534 mRNA. Translation: AAH51534.1.
IPI	IPI00132105. IPI00881819.
RefSeq	NP_079909.1. NM_025633.3.
UniGene	Mm.152796. Mm.443051.
3D structure databases
HSSP	HSSP built from PDB template 2B3H based on UniProtKB P53582.
ProteinModelPortal	Q9CPW9.
SMR	Q9CPW9. Positions 46-332.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9CPW9.
Protein family/group databases
MEROPS	M24.028.
Proteomic databases
PRIDE	Q9CPW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000037210; ENSMUSP00000048190; ENSMUSG00000041921.
GeneID	66559.
KEGG	mmu:66559.
UCSC	uc008kas.1. mouse.
Organism-specific databases
CTD	254042.
MGI	MGI:1913809. Metap1d.
Phylogenomic databases
GeneTree	ENSGT00390000002284.
HOGENOM	HBG299384.
HOVERGEN	HBG067178.
InParanoid	Q9CPW9.
OMA	HIYLHKQ.
OrthoDB	EOG4H9XKZ.
PhylomeDB	Q9CPW9.
Gene expression databases
ArrayExpress	Q9CPW9.
Bgee	Q9CPW9.
CleanEx	MM_METAPL1.
Genevestigator	Q9CPW9.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
KO	K01265.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	322010.
SOURCE	Search...

Entry information

Entry name

AMP1D_MOUSE

Accession

Primary (citable) accession number: Q9CPW9
Secondary accession number(s): Q80WB3, Q91YD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program