Q9CPV9 (P2Y12_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2Y purinoceptor 12
Short name=P2Y12

Gene names

Name:

P2ry12

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 11413167. Source: MGI calcium ion transmembrane transport Inferred from electronic annotation. Source: Compara cell projection organization Inferred from electronic annotation. Source: Compara negative regulation of cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of norepinephrine secretion Inferred from electronic annotation. Source: Compara platelet activation Inferred from mutant phenotype PubMed 11413167. Source: MGI platelet aggregation Inferred from electronic annotation. Source: Compara positive regulation of ion transport Inferred from genetic interaction PubMed 15914557. Source: MGI potassium ion transmembrane transport Inferred from electronic annotation. Source: Compara protein kinase B signaling cascade Inferred from electronic annotation. Source: Compara regulation of calcium ion transport Inferred from electronic annotation. Source: Compara
Cellular_component	basal plasma membrane Inferred from electronic annotation. Source: Compara caveola Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from electronic annotation. Source: Compara integral to plasma membrane Inferred by curator PubMed 11413167. Source: MGI
Molecular_function	ADP receptor activity Inferred from mutant phenotype PubMed 11413167. Source: MGI G-protein coupled adenosine receptor activity Inferred from mutant phenotype PubMed 11413167. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	P2Y purinoceptor 12		PRO_0000070038
Regions
	Topological domain	1 – 31	31	Extracellular Potential
	Transmembrane	32 – 52	21	Helical; Name=1; Potential
	Topological domain	53 – 64	12	Cytoplasmic Potential
	Transmembrane	65 – 85	21	Helical; Name=2; Potential
	Topological domain	86 – 105	20	Extracellular Potential
	Transmembrane	106 – 126	21	Helical; Name=3; Potential
	Topological domain	127 – 148	22	Cytoplasmic Potential
	Transmembrane	149 – 169	21	Helical; Name=4; Potential
	Topological domain	170 – 197	28	Extracellular Potential
	Transmembrane	198 – 218	21	Helical; Name=5; Potential
	Topological domain	219 – 239	21	Cytoplasmic Potential
	Transmembrane	240 – 260	21	Helical; Name=6; Potential
	Topological domain	261 – 287	27	Extracellular Potential
	Transmembrane	288 – 308	21	Helical; Name=7; Potential
	Topological domain	309 – 347	39	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	7	1	N-linked (GlcNAc...) Potential
	Glycosylation	12	1	N-linked (GlcNAc...) Potential
	Disulfide bond	103 ↔ 181		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CPV9 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F107488E57E025F1	FASTA	347	39,474
	10 20 30 40 50 60 MDVPGVNTTS ANTTFSPGTS TLCVRDYKIT QVLFPLLYTV LFFAGLITNS LAMRIFFQIR 70 80 90 100 110 120 SKSNFIIFLK NTVISDLLMI LTFPFKILSD AKLGAGPLRT LVCQVTSVTF YFTMYISISF 130 140 150 160 170 180 LGLITIDRYL KTTRPFKTSS PSNLLGAKIL SVVIWAFMFL ISLPNMILTN RRPKDKDVTK 190 200 210 220 230 240 CSFLKSEFGL VWHEIVNYIC QVIFWINFLI VIVCYSLITK ELYRSYVRTR GSAKVPKKKV 250 260 270 280 290 300 NVKVFIIIAV FFICFVPFHF ARIPYTLSQT RAVFDCSAEN TLFYVKESTL WLTSLNACLD 310 320 330 340 PFIYFFLCKS FRNSLTSMLR CSNSTSTSGT NKKKGQEGGE PSEETPM

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Hippocampus and Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK013804 mRNA. Translation: BAB29000.1. AK014807 mRNA. Translation: BAB29561.1. BC027381 mRNA. Translation: AAH27381.1.
IPI	IPI00132061.
RefSeq	NP_081847.3. NM_027571.3.
UniGene	Mm.422675.
3D structure databases
ProteinModelPortal	Q9CPV9.
SMR	Q9CPV9. Positions 32-319.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000051353.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9CPV9.
Proteomic databases
PaxDb	Q9CPV9.
PRIDE	Q9CPV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050360; ENSMUSP00000051353; ENSMUSG00000036353. ENSMUST00000170388; ENSMUSP00000126819; ENSMUSG00000036353.
GeneID	70839.
KEGG	mmu:70839.
Organism-specific databases
CTD	64805.
MGI	MGI:1918089. P2ry12.
Phylogenomic databases
eggNOG	NOG147554.
GeneTree	ENSGT00640000091213.
HOGENOM	HOG000273884.
HOVERGEN	HBG108228.
InParanoid	Q9CPV9.
KO	K04298.
OMA	IKVFIII.
OrthoDB	EOG4JDH7R.
Gene expression databases
ArrayExpress	Q9CPV9.
Bgee	Q9CPV9.
CleanEx	MM_P2RY12.
Genevestigator	Q9CPV9.
GermOnline	ENSMUSG00000036353. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR002286. P2_purnocptor. IPR005394. P2Y12_purnocptor. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01569. P2Y12PRNCPTR. PR01157. P2YPURNOCPTR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	332388.
SOURCE	Search...

Entry information

Entry name

P2Y12_MOUSE

Accession

Primary (citable) accession number: Q9CPV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program