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Protein

P2Y purinoceptor 12

Gene

P2ry12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Required for normal platelet aggregation and blood coagulation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991ADPBy similarity
Binding sitei103 – 1031ADP; via carbonyl oxygenBy similarity
Binding sitei111 – 1111ADPBy similarity
Binding sitei193 – 1931ADPBy similarity
Binding sitei197 – 1971ADPBy similarity
Binding sitei269 – 2691ADPBy similarity
Binding sitei286 – 2861ADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi162 – 1654ADPBy similarity
Nucleotide bindingi181 – 1855ADPBy similarity
Nucleotide bindingi262 – 2654ADPBy similarity

GO - Molecular functioni

  1. ADP receptor activity Source: MGI
  2. G-protein coupled adenosine receptor activity Source: MGI

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
  2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: MGI
  3. calcium ion transmembrane transport Source: Ensembl
  4. cell projection organization Source: Ensembl
  5. cellular response to organic cyclic compound Source: MGI
  6. G-protein coupled purinergic nucleotide receptor signaling pathway Source: MGI
  7. G-protein coupled receptor signaling pathway Source: MGI
  8. negative regulation of cell differentiation Source: Ensembl
  9. negative regulation of norepinephrine secretion Source: Ensembl
  10. platelet activation Source: MGI
  11. platelet aggregation Source: UniProtKB
  12. positive regulation of ion transport Source: MGI
  13. potassium ion transmembrane transport Source: Ensembl
  14. protein kinase B signaling Source: Ensembl
  15. regulation of calcium ion transport Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Blood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiREACT_326987. P2Y receptors.
REACT_330925. ADP signalling through P2Y purinoceptor 12.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
P2Y purinoceptor 12
Short name:
P2Y12
Gene namesi
Name:P2ry12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1918089. P2ry12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333ExtracellularBy similarityAdd
BLAST
Transmembranei34 – 5623Helical; Name=1By similarityAdd
BLAST
Topological domaini57 – 6711CytoplasmicBy similarityAdd
BLAST
Transmembranei68 – 8821Helical; Name=2By similarityAdd
BLAST
Topological domaini89 – 10315ExtracellularBy similarityAdd
BLAST
Transmembranei104 – 12421Helical; Name=3By similarityAdd
BLAST
Topological domaini125 – 14824CytoplasmicBy similarityAdd
BLAST
Transmembranei149 – 16820Helical; Name=4By similarityAdd
BLAST
Topological domaini169 – 19123ExtracellularBy similarityAdd
BLAST
Transmembranei192 – 21322Helical; Name=5By similarityAdd
BLAST
Topological domaini214 – 23926CytoplasmicBy similarityAdd
BLAST
Transmembranei240 – 26526Helical; Name=6By similarityAdd
BLAST
Topological domaini266 – 28419ExtracellularBy similarityAdd
BLAST
Transmembranei285 – 30420Helical; Name=7By similarityAdd
BLAST
Topological domaini305 – 34743CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. basal plasma membrane Source: Ensembl
  2. caveola Source: Ensembl
  3. cell surface Source: MGI
  4. external side of plasma membrane Source: Ensembl
  5. integral component of plasma membrane Source: GO_Central
  6. intrinsic component of membrane Source: UniProtKB
  7. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, but display impaired blood coagulation, due to defects in platelet aggregation and thrombus formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347P2Y purinoceptor 12PRO_0000070038Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi7 – 71N-linked (GlcNAc...)Sequence Analysis
Glycosylationi12 – 121N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi23 ↔ 276PROSITE-ProRule annotation
Modified residuei61 – 611PhosphoserineBy similarity
Modified residuei63 – 631PhosphoserineBy similarity
Disulfide bondi103 ↔ 181PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9CPV9.
PaxDbiQ9CPV9.
PRIDEiQ9CPV9.

PTM databases

PhosphoSiteiQ9CPV9.

Expressioni

Gene expression databases

BgeeiQ9CPV9.
CleanExiMM_P2RY12.
ExpressionAtlasiQ9CPV9. baseline and differential.
GenevestigatoriQ9CPV9.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051353.

Structurei

3D structure databases

ProteinModelPortaliQ9CPV9.
SMRiQ9CPV9. Positions 21-229, 245-318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The transmembrane domain is composed of seven transmembrane helices; most of these are not strictly perpendicular to the plane of the membrane, but are tilted and/or kinked. Agonist binding promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. Antagonists can bind to an overlapping site, but block the inward movement of the transmembrane helices (By similarity).By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG147554.
GeneTreeiENSGT00640000091213.
HOGENOMiHOG000273884.
HOVERGENiHBG108228.
InParanoidiQ9CPV9.
KOiK04298.
OMAiIKVFIII.
OrthoDBiEOG7C2R1H.
PhylomeDBiQ9CPV9.
TreeFamiTF330969.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005394. P2Y12_rcpt.
[Graphical view]
PANTHERiPTHR24233:SF0. PTHR24233:SF0. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01569. P2Y12PRNCPTR.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CPV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVPGVNTTS ANTTFSPGTS TLCVRDYKIT QVLFPLLYTV LFFAGLITNS
60 70 80 90 100
LAMRIFFQIR SKSNFIIFLK NTVISDLLMI LTFPFKILSD AKLGAGPLRT
110 120 130 140 150
LVCQVTSVTF YFTMYISISF LGLITIDRYL KTTRPFKTSS PSNLLGAKIL
160 170 180 190 200
SVVIWAFMFL ISLPNMILTN RRPKDKDVTK CSFLKSEFGL VWHEIVNYIC
210 220 230 240 250
QVIFWINFLI VIVCYSLITK ELYRSYVRTR GSAKVPKKKV NVKVFIIIAV
260 270 280 290 300
FFICFVPFHF ARIPYTLSQT RAVFDCSAEN TLFYVKESTL WLTSLNACLD
310 320 330 340
PFIYFFLCKS FRNSLTSMLR CSNSTSTSGT NKKKGQEGGE PSEETPM
Length:347
Mass (Da):39,474
Last modified:June 1, 2001 - v1
Checksum:iF107488E57E025F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013804 mRNA. Translation: BAB29000.1.
AK014807 mRNA. Translation: BAB29561.1.
BC027381 mRNA. Translation: AAH27381.1.
CCDSiCCDS17373.1.
RefSeqiNP_081847.3. NM_027571.3.
XP_006502146.1. XM_006502083.2.
XP_006502147.1. XM_006502084.2.
XP_006502148.1. XM_006502085.2.
UniGeneiMm.422675.

Genome annotation databases

EnsembliENSMUST00000050360; ENSMUSP00000051353; ENSMUSG00000036353.
ENSMUST00000170388; ENSMUSP00000126819; ENSMUSG00000036353.
GeneIDi70839.
KEGGimmu:70839.
UCSCiuc008pir.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013804 mRNA. Translation: BAB29000.1.
AK014807 mRNA. Translation: BAB29561.1.
BC027381 mRNA. Translation: AAH27381.1.
CCDSiCCDS17373.1.
RefSeqiNP_081847.3. NM_027571.3.
XP_006502146.1. XM_006502083.2.
XP_006502147.1. XM_006502084.2.
XP_006502148.1. XM_006502085.2.
UniGeneiMm.422675.

3D structure databases

ProteinModelPortaliQ9CPV9.
SMRiQ9CPV9. Positions 21-229, 245-318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051353.

Chemistry

GuidetoPHARMACOLOGYi328.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9CPV9.

Proteomic databases

MaxQBiQ9CPV9.
PaxDbiQ9CPV9.
PRIDEiQ9CPV9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050360; ENSMUSP00000051353; ENSMUSG00000036353.
ENSMUST00000170388; ENSMUSP00000126819; ENSMUSG00000036353.
GeneIDi70839.
KEGGimmu:70839.
UCSCiuc008pir.2. mouse.

Organism-specific databases

CTDi64805.
MGIiMGI:1918089. P2ry12.

Phylogenomic databases

eggNOGiNOG147554.
GeneTreeiENSGT00640000091213.
HOGENOMiHOG000273884.
HOVERGENiHBG108228.
InParanoidiQ9CPV9.
KOiK04298.
OMAiIKVFIII.
OrthoDBiEOG7C2R1H.
PhylomeDBiQ9CPV9.
TreeFamiTF330969.

Enzyme and pathway databases

ReactomeiREACT_326987. P2Y receptors.
REACT_330925. ADP signalling through P2Y purinoceptor 12.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi332388.
PROiQ9CPV9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CPV9.
CleanExiMM_P2RY12.
ExpressionAtlasiQ9CPV9. baseline and differential.
GenevestigatoriQ9CPV9.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005394. P2Y12_rcpt.
[Graphical view]
PANTHERiPTHR24233:SF0. PTHR24233:SF0. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01569. P2Y12PRNCPTR.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Hippocampus and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "P2Y12 regulates platelet adhesion/activation, thrombus growth, and thrombus stability in injured arteries."
    Andre P., Delaney S.M., LaRocca T., Vincent D., DeGuzman F., Jurek M., Koller B., Phillips D.R., Conley P.B.
    J. Clin. Invest. 112:398-406(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiP2Y12_MOUSE
AccessioniPrimary (citable) accession number: Q9CPV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 2001
Last modified: April 1, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.