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Protein

F-box only protein 32

Gene

Fbxo32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 32
Alternative name(s):
Atrogin-1
Muscle atrophy F-box protein
Short name:
MAFbx
Gene namesi
Name:Fbxo32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1914981. Fbxo32.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between cytoplasm and the nucleus.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • SCF ubiquitin ligase complex Source: MGI
  • Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001199231 – 355F-box only protein 32Add BLAST355

Proteomic databases

PaxDbiQ9CPU7.
PeptideAtlasiQ9CPU7.
PRIDEiQ9CPU7.

PTM databases

PhosphoSitePlusiQ9CPU7.

Expressioni

Tissue specificityi

Specifically expressed in cardiac and skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000022358.
CleanExiMM_FBXO32.
GenevisibleiQ9CPU7. MM.

Interactioni

Subunit structurei

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32.By similarity

Protein-protein interaction databases

BioGridi212401. 155 interactors.
IntActiQ9CPU7. 2 interactors.
STRINGi10090.ENSMUSP00000022986.

Structurei

3D structure databases

ProteinModelPortaliQ9CPU7.
SMRiQ9CPU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini223 – 271F-boxAdd BLAST49

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi62 – 67Nuclear localization signalBy similarity6
Motifi169 – 173Nuclear export signalBy similarity5
Motifi280 – 295Bipartite nuclear localization signalBy similarityAdd BLAST16

Sequence similaritiesi

Contains 1 F-box domain.Curated

Phylogenomic databases

eggNOGiKOG3926. Eukaryota.
ENOG410YPWK. LUCA.
GeneTreeiENSGT00390000004915.
HOGENOMiHOG000263478.
HOVERGENiHBG051572.
InParanoidiQ9CPU7.
KOiK10305.
OMAiHFTDRQI.
OrthoDBiEOG091G0M92.
PhylomeDBiQ9CPU7.
TreeFamiTF313070.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CPU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFLGQDWRS PGQSWVKTAD GWKRFLDEKS GSFVSDLSSY CNKEVYSKEN
60 70 80 90 100
LFSSLNYDVA AKKRKKDIQN SKTKTQYFHQ EKWIYVHKGS TKERHGYCTL
110 120 130 140 150
GEAFNRLDFS TAILDSRRFN YVVRLLELIA KSQLTSLSGI AQKNFMNILE
160 170 180 190 200
KVVLKVLEDQ QNIRLIRELL QTLYTSLCTL VQRVGKSVLV GNINMWVYRM
210 220 230 240 250
ETILHWQQQL NSIQISRPAF KGLTITDLPV CLQLNIMQRL SDGRDLVSLG
260 270 280 290 300
QAAPDLHVLS EDRLLWKRLC QYHFSERQIR KRLILSDKGQ LDWKKMYFKL
310 320 330 340 350
VRCYPRREQY GVTLQLCKHC HILSWKGTDH PCTANNPESC SVSLSPQDFI

NLFKF
Length:355
Mass (Da):41,504
Last modified:June 1, 2001 - v1
Checksum:iF464997EB97909A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF441120 mRNA. Translation: AAL49563.1.
AK014793 mRNA. Translation: BAB29555.1.
AK014612 mRNA. Translation: BAB29463.1.
BC027211 mRNA. Translation: AAH27211.1.
CCDSiCCDS27491.1.
RefSeqiNP_080622.1. NM_026346.3.
UniGeneiMm.25683.

Genome annotation databases

EnsembliENSMUST00000022986; ENSMUSP00000022986; ENSMUSG00000022358.
GeneIDi67731.
KEGGimmu:67731.
UCSCiuc007vtk.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF441120 mRNA. Translation: AAL49563.1.
AK014793 mRNA. Translation: BAB29555.1.
AK014612 mRNA. Translation: BAB29463.1.
BC027211 mRNA. Translation: AAH27211.1.
CCDSiCCDS27491.1.
RefSeqiNP_080622.1. NM_026346.3.
UniGeneiMm.25683.

3D structure databases

ProteinModelPortaliQ9CPU7.
SMRiQ9CPU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212401. 155 interactors.
IntActiQ9CPU7. 2 interactors.
STRINGi10090.ENSMUSP00000022986.

PTM databases

PhosphoSitePlusiQ9CPU7.

Proteomic databases

PaxDbiQ9CPU7.
PeptideAtlasiQ9CPU7.
PRIDEiQ9CPU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022986; ENSMUSP00000022986; ENSMUSG00000022358.
GeneIDi67731.
KEGGimmu:67731.
UCSCiuc007vtk.3. mouse.

Organism-specific databases

CTDi114907.
MGIiMGI:1914981. Fbxo32.

Phylogenomic databases

eggNOGiKOG3926. Eukaryota.
ENOG410YPWK. LUCA.
GeneTreeiENSGT00390000004915.
HOGENOMiHOG000263478.
HOVERGENiHBG051572.
InParanoidiQ9CPU7.
KOiK10305.
OMAiHFTDRQI.
OrthoDBiEOG091G0M92.
PhylomeDBiQ9CPU7.
TreeFamiTF313070.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ9CPU7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022358.
CleanExiMM_FBXO32.
GenevisibleiQ9CPU7. MM.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFBX32_MOUSE
AccessioniPrimary (citable) accession number: Q9CPU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.