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Protein

F-box only protein 32

Gene

Fbxo32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • cellular response to dexamethasone stimulus Source: MGI
  • protein ubiquitination Source: UniProtKB-UniPathway
  • response to denervation involved in regulation of muscle adaptation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 32
Alternative name(s):
Atrogin-1
Muscle atrophy F-box protein
Short name:
MAFbx
Gene namesi
Name:Fbxo32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1914981. Fbxo32.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between Cytoplasm and the nucleus.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355F-box only protein 32PRO_0000119923Add
BLAST

Proteomic databases

PaxDbiQ9CPU7.
PRIDEiQ9CPU7.

PTM databases

PhosphoSiteiQ9CPU7.

Expressioni

Tissue specificityi

Specifically expressed in cardiac and skeletal muscle.

Gene expression databases

BgeeiQ9CPU7.
CleanExiMM_FBXO32.
GenevisibleiQ9CPU7. MM.

Interactioni

Subunit structurei

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32.By similarity

Protein-protein interaction databases

BioGridi212401. 155 interactions.
IntActiQ9CPU7. 2 interactions.
STRINGi10090.ENSMUSP00000022986.

Structurei

3D structure databases

ProteinModelPortaliQ9CPU7.
SMRiQ9CPU7. Positions 224-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini223 – 27149F-boxAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi62 – 676Nuclear localization signalBy similarity
Motifi169 – 1735Nuclear export signalBy similarity
Motifi280 – 29516Bipartite nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 F-box domain.Curated

Phylogenomic databases

eggNOGiKOG3926. Eukaryota.
ENOG410YPWK. LUCA.
GeneTreeiENSGT00390000004915.
HOGENOMiHOG000263478.
HOVERGENiHBG051572.
InParanoidiQ9CPU7.
KOiK10305.
OMAiHFTDRQI.
OrthoDBiEOG75J0Q2.
PhylomeDBiQ9CPU7.
TreeFamiTF313070.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CPU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFLGQDWRS PGQSWVKTAD GWKRFLDEKS GSFVSDLSSY CNKEVYSKEN
60 70 80 90 100
LFSSLNYDVA AKKRKKDIQN SKTKTQYFHQ EKWIYVHKGS TKERHGYCTL
110 120 130 140 150
GEAFNRLDFS TAILDSRRFN YVVRLLELIA KSQLTSLSGI AQKNFMNILE
160 170 180 190 200
KVVLKVLEDQ QNIRLIRELL QTLYTSLCTL VQRVGKSVLV GNINMWVYRM
210 220 230 240 250
ETILHWQQQL NSIQISRPAF KGLTITDLPV CLQLNIMQRL SDGRDLVSLG
260 270 280 290 300
QAAPDLHVLS EDRLLWKRLC QYHFSERQIR KRLILSDKGQ LDWKKMYFKL
310 320 330 340 350
VRCYPRREQY GVTLQLCKHC HILSWKGTDH PCTANNPESC SVSLSPQDFI

NLFKF
Length:355
Mass (Da):41,504
Last modified:June 1, 2001 - v1
Checksum:iF464997EB97909A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF441120 mRNA. Translation: AAL49563.1.
AK014793 mRNA. Translation: BAB29555.1.
AK014612 mRNA. Translation: BAB29463.1.
BC027211 mRNA. Translation: AAH27211.1.
CCDSiCCDS27491.1.
RefSeqiNP_080622.1. NM_026346.3.
UniGeneiMm.25683.

Genome annotation databases

EnsembliENSMUST00000022986; ENSMUSP00000022986; ENSMUSG00000022358.
GeneIDi67731.
KEGGimmu:67731.
UCSCiuc007vtk.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF441120 mRNA. Translation: AAL49563.1.
AK014793 mRNA. Translation: BAB29555.1.
AK014612 mRNA. Translation: BAB29463.1.
BC027211 mRNA. Translation: AAH27211.1.
CCDSiCCDS27491.1.
RefSeqiNP_080622.1. NM_026346.3.
UniGeneiMm.25683.

3D structure databases

ProteinModelPortaliQ9CPU7.
SMRiQ9CPU7. Positions 224-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212401. 155 interactions.
IntActiQ9CPU7. 2 interactions.
STRINGi10090.ENSMUSP00000022986.

PTM databases

PhosphoSiteiQ9CPU7.

Proteomic databases

PaxDbiQ9CPU7.
PRIDEiQ9CPU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022986; ENSMUSP00000022986; ENSMUSG00000022358.
GeneIDi67731.
KEGGimmu:67731.
UCSCiuc007vtk.3. mouse.

Organism-specific databases

CTDi114907.
MGIiMGI:1914981. Fbxo32.

Phylogenomic databases

eggNOGiKOG3926. Eukaryota.
ENOG410YPWK. LUCA.
GeneTreeiENSGT00390000004915.
HOGENOMiHOG000263478.
HOVERGENiHBG051572.
InParanoidiQ9CPU7.
KOiK10305.
OMAiHFTDRQI.
OrthoDBiEOG75J0Q2.
PhylomeDBiQ9CPU7.
TreeFamiTF313070.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9CPU7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CPU7.
CleanExiMM_FBXO32.
GenevisibleiQ9CPU7. MM.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy."
    Gomes M.D., Lecker S.H., Jagoe R.T., Navon A., Goldberg A.L.
    Proc. Natl. Acad. Sci. U.S.A. 98:14440-14445(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head and Skin.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiFBX32_MOUSE
AccessioniPrimary (citable) accession number: Q9CPU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.