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Protein

Non-structural maintenance of chromosomes element 3 homolog

Gene

Nsmce3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components (By similarity). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2s) at the E3:substrate complex (By similarity). May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Growth regulation, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural maintenance of chromosomes element 3 homolog
Short name:
Non-SMC element 3 homolog
Alternative name(s):
MAGE-G1 antigen
Necdin-like protein 2
Gene namesi
Name:Nsmce3
Synonyms:Mageg1, Ndnl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913897. Ndnl2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication
  • Chromosometelomere By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001567341 – 279Non-structural maintenance of chromosomes element 3 homologAdd BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9CPR8.
MaxQBiQ9CPR8.
PaxDbiQ9CPR8.
PRIDEiQ9CPR8.

PTM databases

iPTMnetiQ9CPR8.
PhosphoSitePlusiQ9CPR8.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

BgeeiENSMUSG00000070520.
CleanExiMM_NDNL2.
GenevisibleiQ9CPR8. MM.

Interactioni

Subunit structurei

Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3. NSMCE1, NSMCE4A or EID3 and NSMCE3 probably form a subcomplex that bridges the head domains of the SMC5:SMC6 heterodimer. Interacts with PJA1 (By similarity). Binds the C-terminus of E2F1 and NGFR.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
E2f1Q615015EBI-5529102,EBI-1025536
NGFRP081383EBI-5529102,EBI-1387782From a different organism.

Protein-protein interaction databases

BioGridi211620. 2 interactors.
IntActiQ9CPR8. 4 interactors.
MINTiMINT-4101061.
STRINGi10090.ENSMUSP00000091889.

Structurei

3D structure databases

ProteinModelPortaliQ9CPR8.
SMRiQ9CPR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 259MAGEPROSITE-ProRule annotationAdd BLAST201

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 279Interaction with NSMCE1By similarityAdd BLAST228

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOVERGENiHBG006315.
InParanoidiQ9CPR8.
OMAiTFVKQQY.
OrthoDBiEOG091G0TJ0.
TreeFamiTF328505.

Family and domain databases

InterProiIPR002190. MHD_dom.
[Graphical view]
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CPR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQKPRGRGR PSTQADPERD WGGAGEEGPS TSRAAGGSSQ GSRASLSAPT
60 70 80 90 100
VGPRTQKQLE LKVAELVQFL LIKDQKKIPI KRTDILKHVV GDYRDVYPNL
110 120 130 140 150
LKLAAERLQY VFGYKLVELE PKSHSYILIN MLEPVEADAE MRGDQGTPIS
160 170 180 190 200
GLLMIVLGLI FMKGNTITET EVWDFLRRLG VYPTKKHLIF GDPKKLITED
210 220 230 240 250
FVRQRYLEYR RIPHTDPVDY ELQWGPRTNL ETSKMKVLKF VAKVHNQDPK
260 270
DWPTQYCEAL ADEESRARPA TASAPATSS
Length:279
Mass (Da):31,460
Last modified:June 1, 2001 - v1
Checksum:iFE2435919BD63160
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36G → A in BAB31133 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319979 mRNA. Translation: AAK01207.1.
AK010294 mRNA. Translation: BAB26830.1.
AK017727 mRNA. Translation: BAB30899.1.
AK018250 mRNA. Translation: BAB31133.1.
AK049759 mRNA. Translation: BAC33907.1.
AK076471 mRNA. Translation: BAC36358.1.
BC034892 mRNA. Translation: AAH34892.1.
BC092289 mRNA. Translation: AAH92289.2.
CCDSiCCDS21337.1.
RefSeqiNP_075728.1. NM_023239.4.
UniGeneiMm.19944.

Genome annotation databases

EnsembliENSMUST00000094331; ENSMUSP00000091889; ENSMUSG00000070520.
GeneIDi66647.
KEGGimmu:66647.
UCSCiuc009hgm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319979 mRNA. Translation: AAK01207.1.
AK010294 mRNA. Translation: BAB26830.1.
AK017727 mRNA. Translation: BAB30899.1.
AK018250 mRNA. Translation: BAB31133.1.
AK049759 mRNA. Translation: BAC33907.1.
AK076471 mRNA. Translation: BAC36358.1.
BC034892 mRNA. Translation: AAH34892.1.
BC092289 mRNA. Translation: AAH92289.2.
CCDSiCCDS21337.1.
RefSeqiNP_075728.1. NM_023239.4.
UniGeneiMm.19944.

3D structure databases

ProteinModelPortaliQ9CPR8.
SMRiQ9CPR8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211620. 2 interactors.
IntActiQ9CPR8. 4 interactors.
MINTiMINT-4101061.
STRINGi10090.ENSMUSP00000091889.

PTM databases

iPTMnetiQ9CPR8.
PhosphoSitePlusiQ9CPR8.

Proteomic databases

EPDiQ9CPR8.
MaxQBiQ9CPR8.
PaxDbiQ9CPR8.
PRIDEiQ9CPR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094331; ENSMUSP00000091889; ENSMUSG00000070520.
GeneIDi66647.
KEGGimmu:66647.
UCSCiuc009hgm.1. mouse.

Organism-specific databases

CTDi66647.
MGIiMGI:1913897. Ndnl2.

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOVERGENiHBG006315.
InParanoidiQ9CPR8.
OMAiTFVKQQY.
OrthoDBiEOG091G0TJ0.
TreeFamiTF328505.

Miscellaneous databases

PROiQ9CPR8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070520.
CleanExiMM_NDNL2.
GenevisibleiQ9CPR8. MM.

Family and domain databases

InterProiIPR002190. MHD_dom.
[Graphical view]
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSE3_MOUSE
AccessioniPrimary (citable) accession number: Q9CPR8
Secondary accession number(s): Q569V7, Q9D378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.