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Reviewed, UniProtKB/Swiss-Prot Q9CPL4 (NRFA_PASMU)

Last modified November 3, 2009. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: PM0023
OrganismPasteurella multocida [Complete proteome] [HAMAP]
Taxonomic identifier747 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

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Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity.

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity.

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5050 Potential
Chain51 – 510460Cytochrome c-552 HAMAP MF_01182
PRO_0000006580

Sites

Metal binding1241Iron (heme 3 axial ligand) By similarity
Metal binding1561Iron (heme 1 axial ligand) By similarity
Metal binding1941Iron (heme 2 axial ligand) By similarity
Metal binding2431Iron (heme 3 axial ligand) By similarity
Metal binding2451Calcium By similarity
Metal binding2461Calcium; via carbonyl oxygen By similarity
Metal binding2911Calcium; via carbonyl oxygen By similarity
Metal binding2931Calcium By similarity
Metal binding3051Iron (heme 5 axial ligand) By similarity
Metal binding3161Iron (heme 4 axial ligand) By similarity
Metal binding3311Iron (heme 2 axial ligand) By similarity
Metal binding3481Iron (heme 5 axial ligand) By similarity
Metal binding4231Iron (heme 4 axial ligand) By similarity
Binding site1521Heme 1 (covalent) By similarity
Binding site1551Heme 1 (covalent) By similarity
Binding site1901Heme 2 (covalent) By similarity
Binding site1931Heme 2 (covalent) By similarity
Binding site2391Heme 3 (covalent) By similarity
Binding site2421Heme 3 (covalent) By similarity
Binding site2461Substrate By similarity
Binding site2941Substrate By similarity
Binding site3121Heme 4 (covalent) By similarity
Binding site3151Heme 4 (covalent) By similarity
Binding site3441Heme 5 (covalent) By similarity
Binding site3471Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9CPL4-1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 40A2695C513DBC43

FASTA51057,260
        10         20         30         40         50         60 
MVVFLFILYR QSDLKFKSMN GVIIVNTLKK RLFVATTMIW GLSVTLPVLA EYKPIEQPVE 

        70         80         90        100        110        120 
PANPSLKIES RNDLFKEKYP KQYQTWADTS KSTDLDSVNY EDPRVIVLWA GYAFAKDYKK 

       130        140        150        160        170        180 
PRGHFYAVTD VREILRTAAP MTPDAGPMPM ACWSCKSPDV PRLIAERGEA GYFGATWASG 

       190        200        210        220        230        240 
GSEVVNPIGC ADCHDTTSKD FAEGKPALRI ARPYVLRALE KIDHKFDTSD RTDQRAALCA 

       250        260        270        280        290        300 
NCHVEYYFAG DLKQVTFPWD NGITVDAMEK YYDDIGFVDW THAVSKAPML KAQHPDYETW 

       310        320        330        340        350        360 
MLGVHGKNGV TCIDCHMPKV QGADGKVYTD HQIGNPFNAF EHTCANCHDQ SKEKLQAMVK 

       370        380        390        400        410        420 
SRKTEIKDVM LRLEDQLVAA HFEAKAAWEA GATKEEMKEA LQDIRHAQWR WDYAAAGHGG 

       430        440        450        460        470        480 
HIHAPDVLLK VIGTGLDKSS DARTKLVRVL AKHGITDPVQ LPDISTAENA WKATGVDIEK 

       490        500        510 
ERKAKAEFLK TVVPQWDKEA REKGLLPAEK

« Hide

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References

[1]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.

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Cross-references

Sequence databases

AE004439 Genomic DNA. Translation: AAK02107.1.
RefSeqNP_244960.1.

3D structure databases

HSSPHSSP built from PDB template 1GU6 based on UniProtKB P32050.
ModBaseSearch...

Genome annotation databases

GeneID1243370.
GenomeReviewsGene locus PM0023 in contig AE004439_GR.
KEGGpmu:PM0023.
NMPDRfig|272843.1.peg.23.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ9CPL4.
OMACHDQSKE.

Enzyme and pathway databases

BRENDA1.7.2.2. 258935.

Family and domain databases

HAMAPMF_01182.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_PASMU
AccessionPrimary (citable) accession number: Q9CPL4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: June 1, 2001
Last modified: November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents