Q9CPI4 (MEND_PASMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Short name=SEPHCHC synthase
EC=2.2.1.9

Alternative name(s):
Menaquinone biosynthesis protein MenD

Gene names

Name:	menD
Ordered Locus Names:	PM0054

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP MF_01659
Catalytic activity	Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP MF_01659
Cofactor	Magnesium or manganese By similarity. HAMAP MF_01659 Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP MF_01659
Subunit structure	Homodimer By similarity. HAMAP MF_01659
Sequence similarities	Belongs to the TPP enzyme family. MenD subfamily.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Ligand	Magnesium Manganese Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP MF_01659		PRO_0000090831

Sequences

	Sequence		Length	Mass (Da)
	Q9CPI4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F1ED881B15E6A95A	FASTA	568	63,377
	10 20 30 40 50 60 MSISVFNRCW SKVILETLVR QGVAHFCIAP GSRSTPLTLE AIRLQDSHRV TCHTHFDERG 70 80 90 100 110 120 LGFFALGLAK VSKKPVAVIV TSGTAAANLY PAIIEARQTG VNLIVLTADR PPELIECGAN 130 140 150 160 170 180 QAIVQPHMYA DYPVASVNLP RPSQDYSADW LISRLEQACY QQARQPGVVH VNVPFAEPLY 190 200 210 220 230 240 EASETDIEAH SWLVPIQRWL NQHKPWIQQQ TDQKEVLMHA NWDHWRTMRG VIVVGRLPVE 250 260 270 280 290 300 QTMGIMAWAN TMGWIALTDV QSSVEASLPY ADIWLANQTV KQKLLQADIV IQFGSGFISK 310 320 330 340 350 360 RVNQFLAAFK QEYWIVEQST GLIDPNHHAH TRFNAKVHHW LRAHPPLRQK PWLLEPLALS 370 380 390 400 410 420 KFCASFIEKQ VGGNLNEASL AHHLDRILSN NGVLFLGNSL FVRLVDALAK LPEGYPVYTN 430 440 450 460 470 480 RGASGIDGLL ATAAGIGIGA NQPLVAMIGD VSALYDINSL ALFKKVTQPT IIFVINNNGG 490 500 510 520 530 540 AIFDMLPVEA DVKEKYYRMP HHLEFSQLVQ TFDLKYARPY TWADLGAVLK VAYSRRETTL 550 560 IEIKVGASDA STIYKRLIEQ ISYAVIGE

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK02138.1.
RefSeq	NP_244991.1. NC_002663.1.
3D structure databases
ProteinModelPortal	Q9CPI4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1243401.
GenomeReviews	Gene locus PM0054 in contig AE004439_GR.
KEGG	pmu:PM0054.
NMPDR	fig|272843.1.peg.54.
PATRIC	22869240. VBIPasMul88067_0055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG634405.
OMA	ICTSGTA.
ProtClustDB	PRK07449.
Enzyme and pathway databases
BioCyc	PMUL272843:PM0054-MONOMER.
Family and domain databases
HAMAP	MF_01659. MenD. [Tree]
InterPro	IPR004433. MenaQ_synth_MenD. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K02551.
PANTHER	PTHR18968:SF3. PTHR18968:SF3. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PIRSF	PIRSF004983. MenD. 1 hit.
TIGRFAMs	TIGR00173. MenD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MEND_PASMU

Accession

Primary (citable) accession number: Q9CPI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program