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Q9CP53

- TOP3_PASMU

UniProt

Q9CP53 - TOP3_PASMU

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Protein

DNA topoisomerase 3

Gene
topB, PM0207
Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Magnesium 1; catalytic By similarity
Sitei61 – 611Interaction with DNA By similarity
Metal bindingi103 – 1031Magnesium 1; catalytic By similarity
Metal bindingi103 – 1031Magnesium 2 By similarity
Metal bindingi105 – 1051Magnesium 2 By similarity
Sitei170 – 1701Interaction with DNA By similarity
Sitei178 – 1781Interaction with DNA By similarity
Sitei185 – 1851Interaction with DNA By similarity
Active sitei342 – 3421O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei344 – 3441Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-210-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III
Gene namesi
Name:topB
Ordered Locus Names:PM0207
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650DNA topoisomerase 3UniRule annotationPRO_0000145188Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272843.PM0207.

Structurei

3D structure databases

ProteinModelPortaliQ9CP53.
SMRiQ9CP53. Positions 1-617.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 134134ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1996Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG0550.
HOGENOMiHOG000086848.
KOiK03169.
OMAiFCPDAEY.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CP53-1 [UniParc]FASTAAdd to Basket

« Hide

MRLFIAEKPS LARAIADVLP KPHQRGDGFI KCGADDYVTW CIGHLLEQAE    50
PDAYDPKFKQ WRLEHLPIIP EKWQLIPRKD VHKQLTIVEK LIHQADILIN 100
AGDPDREGQL LVDEVFSYAN LSVDKLNQIQ RCLISDLNPS AVEKAVNKLQ 150
SNRNFIPLAT SALARARADW LYGINMTRAY TLRGRQAGYQ GVLSVGRVQT 200
PVLGLIVRRD LEIENFKPQD FFEVLAHIQA ETPEKITALS AQEKANIPQF 250
KALWQPSKAC EDYQDEEGRV LSLGLVENVV KRIAQQPAEV VEYVDKREHE 300
SAPLPYSLSA LQIDAAKRYG LSAQEVLDIC QRLYETHRLI TYPRSDCRYL 350
PEEHFGERTK VFQAISRHIS DYQPLPDILN PEQKNRCWND KKVEAHHAII 400
PTAKNTPVNL NQREWQIYHL IARQYLMQFC PDAEYRKSKI TLNIAGGTFI 450
AQARNLQVAG WKQLLGKEDS DEQQEPLLPV VKKGQILFCE KGEIVSKKTQ 500
PPKPFTDATL LSAMTGIARF VQDKELKKIL RETDGLGTEA TRAGIIELLF 550
KRGFLYKKGR NIHSTETGRI LIQALPDVAT QPDMTAHWES QLTSISQKEM 600
SYQQFMSTLT NFLPELMRYV NFAALRQLSQ VEKPQSFSKK MSAKSKKRPT 650
Length:650
Mass (Da):74,196
Last modified:June 1, 2001 - v1
Checksum:iAE779A388CC7D166
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK02291.1.
RefSeqiNP_245144.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK02291; AAK02291; PM0207.
GeneIDi1243554.
KEGGipmu:PM0207.
PATRICi22869560. VBIPasMul88067_0213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK02291.1 .
RefSeqi NP_245144.1. NC_002663.1.

3D structure databases

ProteinModelPortali Q9CP53.
SMRi Q9CP53. Positions 1-617.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272843.PM0207.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK02291 ; AAK02291 ; PM0207 .
GeneIDi 1243554.
KEGGi pmu:PM0207.
PATRICi 22869560. VBIPasMul88067_0213.

Phylogenomic databases

eggNOGi COG0550.
HOGENOMi HOG000086848.
KOi K03169.
OMAi FCPDAEY.
OrthoDBi EOG60CWM6.

Enzyme and pathway databases

BioCyci PMUL272843:GC8W-210-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00953. Topoisom_3_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01056. topB. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiTOP3_PASMU
AccessioniPrimary (citable) accession number: Q9CP53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi