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Q9CP40 (DSBD_PASMU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thiol:disulfide interchange protein DsbD
EC=1.8.1.8
Alternative name(s):
Protein-disulfide reductase
Short name=Disulfide reductase
Gene names
Name:dsbD
Ordered Locus Names:PM0221
OrganismPasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]
Taxonomic identifier272843 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity. HAMAP MF_00399

Catalytic activity

Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00399.

Sequence similarities

Belongs to the thioredoxin family. DsbD subfamily.

Contains 1 thioredoxin domain.

Sequence caution

The sequence AAK02305.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 576557Thiol:disulfide interchange protein DsbD HAMAP MF_00399
PRO_0000007380

Regions

Topological domain20 – 177158Periplasmic Potential
Transmembrane178 – 19821Helical; Potential
Topological domain199 – 22931Cytoplasmic Potential
Transmembrane230 – 25021Helical; Potential
Topological domain251 – 2533Periplasmic Potential
Transmembrane254 – 27421Helical; Potential
Topological domain275 – 29521Cytoplasmic Potential
Transmembrane296 – 31621Helical; Potential
Topological domain317 – 33620Periplasmic Potential
Transmembrane337 – 35721Helical; Potential
Topological domain358 – 36710Cytoplasmic Potential
Transmembrane368 – 38821Helical; Potential
Topological domain389 – 3946Periplasmic Potential
Transmembrane395 – 41521Helical; Potential
Topological domain416 – 4194Cytoplasmic Potential
Transmembrane420 – 44021Helical; Potential
Topological domain441 – 576136Periplasmic Potential
Domain433 – 570138Thioredoxin

Amino acid modifications

Disulfide bond120 ↔ 125Redox-active By similarity
Disulfide bond193 ↔ 315Redox-active By similarity
Disulfide bond485 ↔ 488Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CP40 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 98507447D5EDFA41

FASTA57664,465
        10         20         30         40         50         60 
MKKIFFIFFL IWTSLSVNAG VFDKKNAFLK AEQAFVFTES QQNDRLSLNW DIASGYYLYK 

        70         80         90        100        110        120 
KSLEIKGENG QLFIPQLPLA EIHQDDFFGD VEIYRHQLTF SLPFTQLSAT KQVEITYQGC 

       130        140        150        160        170        180 
TEGFCYPPET RVIALTSLER ESGQISSAVL KEESVLSELP LAEQDRLAAK LIESKYAVFW 

       190        200        210        220        230        240 
FFLFGLGLAF TPCVLPMLPL LSAIVIGREQ RPSTAKAFGL SVVYVQGMAL TYTLLGLIVA 

       250        260        270        280        290        300 
AIGLPFQVAL QSPYVLLTLS AIFVLLALSM FGLFTLQLPV SLQTKLTQYS QQQKSGVFGG 

       310        320        330        340        350        360 
VFVMGMIAGL VASPCTSAPL SGALLYVAQS GDLFTGAITL YLLALGMGLP LIFITVFGNN 

       370        380        390        400        410        420 
ILPKSGNWMI QVKNAFGFVL LALPIFLISR VFPEIEMPLW FSLAFAFSLW LLYQFRNNKG 

       430        440        450        460        470        480 
VWVFTFILGV MGYLYYSYAM NRHTPAHQTT SLSQFERITS YAQLQQVLSK NPNSLAMLDL 

       490        500        510        520        530        540 
YADWCVACKE FETHTFSDVA VQKAFTDVLL LQVDMTKNSE ANRELMQKLH VIGLPTLIFF 

       550        560        570 
NQQGQEIEGS RITGFMQASP FVDWLQKMKS IQPISQ

« Hide

References

[1]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004439 Genomic DNA. Translation: AAK02305.1. Different initiation.
RefSeqNP_245158.1. NC_002663.1.

3D structure databases

ProteinModelPortalQ9CP40.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1243568.
GenomeReviewsGene locus PM0221 in contig AE004439_GR.
KEGGpmu:PM0221.
NMPDRfig|272843.1.peg.221.
PATRIC22869594. VBIPasMul88067_0229.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG640883.
OMAKPHTDEY.
ProtClustDBPRK00293.

Enzyme and pathway databases

BioCycPMUL272843:PM0221-MONOMER.

Family and domain databases

HAMAPMF_00399. DbsD.
[Tree]
InterProIPR003834. Cyt_c_assmbl_TM_dom.
IPR022910. Thiol_diS_interchange_DbsD.
IPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KOK04084.
PANTHERPTHR10438. Trx. 1 hit.
PfamPF02683. DsbD. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMSSF52833. Thiordxn-like_fd. 1 hit.
PROSITEPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDSBD_PASMU
AccessionPrimary (citable) accession number: Q9CP40
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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