Q9CNX8 (GUAA_PASMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	PM0293

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_0000140155
Regions
	Domain	8 – 205	198	Glutamine amidotransferase type-1
	Domain	206 – 398	193	GMPS ATP-PPase
	Nucleotide binding	233 – 239	7	ATP By similarity
Sites
	Active site	85	1	Nucleophile By similarity
	Active site	179	1	By similarity
	Active site	181	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CNX8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 87850E94775D0467	FASTA	523	58,230
	10 20 30 40 50 60 MNNIHNHKIL ILDFGSQYTQ LIARRVREIG VYCELWAWDV SEADIREFNP TGIILSGGPE 70 80 90 100 110 120 STTEENSPRA PEYVFNAGVP VLGICYGMQT MAMQLGGLTE TSTHREFGYA SVNLKAADAL 130 140 150 160 170 180 FAQLNDDVTS SQPKLDVWMS HGDKVTRLPD HFQVTAMTST CPIAAMSDER RRFYGVQFHP 190 200 210 220 230 240 EVTHTKSGLE LLTNFVVKIC GCERNWTPEN IIEDAVARLK AQVGDDEVIL GLSGGVDSSV 250 260 270 280 290 300 TALLLHRAIG KNLHCVFVDN GLLRLNEAEQ VMEMFGDKFG LNIIHVKAED RFLDALKGID 310 320 330 340 350 360 EPEAKRKMIG KVFVDVFDDE SKKLTSVKWL AQGTIYPDVI ESAASKTGKA HVIKSHHNVG 370 380 390 400 410 420 GLPDYMKLGL VEPLRELFKD EVRKIGLALG LPAEMLNRHP FPGPGLGVRV LGEIKKEYCD 430 440 450 460 470 480 LVRLADAIFM EELHASGWYY KVSQAFTVFL PVKSVGVMGD GRKYDWVVSL RAVETIDFMT 490 500 510 520 AHWAHLPYDL LGKISNRIIN EVNGISRVVY DVSGKPPATI EWE

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK02377.1.
RefSeq	NP_245230.1. NC_002663.1.
3D structure databases
ProteinModelPortal	Q9CNX8.
SMR	Q9CNX8. Positions 3-523.
ModBase	Search...
Proteomic databases
PRIDE	Q9CNX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1243640.
GenomeReviews	Gene locus PM0293 in contig AE004439_GR.
KEGG	pmu:PM0293.
NMPDR	fig|272843.1.peg.293.
PATRIC	22869742. VBIPasMul88067_0303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Enzyme and pathway databases
BioCyc	PMUL272843:PM0293-MONOMER.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_PASMU

Accession

Primary (citable) accession number: Q9CNX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program