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Protein

NAD kinase

Gene

nadK

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841Proton acceptorUniRule annotation
Binding sitei170 – 1701NADUniRule annotation
Binding sitei187 – 1871NADUniRule annotation
Binding sitei189 – 1891NADUniRule annotation
Binding sitei260 – 2601NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 852NADUniRule annotation
Nucleotide bindingi159 – 1602NADUniRule annotation
Nucleotide bindingi200 – 2056NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-343-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:PM0333
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305NAD kinasePRO_0000120644Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272843.PM0333.

Structurei

3D structure databases

ProteinModelPortaliQ9CNU2.
SMRiQ9CNU2. Positions 16-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CNU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHPNTIDIK ALQSSFQIIG LLGKPRHDVT LQMHKNLFQW LLEKGYQVLV
60 70 80 90 100
ERPIGEQLGL SENYLASVDE IGQQAQLAIV IGGDGNVLGR ARTLAKYDIA
110 120 130 140 150
LIGINRGNLG FLTDIDPKNA YSQLQACLED GDCFVEERFI LEASVERNGK
160 170 180 190 200
IIARGNAVNE AVVHPAKIAH MIDFHVYIND KFAFSQRSDG LIISTPTGST
210 220 230 240 250
AYSLSAGGPI LTPQLNAIAL VPMFPHTLSS RPLVIDGDSK ISIRFAEYNT
260 270 280 290 300
SQLEVGCDSQ VALEFSPDDI VHIQKSPDKL RLLHLKNYNY YKVLSSKLGW

LRNSV
Length:305
Mass (Da):33,711
Last modified:May 3, 2001 - v1
Checksum:iBD8BC03D93C41BE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK02417.1.
RefSeqiNP_245270.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK02417; AAK02417; PM0333.
KEGGipmu:PM0333.
PATRICi22869840. VBIPasMul88067_0346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK02417.1.
RefSeqiNP_245270.1. NC_002663.1.

3D structure databases

ProteinModelPortaliQ9CNU2.
SMRiQ9CNU2. Positions 16-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272843.PM0333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK02417; AAK02417; PM0333.
KEGGipmu:PM0333.
PATRICi22869840. VBIPasMul88067_0346.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-343-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiNADK_PASMU
AccessioniPrimary (citable) accession number: Q9CNU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2001
Last sequence update: May 3, 2001
Last modified: March 31, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.