Q9CNL6 (DNAB_PASMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replicative DNA helicase
EC=3.6.4.12

Gene names

Name:	dnaB
Ordered Locus Names:	PM0412

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Replicative DNA helicase		PRO_0000102027
Regions
	Domain	193 – 461	269	SF4 helicase
	Nucleotide binding	224 – 231	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9CNL6 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7BE33C8CA32AE87C	FASTA	467	52,011
	10 20 30 40 50 60 MAKKNAQIPS KPLEQGIVPP HSLEAEQSVL GGILIHPAHW DSISDMLIAD DFYLAAHRVI 70 80 90 100 110 120 FQEMENLLRQ GKPIDLINLY EFLREKNLSE DVGGFAYLAE LSKNTPSVSN IVSYAAIVRD 130 140 150 160 170 180 RAILRDLISV SNDVAQSCYT TKGMEVKDIL DEAERKVFAI SEKRTTSNEG PQNICAILEK 190 200 210 220 230 240 TIDRIELLSK TEGHNGITGV STGFDALDKK TAGLQPSDLI IVAARPSMGK TTFAMNLCEN 250 260 270 280 290 300 AALKSDKPVL VFSLEMPAEQ IMMRSLASLS RVDQTKIRTG QGLDQSDWSK IGSTMGLFAN 310 320 330 340 350 360 KPNLYIDDSS GLTPTELRSR ARRVYRENGG LSLIMVDYLQ LMRAPGFADN RTLEIAEISR 370 380 390 400 410 420 SLKALAKELE VPVIALSQLN RTLENRADKR PVNSDLRESG SIEQDADLIM FIYRDEVYNE 430 440 450 460 NSEDKGVAEI IIGKQRNGPI GRVRLAFRGQ FSRFDNLAEQ RDIRDDY

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK02496.1.
RefSeq	NP_245349.1. NC_002663.1.
3D structure databases
ProteinModelPortal	Q9CNL6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1243759.
GenomeReviews	Gene locus PM0412 in contig AE004439_GR.
KEGG	pmu:PM0412.
NMPDR	fig|272843.1.peg.412.
PATRIC	22869998. VBIPasMul88067_0424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734566.
OMA	IMRMLSS.
Enzyme and pathway databases
BioCyc	PMUL272843:PM0412-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR007692. DNA_helicase_DnaB. IPR007694. DNA_helicase_DnaB-like_C. IPR007693. DNA_helicase_DnaB-like_N. IPR016136. DNA_helicase_N/primase_C. [Graphical view]
Gene3D	G3DSA:1.10.860.10. DnaB_N. 1 hit.
KO	K02314.
Pfam	PF00772. DnaB. 1 hit. PF03796. DnaB_C. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF48024. DNA_helicase_DnaB-like_N. 1 hit.
TIGRFAMs	TIGR00665. DnaB. 1 hit.
PROSITE	PS51199. SF4_HELICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_PASMU

Accession

Primary (citable) accession number: Q9CNL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program