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Q9CN30

- TOP1_PASMU

UniProt

Q9CN30 - TOP1_PASMU

Protein

DNA topoisomerase 1

Gene

topA

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (20 Jun 2001)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
    Sitei33 – 331Interaction with DNAUniRule annotation
    Metal bindingi117 – 1171Magnesium 1; catalyticUniRule annotation
    Metal bindingi117 – 1171Magnesium 2UniRule annotation
    Metal bindingi119 – 1191Magnesium 2UniRule annotation
    Sitei174 – 1741Interaction with DNAUniRule annotation
    Sitei175 – 1751Interaction with DNAUniRule annotation
    Sitei178 – 1781Interaction with DNAUniRule annotation
    Sitei190 – 1901Interaction with DNAUniRule annotation
    Active sitei325 – 3251O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei327 – 3271Interaction with DNAUniRule annotation
    Sitei513 – 5131Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri605 – 63632C4-type 1Add
    BLAST
    Zinc fingeri667 – 69428C4-type 2Add
    BLAST
    Zinc fingeri716 – 73924C4-type 3Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
    4. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciPMUL272843:GC8W-636-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:PM0615
    OrganismiPasteurella multocida (strain Pm70)
    Taxonomic identifieri272843 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
    ProteomesiUP000000809: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 868868DNA topoisomerase 1PRO_0000145160Add
    BLAST

    Proteomic databases

    PRIDEiQ9CN30.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272843.PM0615.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CN30.
    SMRiQ9CN30. Positions 3-598, 748-868.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 148146ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni198 – 2036Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri605 – 63632C4-type 1Add
    BLAST
    Zinc fingeri667 – 69428C4-type 2Add
    BLAST
    Zinc fingeri716 – 73924C4-type 3Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000004018.
    KOiK03168.
    OMAiVVECDKC.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 3 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CN30-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKSLVIVES PAKAKTINKY LGSNYVVKSS VGHIRDLPTV GSSNGEKAKA    50
    ISTKGLSAEE KSAIKQEKDR NALVKRMGID PYHQWKANYQ ILPGKEKVVA 100
    ELKSLAKKAD HIYLATDLDR EGEAIAWHLR EVIGGDDARF SRVVFNEITK 150
    NAIQQAFEKP EQLNLDRVNA QQTRRFLDRV VGFMVSPLLW KKVARGLSAG 200
    RVQSVAVKLV VEREREIKAF QPEEYWEIDV LTQTQAKQAL RLAVTQFKGK 250
    KFEPKNATQA QSAVDFLQHA DYVVSDLETK PTHSRAKAPF ITSTLQQTAS 300
    TRLGFGVKKT MMLAQRLYEA GYITYMRTDS TNLSQDALKM VRGYIQSHYG 350
    ESYLPSKPVF YSSKENAQEA HEAIRPSDVN TLAEQLTGMD KDAVRLYDLI 400
    WRQFVACQMP AAQYDSTTVT VRAGEYELKA KGRILRFDGW TKVLPTMSKN 450
    AEDQVLPDVQ LNEKLHLEHV LPEQLFTKPP ARFSEAALVK ELEKRGIGRP 500
    STYAAIISTI QERGYVRVEN RRFYAEKMGE IVTDRLNQSF TALMNYDFTA 550
    NMENILDQIA NGEKNWKTEL NQFFKDFSQQ LSQAELDELE GGMKPNSLVP 600
    TDINCPTCGR KMAIRTASTG VFLGCSGYAL PPKERCKTTM NLIPEAELLN 650
    VLDEASETKA LMERKRCPKC GTAMDSYLID PERKIHICGH NPNCDGYVLE 700
    QGSFKIKGYD GPIVECDKCG ADMHLKLGRF GKYMGCTSCD NTRKILKSGE 750
    VAPPKEEPTH FPELKCEKSD AYFVLRDGAS GVFMSAHNFP KSRETRAPKV 800
    AELALYRDRL PEKLRYLADA PQHDPEGNDA IVRFSRKEKR QYVTSEKNGK 850
    ATKWIVDYLG NQWVERKK 868
    Length:868
    Mass (Da):98,048
    Last modified:June 20, 2001 - v1
    Checksum:iE63DF724C4B4ED8D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004439 Genomic DNA. Translation: AAK02699.1.
    RefSeqiNP_245552.1. NC_002663.1.

    Genome annotation databases

    EnsemblBacteriaiAAK02699; AAK02699; PM0615.
    GeneIDi1243962.
    KEGGipmu:PM0615.
    PATRICi22870414. VBIPasMul88067_0623.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004439 Genomic DNA. Translation: AAK02699.1 .
    RefSeqi NP_245552.1. NC_002663.1.

    3D structure databases

    ProteinModelPortali Q9CN30.
    SMRi Q9CN30. Positions 3-598, 748-868.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272843.PM0615.

    Proteomic databases

    PRIDEi Q9CN30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK02699 ; AAK02699 ; PM0615 .
    GeneIDi 1243962.
    KEGGi pmu:PM0615.
    PATRICi 22870414. VBIPasMul88067_0623.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000004018.
    KOi K03168.
    OMAi VVECDKC.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci PMUL272843:GC8W-636-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 3 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pm70.

    Entry informationi

    Entry nameiTOP1_PASMU
    AccessioniPrimary (citable) accession number: Q9CN30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: June 20, 2001
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3