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Q9CN30

- TOP1_PASMU

UniProt

Q9CN30 - TOP1_PASMU

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Protein
DNA topoisomerase 1
Gene
topA, PM0615
Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalytic By similarity
Sitei33 – 331Interaction with DNA By similarity
Metal bindingi117 – 1171Magnesium 1; catalytic By similarity
Metal bindingi117 – 1171Magnesium 2 By similarity
Metal bindingi119 – 1191Magnesium 2 By similarity
Sitei174 – 1741Interaction with DNA By similarity
Sitei175 – 1751Interaction with DNA By similarity
Sitei178 – 1781Interaction with DNA By similarity
Sitei190 – 1901Interaction with DNA By similarity
Active sitei325 – 3251O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei327 – 3271Interaction with DNA By similarity
Sitei513 – 5131Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri605 – 63632C4-type 1UniRule annotation
Add
BLAST
Zinc fingeri667 – 69428C4-type 2UniRule annotation
Add
BLAST
Zinc fingeri716 – 73924C4-type 3UniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
  5. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-636-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:PM0615
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 868868DNA topoisomerase 1UniRule annotation
PRO_0000145160Add
BLAST

Proteomic databases

PRIDEiQ9CN30.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM0615.

Structurei

3D structure databases

ProteinModelPortaliQ9CN30.
SMRiQ9CN30. Positions 3-598, 748-868.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 148146Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 2036Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CN30-1 [UniParc]FASTAAdd to Basket

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MSKSLVIVES PAKAKTINKY LGSNYVVKSS VGHIRDLPTV GSSNGEKAKA    50
ISTKGLSAEE KSAIKQEKDR NALVKRMGID PYHQWKANYQ ILPGKEKVVA 100
ELKSLAKKAD HIYLATDLDR EGEAIAWHLR EVIGGDDARF SRVVFNEITK 150
NAIQQAFEKP EQLNLDRVNA QQTRRFLDRV VGFMVSPLLW KKVARGLSAG 200
RVQSVAVKLV VEREREIKAF QPEEYWEIDV LTQTQAKQAL RLAVTQFKGK 250
KFEPKNATQA QSAVDFLQHA DYVVSDLETK PTHSRAKAPF ITSTLQQTAS 300
TRLGFGVKKT MMLAQRLYEA GYITYMRTDS TNLSQDALKM VRGYIQSHYG 350
ESYLPSKPVF YSSKENAQEA HEAIRPSDVN TLAEQLTGMD KDAVRLYDLI 400
WRQFVACQMP AAQYDSTTVT VRAGEYELKA KGRILRFDGW TKVLPTMSKN 450
AEDQVLPDVQ LNEKLHLEHV LPEQLFTKPP ARFSEAALVK ELEKRGIGRP 500
STYAAIISTI QERGYVRVEN RRFYAEKMGE IVTDRLNQSF TALMNYDFTA 550
NMENILDQIA NGEKNWKTEL NQFFKDFSQQ LSQAELDELE GGMKPNSLVP 600
TDINCPTCGR KMAIRTASTG VFLGCSGYAL PPKERCKTTM NLIPEAELLN 650
VLDEASETKA LMERKRCPKC GTAMDSYLID PERKIHICGH NPNCDGYVLE 700
QGSFKIKGYD GPIVECDKCG ADMHLKLGRF GKYMGCTSCD NTRKILKSGE 750
VAPPKEEPTH FPELKCEKSD AYFVLRDGAS GVFMSAHNFP KSRETRAPKV 800
AELALYRDRL PEKLRYLADA PQHDPEGNDA IVRFSRKEKR QYVTSEKNGK 850
ATKWIVDYLG NQWVERKK 868
Length:868
Mass (Da):98,048
Last modified:June 20, 2001 - v1
Checksum:iE63DF724C4B4ED8D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK02699.1.
RefSeqiNP_245552.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK02699; AAK02699; PM0615.
GeneIDi1243962.
KEGGipmu:PM0615.
PATRICi22870414. VBIPasMul88067_0623.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK02699.1 .
RefSeqi NP_245552.1. NC_002663.1.

3D structure databases

ProteinModelPortali Q9CN30.
SMRi Q9CN30. Positions 3-598, 748-868.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272843.PM0615.

Proteomic databases

PRIDEi Q9CN30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK02699 ; AAK02699 ; PM0615 .
GeneIDi 1243962.
KEGGi pmu:PM0615.
PATRICi 22870414. VBIPasMul88067_0623.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci PMUL272843:GC8W-636-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiTOP1_PASMU
AccessioniPrimary (citable) accession number: Q9CN30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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