Reviewed, UniProtKB/Swiss-Prot Q9CN30 (TOP1_PASMU)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	PM0615

Organism

Pasteurella multocida [Complete proteome] [HAMAP]

Taxonomic identifier

747 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	868 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 868	868	DNA topoisomerase 1		PRO_0000145160
Regions
	Zinc finger	605 – 636	32	C4-type 1
	Zinc finger	667 – 694	28	C4-type 2
	Zinc finger	716 – 739	24	C4-type 3
Sites
	Active site	325	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CN30-1 [UniParc]. Last modified June 20, 2001. Version 1. Checksum: E63DF724C4B4ED8D	FASTA	868	98,048
	10 20 30 40 50 60 MSKSLVIVES PAKAKTINKY LGSNYVVKSS VGHIRDLPTV GSSNGEKAKA ISTKGLSAEE 70 80 90 100 110 120 KSAIKQEKDR NALVKRMGID PYHQWKANYQ ILPGKEKVVA ELKSLAKKAD HIYLATDLDR 130 140 150 160 170 180 EGEAIAWHLR EVIGGDDARF SRVVFNEITK NAIQQAFEKP EQLNLDRVNA QQTRRFLDRV 190 200 210 220 230 240 VGFMVSPLLW KKVARGLSAG RVQSVAVKLV VEREREIKAF QPEEYWEIDV LTQTQAKQAL 250 260 270 280 290 300 RLAVTQFKGK KFEPKNATQA QSAVDFLQHA DYVVSDLETK PTHSRAKAPF ITSTLQQTAS 310 320 330 340 350 360 TRLGFGVKKT MMLAQRLYEA GYITYMRTDS TNLSQDALKM VRGYIQSHYG ESYLPSKPVF 370 380 390 400 410 420 YSSKENAQEA HEAIRPSDVN TLAEQLTGMD KDAVRLYDLI WRQFVACQMP AAQYDSTTVT 430 440 450 460 470 480 VRAGEYELKA KGRILRFDGW TKVLPTMSKN AEDQVLPDVQ LNEKLHLEHV LPEQLFTKPP 490 500 510 520 530 540 ARFSEAALVK ELEKRGIGRP STYAAIISTI QERGYVRVEN RRFYAEKMGE IVTDRLNQSF 550 560 570 580 590 600 TALMNYDFTA NMENILDQIA NGEKNWKTEL NQFFKDFSQQ LSQAELDELE GGMKPNSLVP 610 620 630 640 650 660 TDINCPTCGR KMAIRTASTG VFLGCSGYAL PPKERCKTTM NLIPEAELLN VLDEASETKA 670 680 690 700 710 720 LMERKRCPKC GTAMDSYLID PERKIHICGH NPNCDGYVLE QGSFKIKGYD GPIVECDKCG 730 740 750 760 770 780 ADMHLKLGRF GKYMGCTSCD NTRKILKSGE VAPPKEEPTH FPELKCEKSD AYFVLRDGAS 790 800 810 820 830 840 GVFMSAHNFP KSRETRAPKV AELALYRDRL PEKLRYLADA PQHDPEGNDA IVRFSRKEKR 850 860 QYVTSEKNGK ATKWIVDYLG NQWVERKK

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004439 Genomic DNA. Translation: AAK02699.1.
RefSeq	NP_245552.1.
3D structure databases
HSSP	HSSP built from PDB template 1CY7 based on UniProtKB P06612.
SMR	Q9CN30. Positions 3-598.
ModBase	Search...
Proteomic databases
PRIDE	Q9CN30.
Genome annotation databases
GeneID	1243962.
GenomeReviews	Gene locus PM0615 in contig AE004439_GR.
KEGG	pmu:PM0615.
NMPDR	fig|272843.1.peg.615.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CN30.
OMA	RKFYGCS.
Enzyme and pathway databases
BRENDA	5.99.1.2. 258935.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR013263. TopoI_Znr_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF08272. Topo_Zn_Ribbon. 2 hits. PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_PASMU

Accession

Primary (citable) accession number: Q9CN30

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	June 20, 2001
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)