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Q9CN30

- TOP1_PASMU

UniProt

Q9CN30 - TOP1_PASMU

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
Sitei33 – 331Interaction with DNAUniRule annotation
Metal bindingi117 – 1171Magnesium 1; catalyticUniRule annotation
Metal bindingi117 – 1171Magnesium 2UniRule annotation
Metal bindingi119 – 1191Magnesium 2UniRule annotation
Sitei174 – 1741Interaction with DNAUniRule annotation
Sitei175 – 1751Interaction with DNAUniRule annotation
Sitei178 – 1781Interaction with DNAUniRule annotation
Sitei190 – 1901Interaction with DNAUniRule annotation
Active sitei325 – 3251O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei327 – 3271Interaction with DNAUniRule annotation
Sitei513 – 5131Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri605 – 63632C4-type 1Add
BLAST
Zinc fingeri667 – 69428C4-type 2Add
BLAST
Zinc fingeri716 – 73924C4-type 3Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-636-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:PM0615
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 868868DNA topoisomerase 1PRO_0000145160Add
BLAST

Proteomic databases

PRIDEiQ9CN30.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM0615.

Structurei

3D structure databases

ProteinModelPortaliQ9CN30.
SMRiQ9CN30. Positions 3-598, 748-868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 148146ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 2036Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri605 – 63632C4-type 1Add
BLAST
Zinc fingeri667 – 69428C4-type 2Add
BLAST
Zinc fingeri716 – 73924C4-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CN30-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKSLVIVES PAKAKTINKY LGSNYVVKSS VGHIRDLPTV GSSNGEKAKA
60 70 80 90 100
ISTKGLSAEE KSAIKQEKDR NALVKRMGID PYHQWKANYQ ILPGKEKVVA
110 120 130 140 150
ELKSLAKKAD HIYLATDLDR EGEAIAWHLR EVIGGDDARF SRVVFNEITK
160 170 180 190 200
NAIQQAFEKP EQLNLDRVNA QQTRRFLDRV VGFMVSPLLW KKVARGLSAG
210 220 230 240 250
RVQSVAVKLV VEREREIKAF QPEEYWEIDV LTQTQAKQAL RLAVTQFKGK
260 270 280 290 300
KFEPKNATQA QSAVDFLQHA DYVVSDLETK PTHSRAKAPF ITSTLQQTAS
310 320 330 340 350
TRLGFGVKKT MMLAQRLYEA GYITYMRTDS TNLSQDALKM VRGYIQSHYG
360 370 380 390 400
ESYLPSKPVF YSSKENAQEA HEAIRPSDVN TLAEQLTGMD KDAVRLYDLI
410 420 430 440 450
WRQFVACQMP AAQYDSTTVT VRAGEYELKA KGRILRFDGW TKVLPTMSKN
460 470 480 490 500
AEDQVLPDVQ LNEKLHLEHV LPEQLFTKPP ARFSEAALVK ELEKRGIGRP
510 520 530 540 550
STYAAIISTI QERGYVRVEN RRFYAEKMGE IVTDRLNQSF TALMNYDFTA
560 570 580 590 600
NMENILDQIA NGEKNWKTEL NQFFKDFSQQ LSQAELDELE GGMKPNSLVP
610 620 630 640 650
TDINCPTCGR KMAIRTASTG VFLGCSGYAL PPKERCKTTM NLIPEAELLN
660 670 680 690 700
VLDEASETKA LMERKRCPKC GTAMDSYLID PERKIHICGH NPNCDGYVLE
710 720 730 740 750
QGSFKIKGYD GPIVECDKCG ADMHLKLGRF GKYMGCTSCD NTRKILKSGE
760 770 780 790 800
VAPPKEEPTH FPELKCEKSD AYFVLRDGAS GVFMSAHNFP KSRETRAPKV
810 820 830 840 850
AELALYRDRL PEKLRYLADA PQHDPEGNDA IVRFSRKEKR QYVTSEKNGK
860
ATKWIVDYLG NQWVERKK
Length:868
Mass (Da):98,048
Last modified:June 20, 2001 - v1
Checksum:iE63DF724C4B4ED8D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK02699.1.
RefSeqiNP_245552.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK02699; AAK02699; PM0615.
GeneIDi1243962.
KEGGipmu:PM0615.
PATRICi22870414. VBIPasMul88067_0623.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK02699.1 .
RefSeqi NP_245552.1. NC_002663.1.

3D structure databases

ProteinModelPortali Q9CN30.
SMRi Q9CN30. Positions 3-598, 748-868.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272843.PM0615.

Proteomic databases

PRIDEi Q9CN30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK02699 ; AAK02699 ; PM0615 .
GeneIDi 1243962.
KEGGi pmu:PM0615.
PATRICi 22870414. VBIPasMul88067_0623.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci PMUL272843:GC8W-636-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiTOP1_PASMU
AccessioniPrimary (citable) accession number: Q9CN30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3