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Q9CN30 (TOP1_PASMU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:PM0615
OrganismPasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]
Taxonomic identifier272843 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145160

Regions

Domain3 – 148146Toprim
Zinc finger605 – 63632C4-type 1 HAMAP-Rule MF_00952
Zinc finger667 – 69428C4-type 2 HAMAP-Rule MF_00952
Zinc finger716 – 73924C4-type 3 HAMAP-Rule MF_00952
Region198 – 2036Interaction with DNA By similarity

Sites

Active site3251O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding1171Magnesium 1; catalytic By similarity
Metal binding1171Magnesium 2 By similarity
Metal binding1191Magnesium 2 By similarity
Site331Interaction with DNA By similarity
Site1741Interaction with DNA By similarity
Site1751Interaction with DNA By similarity
Site1781Interaction with DNA By similarity
Site1901Interaction with DNA By similarity
Site3271Interaction with DNA By similarity
Site5131Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CN30 [UniParc].

Last modified June 20, 2001. Version 1.
Checksum: E63DF724C4B4ED8D

FASTA86898,048
        10         20         30         40         50         60 
MSKSLVIVES PAKAKTINKY LGSNYVVKSS VGHIRDLPTV GSSNGEKAKA ISTKGLSAEE 

        70         80         90        100        110        120 
KSAIKQEKDR NALVKRMGID PYHQWKANYQ ILPGKEKVVA ELKSLAKKAD HIYLATDLDR 

       130        140        150        160        170        180 
EGEAIAWHLR EVIGGDDARF SRVVFNEITK NAIQQAFEKP EQLNLDRVNA QQTRRFLDRV 

       190        200        210        220        230        240 
VGFMVSPLLW KKVARGLSAG RVQSVAVKLV VEREREIKAF QPEEYWEIDV LTQTQAKQAL 

       250        260        270        280        290        300 
RLAVTQFKGK KFEPKNATQA QSAVDFLQHA DYVVSDLETK PTHSRAKAPF ITSTLQQTAS 

       310        320        330        340        350        360 
TRLGFGVKKT MMLAQRLYEA GYITYMRTDS TNLSQDALKM VRGYIQSHYG ESYLPSKPVF 

       370        380        390        400        410        420 
YSSKENAQEA HEAIRPSDVN TLAEQLTGMD KDAVRLYDLI WRQFVACQMP AAQYDSTTVT 

       430        440        450        460        470        480 
VRAGEYELKA KGRILRFDGW TKVLPTMSKN AEDQVLPDVQ LNEKLHLEHV LPEQLFTKPP 

       490        500        510        520        530        540 
ARFSEAALVK ELEKRGIGRP STYAAIISTI QERGYVRVEN RRFYAEKMGE IVTDRLNQSF 

       550        560        570        580        590        600 
TALMNYDFTA NMENILDQIA NGEKNWKTEL NQFFKDFSQQ LSQAELDELE GGMKPNSLVP 

       610        620        630        640        650        660 
TDINCPTCGR KMAIRTASTG VFLGCSGYAL PPKERCKTTM NLIPEAELLN VLDEASETKA 

       670        680        690        700        710        720 
LMERKRCPKC GTAMDSYLID PERKIHICGH NPNCDGYVLE QGSFKIKGYD GPIVECDKCG 

       730        740        750        760        770        780 
ADMHLKLGRF GKYMGCTSCD NTRKILKSGE VAPPKEEPTH FPELKCEKSD AYFVLRDGAS 

       790        800        810        820        830        840 
GVFMSAHNFP KSRETRAPKV AELALYRDRL PEKLRYLADA PQHDPEGNDA IVRFSRKEKR 

       850        860 
QYVTSEKNGK ATKWIVDYLG NQWVERKK 

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References

[1]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004439 Genomic DNA. Translation: AAK02699.1.
RefSeqNP_245552.1. NC_002663.1.

3D structure databases

ProteinModelPortalQ9CN30.
SMRQ9CN30. Positions 3-598, 748-868.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272843.PM0615.

Proteomic databases

PRIDEQ9CN30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK02699; AAK02699; PM0615.
GeneID1243962.
KEGGpmu:PM0615.
PATRIC22870414. VBIPasMul88067_0623.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAVVECDKC.
OrthoDBEOG6S7XQ9.

Enzyme and pathway databases

BioCycPMUL272843:GC8W-636-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_PASMU
AccessionPrimary (citable) accession number: Q9CN30
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families