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Q9CM00

- GSHAB_PASMU

UniProt

Q9CM00 - GSHAB_PASMU

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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene
gshAB, gshF, PM1048
Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi702 – 7021Magnesium or manganese 1 By similarity
Metal bindingi723 – 7231Magnesium or manganese 1 By similarity
Metal bindingi723 – 7231Magnesium or manganese 2 By similarity
Metal bindingi725 – 7251Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi521 – 58060ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-1090-MONOMER.
SABIO-RKQ9CM00.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name:
GCS-GS
Including the following 2 domains:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GS
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshAB
Synonyms:gshF
Ordered Locus Names:PM1048
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Glutathione biosynthesis bifunctional protein GshABUniRule annotationPRO_0000192557Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM1048.

Structurei

Secondary structure

1
757
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 106
Helixi13 – 175
Beta strandi19 – 2810
Beta strandi34 – 363
Beta strandi43 – 453
Turni48 – 503
Beta strandi52 – 554
Beta strandi57 – 593
Beta strandi62 – 7211
Helixi73 – 9018
Beta strandi100 – 1023
Helixi120 – 13314
Helixi136 – 1394
Beta strandi144 – 1496
Helixi151 – 16010
Turni162 – 1643
Helixi167 – 19226
Turni204 – 2063
Beta strandi217 – 2193
Beta strandi222 – 2254
Helixi239 – 25113
Beta strandi256 – 2583
Beta strandi264 – 2696
Helixi273 – 2753
Helixi276 – 2794
Beta strandi283 – 2864
Helixi301 – 31616
Helixi323 – 33816
Helixi348 – 36417
Helixi369 – 38315
Helixi385 – 3873
Helixi389 – 39911
Beta strandi400 – 4023
Helixi403 – 41917
Turni420 – 4234
Turni427 – 4304
Helixi433 – 44513
Beta strandi448 – 4536
Turni454 – 4574
Beta strandi458 – 4636
Beta strandi466 – 4716
Turni472 – 4743
Beta strandi477 – 4815
Helixi482 – 4898
Helixi491 – 50010
Beta strandi507 – 5115
Helixi513 – 5186
Helixi519 – 5224
Beta strandi523 – 5264
Beta strandi528 – 5347
Turni537 – 5404
Helixi551 – 56414
Beta strandi566 – 5727
Beta strandi576 – 5849
Beta strandi587 – 5948
Beta strandi597 – 6004
Helixi601 – 6033
Helixi607 – 6159
Beta strandi623 – 6297
Helixi635 – 64410
Beta strandi648 – 6503
Beta strandi657 – 6593
Helixi666 – 6683
Beta strandi672 – 6743
Turni676 – 6783
Helixi681 – 69414
Beta strandi697 – 70610
Beta strandi708 – 7103
Turni716 – 7183
Beta strandi720 – 7278
Helixi730 – 7345
Beta strandi737 – 7393
Helixi745 – 7528

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LN7X-ray3.20A/B1-757[»]
ProteinModelPortaliQ9CM00.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini494 – 753260ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 337337Glutamate--cysteine ligaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiHVEYVKN.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CM00-1 [UniParc]FASTAAdd to Basket

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MKIQHIIHEN QLGLLFQQGS FGLEKESQRV TADGAIVTTP HPAVFGNRRY    50
HPYIQTDFAE SQLELITPPT KKLEDTFRWL SVIHEVVQRS LPEEEYIFPL 100
SMPAGLPAEE QIRVAQLDNP EDVAYREYLV KIYGKNKQMV SGIHYNFQLS 150
PDLITRLFRL QNEYQSAVDF QNDLYLKMAK NFLRYQWILL YLLAATPTVE 200
SAYFKDGSPL AKGQFVRSLR SSQYGYVNDP EINVSFDSVE KYVESLEHWV 250
STGKLIAEKE FYSNVRLRGA KKAREFLTTG IQYLEFRLFD LNPFEIYGIS 300
LKDAKFIHVF ALFMIWMDHT ADQEEVELGK ARLAEVAFEH PLEKTAYAVE 350
GELVLLELLS MLEQIGAEPE LFEIVKEKLT QFTDPSKTVA GRLVRAIEQA 400
GSDQQLGAQL AQQYKAQAFE RFYALSAFDN MELSTQALLF DVIQKGIHTE 450
ILDENDQFLC LKYGDHIEYV KNGNMTSHDS YISPLIMENK VVTKKVLQKA 500
GFNVPQSVEF TSLEKAVASY ALFENRAVVI KPKSTNYGLG ITIFQQGVQN 550
REDFAKALEI AFREDKEVMV EDYLVGTEYR FFVLGDETLA VLLRVPANVV 600
GDSVHSVAEL VAMKNDHPLR GDGSRTPLKK IALGEIEQLQ LKEQGLTIDS 650
IPAKDQLVQL RANSNISTGG DSIDMTDEMH ESYKQLAVGI TKAMGAAVCG 700
VDLIIPDLKQ PATPNLTSWG VIEANFNPMM MMHIFPYAGK SRRLTQNVIK 750
MLFPELE 757
Length:757
Mass (Da):85,862
Last modified:June 1, 2001 - v1
Checksum:iCF0E49A661C14336
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK03132.1.
RefSeqiNP_245985.1. NC_002663.1.
WP_010906990.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK03132; AAK03132; PM1048.
GeneIDi1244395.
KEGGipmu:PM1048.
PATRICi22871339. VBIPasMul88067_1062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK03132.1 .
RefSeqi NP_245985.1. NC_002663.1.
WP_010906990.1. NC_002663.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LN7 X-ray 3.20 A/B 1-757 [» ]
ProteinModelPortali Q9CM00.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272843.PM1048.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK03132 ; AAK03132 ; PM1048 .
GeneIDi 1244395.
KEGGi pmu:PM1048.
PATRICi 22871339. VBIPasMul88067_1062.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OMAi HVEYVKN.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci PMUL272843:GC8W-1090-MONOMER.
SABIO-RK Q9CM00.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 3 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiGSHAB_PASMU
AccessioniPrimary (citable) accession number: Q9CM00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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