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Q9CM00

- GSHAB_PASMU

UniProt

Q9CM00 - GSHAB_PASMU

Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi702 – 7021Magnesium or manganese 1UniRule annotation
    Metal bindingi723 – 7231Magnesium or manganese 1UniRule annotation
    Metal bindingi723 – 7231Magnesium or manganese 2UniRule annotation
    Metal bindingi725 – 7251Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi521 – 58060ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
    3. glutathione synthase activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPMUL272843:GC8W-1090-MONOMER.
    SABIO-RKQ9CM00.
    UniPathwayiUPA00142; UER00209.
    UPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione biosynthesis bifunctional protein GshABUniRule annotation
    Alternative name(s):
    Gamma-GCS-GSUniRule annotation
    Short name:
    GCS-GSUniRule annotation
    Including the following 2 domains:
    Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
    Alternative name(s):
    Gamma-ECSUniRule annotation
    Short name:
    GCSUniRule annotation
    Gamma-glutamylcysteine synthetaseUniRule annotation
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshABUniRule annotation
    Synonyms:gshFUniRule annotation
    Ordered Locus Names:PM1048
    OrganismiPasteurella multocida (strain Pm70)
    Taxonomic identifieri272843 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
    ProteomesiUP000000809: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757Glutathione biosynthesis bifunctional protein GshABPRO_0000192557Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272843.PM1048.

    Structurei

    Secondary structure

    1
    757
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 106
    Helixi13 – 175
    Beta strandi19 – 2810
    Beta strandi34 – 363
    Beta strandi43 – 453
    Turni48 – 503
    Beta strandi52 – 554
    Beta strandi57 – 593
    Beta strandi62 – 7211
    Helixi73 – 9018
    Beta strandi100 – 1023
    Helixi120 – 13314
    Helixi136 – 1394
    Beta strandi144 – 1496
    Helixi151 – 16010
    Turni162 – 1643
    Helixi167 – 19226
    Turni204 – 2063
    Beta strandi217 – 2193
    Beta strandi222 – 2254
    Helixi239 – 25113
    Beta strandi256 – 2583
    Beta strandi264 – 2696
    Helixi273 – 2753
    Helixi276 – 2794
    Beta strandi283 – 2864
    Helixi301 – 31616
    Helixi323 – 33816
    Helixi348 – 36417
    Helixi369 – 38315
    Helixi385 – 3873
    Helixi389 – 39911
    Beta strandi400 – 4023
    Helixi403 – 41917
    Turni420 – 4234
    Turni427 – 4304
    Helixi433 – 44513
    Beta strandi448 – 4536
    Turni454 – 4574
    Beta strandi458 – 4636
    Beta strandi466 – 4716
    Turni472 – 4743
    Beta strandi477 – 4815
    Helixi482 – 4898
    Helixi491 – 50010
    Beta strandi507 – 5115
    Helixi513 – 5186
    Helixi519 – 5224
    Beta strandi523 – 5264
    Beta strandi528 – 5347
    Turni537 – 5404
    Helixi551 – 56414
    Beta strandi566 – 5727
    Beta strandi576 – 5849
    Beta strandi587 – 5948
    Beta strandi597 – 6004
    Helixi601 – 6033
    Helixi607 – 6159
    Beta strandi623 – 6297
    Helixi635 – 64410
    Beta strandi648 – 6503
    Beta strandi657 – 6593
    Helixi666 – 6683
    Beta strandi672 – 6743
    Turni676 – 6783
    Helixi681 – 69414
    Beta strandi697 – 70610
    Beta strandi708 – 7103
    Turni716 – 7183
    Beta strandi720 – 7278
    Helixi730 – 7345
    Beta strandi737 – 7393
    Helixi745 – 7528

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LN7X-ray3.20A/B1-757[»]
    ProteinModelPortaliQ9CM00.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini494 – 753260ATP-graspUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 337337Glutamate--cysteine ligaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1181.
    HOGENOMiHOG000156471.
    KOiK01919.
    OMAiHVEYVKN.
    OrthoDBiEOG6BKJ7H.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 3 hits.
    HAMAPiMF_00782. Glut_biosynth.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view]
    PfamiPF04262. Glu_cys_ligase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CM00-1 [UniParc]FASTAAdd to Basket

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    MKIQHIIHEN QLGLLFQQGS FGLEKESQRV TADGAIVTTP HPAVFGNRRY    50
    HPYIQTDFAE SQLELITPPT KKLEDTFRWL SVIHEVVQRS LPEEEYIFPL 100
    SMPAGLPAEE QIRVAQLDNP EDVAYREYLV KIYGKNKQMV SGIHYNFQLS 150
    PDLITRLFRL QNEYQSAVDF QNDLYLKMAK NFLRYQWILL YLLAATPTVE 200
    SAYFKDGSPL AKGQFVRSLR SSQYGYVNDP EINVSFDSVE KYVESLEHWV 250
    STGKLIAEKE FYSNVRLRGA KKAREFLTTG IQYLEFRLFD LNPFEIYGIS 300
    LKDAKFIHVF ALFMIWMDHT ADQEEVELGK ARLAEVAFEH PLEKTAYAVE 350
    GELVLLELLS MLEQIGAEPE LFEIVKEKLT QFTDPSKTVA GRLVRAIEQA 400
    GSDQQLGAQL AQQYKAQAFE RFYALSAFDN MELSTQALLF DVIQKGIHTE 450
    ILDENDQFLC LKYGDHIEYV KNGNMTSHDS YISPLIMENK VVTKKVLQKA 500
    GFNVPQSVEF TSLEKAVASY ALFENRAVVI KPKSTNYGLG ITIFQQGVQN 550
    REDFAKALEI AFREDKEVMV EDYLVGTEYR FFVLGDETLA VLLRVPANVV 600
    GDSVHSVAEL VAMKNDHPLR GDGSRTPLKK IALGEIEQLQ LKEQGLTIDS 650
    IPAKDQLVQL RANSNISTGG DSIDMTDEMH ESYKQLAVGI TKAMGAAVCG 700
    VDLIIPDLKQ PATPNLTSWG VIEANFNPMM MMHIFPYAGK SRRLTQNVIK 750
    MLFPELE 757
    Length:757
    Mass (Da):85,862
    Last modified:June 1, 2001 - v1
    Checksum:iCF0E49A661C14336
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004439 Genomic DNA. Translation: AAK03132.1.
    RefSeqiNP_245985.1. NC_002663.1.
    WP_010906990.1. NC_002663.1.

    Genome annotation databases

    EnsemblBacteriaiAAK03132; AAK03132; PM1048.
    GeneIDi1244395.
    KEGGipmu:PM1048.
    PATRICi22871339. VBIPasMul88067_1062.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004439 Genomic DNA. Translation: AAK03132.1 .
    RefSeqi NP_245985.1. NC_002663.1.
    WP_010906990.1. NC_002663.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LN7 X-ray 3.20 A/B 1-757 [» ]
    ProteinModelPortali Q9CM00.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272843.PM1048.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK03132 ; AAK03132 ; PM1048 .
    GeneIDi 1244395.
    KEGGi pmu:PM1048.
    PATRICi 22871339. VBIPasMul88067_1062.

    Phylogenomic databases

    eggNOGi COG1181.
    HOGENOMi HOG000156471.
    KOi K01919.
    OMAi HVEYVKN.
    OrthoDBi EOG6BKJ7H.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00209 .
    UPA00142 ; UER00210 .
    BioCyci PMUL272843:GC8W-1090-MONOMER.
    SABIO-RK Q9CM00.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 3 hits.
    HAMAPi MF_00782. Glut_biosynth.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view ]
    Pfami PF04262. Glu_cys_ligase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pm70.

    Entry informationi

    Entry nameiGSHAB_PASMU
    AccessioniPrimary (citable) accession number: Q9CM00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3