Reviewed, UniProtKB/Swiss-Prot Q9CLY8 (PTM3C_PASMU)

Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific EIICBA component
Alternative name(s):
    EIICBA-Mtl
      Short name=EII-Mtl
Including the following 3 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component
    3- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system mannitol-specific EIIA component

Gene names

Name:	mtlA
Ordered Locus Names:	PM1061

Organism

Pasteurella multocida [Complete proteome] [HAMAP]

Taxonomic identifier

747 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Post-translational modification	An intramolecular phosphotransfer takes places between His-542 and Cys-378 By similarity.
Sequence similarities	Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	PTS system mannitol-specific EIICBA component		PRO_0000186616
Regions
	Transmembrane	25 – 45	21	Potential
	Transmembrane	53 – 73	21	Potential
	Transmembrane	79 – 99	21	Potential
	Transmembrane	134 – 154	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	270 – 290	21	Potential
	Transmembrane	313 – 333	21	Potential
	Domain	13 – 336	324	PTS EIIC type-2
	Domain	372 – 463	92	PTS EIIB type-2
	Domain	482 – 624	143	PTS EIIA type-2
Sites
	Active site	378	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	542	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CLY8-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EA104312CEE2D891	FASTA	624	66,550
	10 20 30 40 50 60 MLSANAKVKV QNFGRFLSNM VMPNIGAFIA WGFITALFIP TGWLPNETLA KLVGPMITYL 70 80 90 100 110 120 LPLLIGYSGG KLIAGERGAV VGAIATAGVI VGTDIPMFLG AMIAGPTGGW AIKRFDKWAD 130 140 150 160 170 180 GKIKSGFEML VNNFSSGIIG MILAILFFWL IGPAVKALST MLAAGVDILV KAHLLPLTSI 190 200 210 220 230 240 FVEPAKILFL NNAINHGIFS PLGIQQSQEF GQSIFFLIEA NPGPGLGVLL AYIIFGKGTA 250 260 270 280 290 300 KQTAGGATII HFFGGIHEIY FPYVLMNPRL LLAVIAGGVS GVFTLVLFNA GLVAPASPGS 310 320 330 340 350 360 IIAVLLMTPQ NAIVGVLASV AIAATVSFVI ASFFLKIQKE ENGHSLEKMQ AASKAMKSGV 370 380 390 400 410 420 QFNTPARYQG VQKIFVACDA GMGSSAMGAS MLRKKVKEAG LAIEVTNCAI NDLPEDAQLV 430 440 450 460 470 480 ITHQDLTLRA KKHTPNAMHF SLNNFLDAHF YDNLVQDLSN TKVADLAKVS TLEPQEAPQT 490 500 510 520 530 540 AFVLTEKQVF LGLKAANKEE AIRFAGERLV ESGFVLPSYV DAMFEREKMV STYLGEGIAV 550 560 570 580 590 600 PHGTIEAKDA VLKTGVVVCQ YPEGVKFNED EEDSIAKLVI GIAAKNNEHL QVVSAITNAL 610 620 DNEDAIRILS ETDDVEKVLA LLKA

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK03145.1. Different initiation.
RefSeq	NP_245998.1.
3D structure databases
SMR	Q9CLY8. Positions 371-458, 482-622.
ModBase	Search...
Genome annotation databases
GeneID	1244408.
GenomeReviews	Gene locus PM1061 in contig AE004439_GR.
KEGG	pmu:PM1061.
NMPDR	fig|272843.1.peg.1061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296680.
OMA	GMIMPNI.
Enzyme and pathway databases
BRENDA	2.7.1.69. 258935.
Family and domain databases
InterPro	IPR016152. PTrfase/Anion_transptr. IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR004718. PTS_IIC_mtl. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
Pfam	PF00359. PTS_EIIA_2. 1 hit. PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00851. mtlA. 1 hit.
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTM3C_PASMU

Accession

Primary (citable) accession number: Q9CLY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)