Reviewed, UniProtKB/Swiss-Prot Q9CLW4 (PUR4_PASMU)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	PM1085

Organism

Pasteurella multocida [Complete proteome] [HAMAP]

Taxonomic identifier

747 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	1297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1297	1297	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100412
Regions
	Domain	1043 – 1297	255	Glutamine amidotransferase type-1
	Nucleotide binding	307 – 318	12	ATP Potential
Sites
	Active site	1137	1	Nucleophile By similarity
	Active site	1262	1	By similarity
	Active site	1264	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CLW4-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 9362706FEFE34CB4	FASTA	1,297	143,043
	10 20 30 40 50 60 MLQIFRGSPA LSEFRLNQLA VRFQKANLPV SACYAEYIHF ADLSDRLTEE ETAKLDQLLH 70 80 90 100 110 120 YGPTLAGHDP VGHCFIVIPR IGTISSWSSK ATDIAHNCGL NKVNRLERGL AFYFEFDRTL 130 140 150 160 170 180 SSEEQQRLVS HIHDRMLEGI IHTPEEAKVL FDQQAPKPFT TVDILSGGRQ ALENANVELG 190 200 210 220 230 240 LALAEDEIDY LVENFTALGR NPNDIELYMF AQANSEHCRH KIFNADWIID GEKQEKSLFK 250 260 270 280 290 300 MIKNTFEKTP DHVLSAYKDN AAVMEGSKVG RFFPDQDGQY RYHNEDAHIL MKVETHNHPT 310 320 330 340 350 360 AISPFPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL VIPGFEQPWE NPVSKPNRIA 370 380 390 400 410 420 SALDIMIEGP LGGAAFNNEF GRPALLGYFR TYEEKVNSFA GEEVRGYHKP IMLAGGIGNI 430 440 450 460 470 480 RAEHVQKGEI PVGAKLIVLG GPAMNIGLGG GAASSMASGK SKEDLDFASV QRDNPEMERR 490 500 510 520 530 540 CQEVIDRCWQ LGEDNPILFI HDVGAGGLSN AMPELVHDGG RGGKFELRKI LSDERGMSPL 550 560 570 580 590 600 EIWCNESQER YVLAVAPEKL ELFTALCERE RAPFAVIGEA TEQEHLTLHD DHFDNNPIDL 610 620 630 640 650 660 PMNVLLGKTP KMTRDVKSKT VEGSALDHSQ IDLKEAFHRV LRLPVVAEKT FLITIGDRSV 670 680 690 700 710 720 TGMVARDQMV GPWQIPVADC AVTTASLDSY HGEAMSMGER APVALLDFAA SARLAVAESI 730 740 750 760 770 780 TNIAATNIGD IKRIKLSANW MSAAGHEGED AGLYQAVKAV GEELCPQLGL TIPVGKDSMS 790 800 810 820 830 840 MKTTWHENGE QKTVTAPLSL VISAFARVED VRKTVTPQLR TDKGHSRLLL IDLGEGKNRL 850 860 870 880 890 900 GATALAQVYK QLGDKPADVV NVESLKNFFN AMQALVAEQK LLAYHDRSDG GLIATLAEMA 910 920 930 940 950 960 FAGNCGLSIH ISALGDNDLA VLFNEELGAV IQVRESDLSY VRDVLSQHGL IHLTKELGEV 970 980 990 1000 1010 1020 TTEDRIEISR GTKLLFSQKR SELRGIWAEL THQMQRLRDN PECADQEFAA KKDPENKGFS 1030 1040 1050 1060 1070 1080 AHLTYDINED IAAPYIATGK KPRIAILREQ GVNSHYEMAA AFDRAGFEAI DVHMSDLHNA 1090 1100 1110 1120 1130 1140 RYRLKDFNAL VACGGFSYGD VLGAGGGWAK SILFNPMLRD QFSEFFANPN TLTLGVCNGC 1150 1160 1170 1180 1190 1200 QMVSNLAEII PGTDAWPRFV RNKSERFEAR AALVRINETN SLWFQGMAGS HMPIAVSHGE 1210 1220 1230 1240 1250 1260 GRVEFKHDQQ LQMLKDQNLI VAQYIDNNLN PTEIYPANPN GSVEGITALS NQDGRVAIMM 1270 1280 1290 PHPERVFRTV SNSWHPEDWS EDGAWMRLFR NARVVLE

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004439 Genomic DNA. Translation: AAK03169.1.
RefSeq	NP_246022.1.
3D structure databases
SMR	Q9CLW4. Positions 1-1293.
ModBase	Search...
Genome annotation databases
GeneID	1244432.
GenomeReviews	Gene locus PM1085 in contig AE004439_GR.
KEGG	pmu:PM1085.
NMPDR	fig|272843.1.peg.1085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9CLW4.
OMA	ERGIAYY.
Enzyme and pathway databases
BRENDA	6.3.5.3. 258935.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_PASMU

Accession

Primary (citable) accession number: Q9CLW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)