Q9CLN7 (PLSB_PASMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PM1182

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 809	809	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_0000195226
Regions
	Motif	306 – 311	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q9CLN7 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 63CFE636A090382D	FASTA	809	92,674
	10 20 30 40 50 60 MFSLLNMYRK VLELPLSFLV KNNPIPHHPI EELKLDPTQP MVYVLPYTSQ TDLVIFRKNC 70 80 90 100 110 120 LSVGLPDPLE KNEIEGKSLP RFVFLDEGRR FFKSKGAKEE TIQVFNQYLE LHHQRSDLDV 130 140 150 160 170 180 QIIPVSVLWG RSPGHEKQSG LPQLRLLSGL QKFIAAVWFG RDTFVRFSQA VSLRYMVNEH 190 200 210 220 230 240 GSDQAISHKL ARVAKIHFSK QRISATGPRL PNREAMFNKL LASPTILDAI QDEAKAKNIS 250 260 270 280 290 300 EEKATQEAKK ILDEIAANVN YEGLRVADRF LRWLWNKLYQ GIDVQNADRV RKLALDGHEI 310 320 330 340 350 360 VYVPCHRSHI DYLLLSYVLY HQGLVPPHIA AGINLNFWPV GGMFRRGGAF FIRRTFKGNR 370 380 390 400 410 420 LYSTIFREYL AELFHRGYSV EYFIEGGRSR TGRLLAPKTG MMSMTLQALL QGQTRPISVV 430 440 450 460 470 480 PVYVGYEHVL EVDTYAKELR GAAKEKENAG LVLRVIKKLR NLGKGYVNFG EPITLSNYLN 490 500 510 520 530 540 QHYPEWKENT QAEERAKWFN QAVDSISHQV MVNINKAAAV NAMNLTGTAL LSSRQRALSR 550 560 570 580 590 600 EQLLEQLESY QQFLQNVPYS QDIVVPTESP EKMLEHILQL ERVGIIVEKD NFGEIVRLER 610 620 630 640 650 660 NSAVLMTYYR NNIQHLFVLP SLVASIVLHY EAIQKPLVLE AVSKIYPFLK SELFLSFSYE 670 680 690 700 710 720 ELQDRVNHII TEFERQEVIK CNANMLSINK PRVRMLQLWS SGVREILQRY YITVSILEKD 730 740 750 760 770 780 PATPRASLEK DSQSVAQRLS VLHGINAPEF FDKAVFSAFI ASLKQEGYFN EEGIANTEKL 790 800 NEITEILNQV ISTEVCLTIK GAIAKIEEE

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK03266.1.
RefSeq	NP_246119.1. NC_002663.1.
3D structure databases
ProteinModelPortal	Q9CLN7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1244529.
GenomeReviews	Gene locus PM1182 in contig AE004439_GR.
KEGG	pmu:PM1182.
NMPDR	fig|272843.1.peg.1182.
PATRIC	22871617. VBIPasMul88067_1193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	PMUL272843:PM1182-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_PASMU

Accession

Primary (citable) accession number: Q9CLN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program