Q9CLC2 (TRMB_PASMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	PM1317

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella › Pasteurella multocida › Pasteurella multocida subsp. multocida

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171368
Regions
	Region	234 – 237	4	Substrate binding Potential
Sites
	Binding site	86	1	S-adenosyl-L-methionine By similarity
	Binding site	111	1	S-adenosyl-L-methionine By similarity
	Binding site	138	1	S-adenosyl-L-methionine By similarity
	Binding site	161	1	S-adenosyl-L-methionine By similarity
	Binding site	165	1	Substrate By similarity
	Binding site	197	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9CLC2 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D3F07ADF145D2980	FASTA	255	29,319
	10 20 30 40 50 60 MSFNNQESEN LPTFADQKRK TVEVAEFTAE GRYKRKVRSF VLRTGRLSEF QKNAMNQHWG 70 80 90 100 110 120 FYGLEHQNQP FDFPAIYGNN NPVVLEIGFG MGHSLVDMAL QNPERNYLGI EVHTPGVGAC 130 140 150 160 170 180 IAYAVEKGVK NLRIICHDAT EILRDCIADH SLGGLQLFFP DPWHKAKHHK RRIVQPQFVN 190 200 210 220 230 240 TITQKLTTGG FIHMATDWEN YAEHMLAVLQ QAEGLQNTSA THDYIPRPDF RPLTKFEQRG 250 HRLGHGVWDL YYIKK

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK03401.1.
RefSeq	NP_246255.1. NC_002663.1.
3D structure databases
ProteinModelPortal	Q9CLC2.
ModBase	Search...
Protein-protein interaction databases
STRING	272843.PM1317.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK03401; AAK03401; PM1317.
GeneID	1244664.
KEGG	pmu:PM1317.
PATRIC	22871887. VBIPasMul88067_1328.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000073968.
KO	K03439.
OMA	CEVHEPG.
ProtClustDB	PRK00121.
Enzyme and pathway databases
BioCyc	PMUL272843:GC8W-1368-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_PASMU

Accession

Primary (citable) accession number: Q9CLC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	May 29, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program