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Q9CLC2 (TRMB_PASMU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:PM1317
OrganismPasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]
Taxonomic identifier272843 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171368

Regions

Region234 – 2374Substrate binding By similarity

Sites

Active site1611 By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1111S-adenosyl-L-methionine By similarity
Binding site1381S-adenosyl-L-methionine By similarity
Binding site1611S-adenosyl-L-methionine By similarity
Binding site1651Substrate By similarity
Binding site1971Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CLC2 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D3F07ADF145D2980

FASTA25529,319
        10         20         30         40         50         60 
MSFNNQESEN LPTFADQKRK TVEVAEFTAE GRYKRKVRSF VLRTGRLSEF QKNAMNQHWG 

        70         80         90        100        110        120 
FYGLEHQNQP FDFPAIYGNN NPVVLEIGFG MGHSLVDMAL QNPERNYLGI EVHTPGVGAC 

       130        140        150        160        170        180 
IAYAVEKGVK NLRIICHDAT EILRDCIADH SLGGLQLFFP DPWHKAKHHK RRIVQPQFVN 

       190        200        210        220        230        240 
TITQKLTTGG FIHMATDWEN YAEHMLAVLQ QAEGLQNTSA THDYIPRPDF RPLTKFEQRG 

       250 
HRLGHGVWDL YYIKK 

« Hide

References

[1]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004439 Genomic DNA. Translation: AAK03401.1.
RefSeqNP_246255.1. NC_002663.1.

3D structure databases

ProteinModelPortalQ9CLC2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272843.PM1317.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK03401; AAK03401; PM1317.
GeneID1244664.
KEGGpmu:PM1317.
PATRIC22871887. VBIPasMul88067_1328.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMADNYWPVM.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycPMUL272843:GC8W-1368-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PASMU
AccessionPrimary (citable) accession number: Q9CLC2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways