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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi52 – 521Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi80 – 801Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-1656-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:PM1594
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 828796Periplasmic nitrate reductasePRO_0000045992Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PRIDEiQ9CKL8.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM1594.

Structurei

3D structure databases

ProteinModelPortaliQ9CKL8.
SMRiQ9CKL8. Positions 38-827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 94574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiFTTDEMW.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CKL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSRRDFMK TNAAVAAAAV AGLAIPVKNV EASPDKIIKW DKAPCRFCGT
60 70 80 90 100
GCSVLVGTQN GRVVASQGDP DADVNRGLNC IKGYFLPKIM YGKDRLTSPM
110 120 130 140 150
LRMTNGKFDK HGEFTPVSWD QAFTIMAEKF KKALKEKGPN GAGMFTSGQS
160 170 180 190 200
TIFEGIAKSK LFKAGLRSNN IDPNARHCMA SAAVAFMRTF GMDEPMGCYD
210 220 230 240 250
DIEKADAFVL WGSNMAEMHP ILWSRISDRR LSHPDVKVAV LSTFEHRSFE
260 270 280 290 300
LADLGVIFTP QSDLAIMNYI ANYLIQHDAI DHDFIQKHTK FKRGETDIGY
310 320 330 340 350
GLRETHELEK AAKNVKTAGK MHDSDFEEYK KLVAPYTLEK AHEISGVPKE
360 370 380 390 400
QLEALAKMYA DPKLNIVSFW TMGFNQHTRG VWANHLIYNI HLLTGKISKP
410 420 430 440 450
GCGPFSLTGQ PSACGTAREV GTFAHRLPAD LVVTNPKHVE KAEKLWKLPK
460 470 480 490 500
GVIQTQVGYH AVAQDRALKD KKMNVLWQMC TNNMQGGPNI NQERFPGWRD
510 520 530 540 550
EENFVIVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER RTQFWYQQVK
560 570 580 590 600
APGESKSDVW QLVEFSKYFT TDEMWPAEVL AANPEYKGKT LYEVLFRNGQ
610 620 630 640 650
VDKFQVPTDK PGYMNDEAEH FGFYLQKGLF EEYAVFGRGH GHDLADFETY
660 670 680 690 700
HKARGLRWPV VDGKETLWRY REGYDPYVKE GEGVAFYGYP DKKAIILAVP
710 720 730 740 750
YEPPAEAPNA EYDLWLTTGR VLEHWHTGTM TRRVPELHRS FPNNLVWMHP
760 770 780 790 800
LDAKKRGLRH GDKVKISSRR GEMISHLDTR GRNKVPQGLV YTTFFDAGQL
810 820
ANYLTLDATD PISKETDFKK CAVKVEKA
Length:828
Mass (Da):93,356
Last modified:June 1, 2001 - v1
Checksum:i313637544961A2BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03678.1.
RefSeqiNP_246533.1. NC_002663.1.
WP_005724436.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK03678; AAK03678; PM1594.
GeneIDi1244941.
KEGGipmu:PM1594.
PATRICi22872479. VBIPasMul88067_1613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03678.1.
RefSeqiNP_246533.1. NC_002663.1.
WP_005724436.1. NC_002663.1.

3D structure databases

ProteinModelPortaliQ9CKL8.
SMRiQ9CKL8. Positions 38-827.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272843.PM1594.

Proteomic databases

PRIDEiQ9CKL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK03678; AAK03678; PM1594.
GeneIDi1244941.
KEGGipmu:PM1594.
PATRICi22872479. VBIPasMul88067_1613.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiFTTDEMW.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-1656-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiNAPA_PASMU
AccessioniPrimary (citable) accession number: Q9CKL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 2001
Last modified: May 27, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.