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Q9CKL8 (NAPA_PASMU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:PM1594
OrganismPasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]
Taxonomic identifier272843 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 828796Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045992

Regions

Domain38 – 94574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding451Iron-sulfur (4Fe-4S) By similarity
Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding521Iron-sulfur (4Fe-4S) By similarity
Metal binding801Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CKL8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 313637544961A2BF

FASTA82893,356
        10         20         30         40         50         60 
MNLSRRDFMK TNAAVAAAAV AGLAIPVKNV EASPDKIIKW DKAPCRFCGT GCSVLVGTQN 

        70         80         90        100        110        120 
GRVVASQGDP DADVNRGLNC IKGYFLPKIM YGKDRLTSPM LRMTNGKFDK HGEFTPVSWD 

       130        140        150        160        170        180 
QAFTIMAEKF KKALKEKGPN GAGMFTSGQS TIFEGIAKSK LFKAGLRSNN IDPNARHCMA 

       190        200        210        220        230        240 
SAAVAFMRTF GMDEPMGCYD DIEKADAFVL WGSNMAEMHP ILWSRISDRR LSHPDVKVAV 

       250        260        270        280        290        300 
LSTFEHRSFE LADLGVIFTP QSDLAIMNYI ANYLIQHDAI DHDFIQKHTK FKRGETDIGY 

       310        320        330        340        350        360 
GLRETHELEK AAKNVKTAGK MHDSDFEEYK KLVAPYTLEK AHEISGVPKE QLEALAKMYA 

       370        380        390        400        410        420 
DPKLNIVSFW TMGFNQHTRG VWANHLIYNI HLLTGKISKP GCGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFAHRLPAD LVVTNPKHVE KAEKLWKLPK GVIQTQVGYH AVAQDRALKD KKMNVLWQMC 

       490        500        510        520        530        540 
TNNMQGGPNI NQERFPGWRD EENFVIVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWYQQVK APGESKSDVW QLVEFSKYFT TDEMWPAEVL AANPEYKGKT LYEVLFRNGQ 

       610        620        630        640        650        660 
VDKFQVPTDK PGYMNDEAEH FGFYLQKGLF EEYAVFGRGH GHDLADFETY HKARGLRWPV 

       670        680        690        700        710        720 
VDGKETLWRY REGYDPYVKE GEGVAFYGYP DKKAIILAVP YEPPAEAPNA EYDLWLTTGR 

       730        740        750        760        770        780 
VLEHWHTGTM TRRVPELHRS FPNNLVWMHP LDAKKRGLRH GDKVKISSRR GEMISHLDTR 

       790        800        810        820 
GRNKVPQGLV YTTFFDAGQL ANYLTLDATD PISKETDFKK CAVKVEKA 

« Hide

References

[1]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004439 Genomic DNA. Translation: AAK03678.1.
RefSeqNP_246533.1. NC_002663.1.

3D structure databases

ProteinModelPortalQ9CKL8.
SMRQ9CKL8. Positions 38-827.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272843.PM1594.

Proteomic databases

PRIDEQ9CKL8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK03678; AAK03678; PM1594.
GeneID1244941.
KEGGpmu:PM1594.
PATRIC22872479. VBIPasMul88067_1613.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFTTDEMW.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycPMUL272843:GC8W-1656-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_PASMU
AccessionPrimary (citable) accession number: Q9CKL8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families