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Q9CKL8

- NAPA_PASMU

UniProt

Q9CKL8 - NAPA_PASMU

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi52 – 521Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi80 – 801Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-1656-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:PM1594
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 828796Periplasmic nitrate reductasePRO_0000045992Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PRIDEiQ9CKL8.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM1594.

Structurei

3D structure databases

ProteinModelPortaliQ9CKL8.
SMRiQ9CKL8. Positions 38-827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 94574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiAGKMHDS.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CKL8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNLSRRDFMK TNAAVAAAAV AGLAIPVKNV EASPDKIIKW DKAPCRFCGT
60 70 80 90 100
GCSVLVGTQN GRVVASQGDP DADVNRGLNC IKGYFLPKIM YGKDRLTSPM
110 120 130 140 150
LRMTNGKFDK HGEFTPVSWD QAFTIMAEKF KKALKEKGPN GAGMFTSGQS
160 170 180 190 200
TIFEGIAKSK LFKAGLRSNN IDPNARHCMA SAAVAFMRTF GMDEPMGCYD
210 220 230 240 250
DIEKADAFVL WGSNMAEMHP ILWSRISDRR LSHPDVKVAV LSTFEHRSFE
260 270 280 290 300
LADLGVIFTP QSDLAIMNYI ANYLIQHDAI DHDFIQKHTK FKRGETDIGY
310 320 330 340 350
GLRETHELEK AAKNVKTAGK MHDSDFEEYK KLVAPYTLEK AHEISGVPKE
360 370 380 390 400
QLEALAKMYA DPKLNIVSFW TMGFNQHTRG VWANHLIYNI HLLTGKISKP
410 420 430 440 450
GCGPFSLTGQ PSACGTAREV GTFAHRLPAD LVVTNPKHVE KAEKLWKLPK
460 470 480 490 500
GVIQTQVGYH AVAQDRALKD KKMNVLWQMC TNNMQGGPNI NQERFPGWRD
510 520 530 540 550
EENFVIVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER RTQFWYQQVK
560 570 580 590 600
APGESKSDVW QLVEFSKYFT TDEMWPAEVL AANPEYKGKT LYEVLFRNGQ
610 620 630 640 650
VDKFQVPTDK PGYMNDEAEH FGFYLQKGLF EEYAVFGRGH GHDLADFETY
660 670 680 690 700
HKARGLRWPV VDGKETLWRY REGYDPYVKE GEGVAFYGYP DKKAIILAVP
710 720 730 740 750
YEPPAEAPNA EYDLWLTTGR VLEHWHTGTM TRRVPELHRS FPNNLVWMHP
760 770 780 790 800
LDAKKRGLRH GDKVKISSRR GEMISHLDTR GRNKVPQGLV YTTFFDAGQL
810 820
ANYLTLDATD PISKETDFKK CAVKVEKA
Length:828
Mass (Da):93,356
Last modified:June 1, 2001 - v1
Checksum:i313637544961A2BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03678.1.
RefSeqiNP_246533.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK03678; AAK03678; PM1594.
GeneIDi1244941.
KEGGipmu:PM1594.
PATRICi22872479. VBIPasMul88067_1613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03678.1 .
RefSeqi NP_246533.1. NC_002663.1.

3D structure databases

ProteinModelPortali Q9CKL8.
SMRi Q9CKL8. Positions 38-827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272843.PM1594.

Proteomic databases

PRIDEi Q9CKL8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK03678 ; AAK03678 ; PM1594 .
GeneIDi 1244941.
KEGGi pmu:PM1594.
PATRICi 22872479. VBIPasMul88067_1613.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi AGKMHDS.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci PMUL272843:GC8W-1656-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiNAPA_PASMU
AccessioniPrimary (citable) accession number: Q9CKL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3