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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi52Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi80Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:PM1594
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
Proteomesi
  • UP000000809 Componenti: Chromosome

Subcellular locationi

Q9CKL8:
  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Tat-type signalUniRule annotationAdd BLAST32
ChainiPRO_000004599233 – 828Periplasmic nitrate reductaseAdd BLAST796

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM1594.

Structurei

3D structure databases

ProteinModelPortaliQ9CKL8.
SMRiQ9CKL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 944Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiTTDEVWP.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
PANTHERiPTHR11615:SF123. PTHR11615:SF123. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CKL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSRRDFMK TNAAVAAAAV AGLAIPVKNV EASPDKIIKW DKAPCRFCGT
60 70 80 90 100
GCSVLVGTQN GRVVASQGDP DADVNRGLNC IKGYFLPKIM YGKDRLTSPM
110 120 130 140 150
LRMTNGKFDK HGEFTPVSWD QAFTIMAEKF KKALKEKGPN GAGMFTSGQS
160 170 180 190 200
TIFEGIAKSK LFKAGLRSNN IDPNARHCMA SAAVAFMRTF GMDEPMGCYD
210 220 230 240 250
DIEKADAFVL WGSNMAEMHP ILWSRISDRR LSHPDVKVAV LSTFEHRSFE
260 270 280 290 300
LADLGVIFTP QSDLAIMNYI ANYLIQHDAI DHDFIQKHTK FKRGETDIGY
310 320 330 340 350
GLRETHELEK AAKNVKTAGK MHDSDFEEYK KLVAPYTLEK AHEISGVPKE
360 370 380 390 400
QLEALAKMYA DPKLNIVSFW TMGFNQHTRG VWANHLIYNI HLLTGKISKP
410 420 430 440 450
GCGPFSLTGQ PSACGTAREV GTFAHRLPAD LVVTNPKHVE KAEKLWKLPK
460 470 480 490 500
GVIQTQVGYH AVAQDRALKD KKMNVLWQMC TNNMQGGPNI NQERFPGWRD
510 520 530 540 550
EENFVIVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER RTQFWYQQVK
560 570 580 590 600
APGESKSDVW QLVEFSKYFT TDEMWPAEVL AANPEYKGKT LYEVLFRNGQ
610 620 630 640 650
VDKFQVPTDK PGYMNDEAEH FGFYLQKGLF EEYAVFGRGH GHDLADFETY
660 670 680 690 700
HKARGLRWPV VDGKETLWRY REGYDPYVKE GEGVAFYGYP DKKAIILAVP
710 720 730 740 750
YEPPAEAPNA EYDLWLTTGR VLEHWHTGTM TRRVPELHRS FPNNLVWMHP
760 770 780 790 800
LDAKKRGLRH GDKVKISSRR GEMISHLDTR GRNKVPQGLV YTTFFDAGQL
810 820
ANYLTLDATD PISKETDFKK CAVKVEKA
Length:828
Mass (Da):93,356
Last modified:June 1, 2001 - v1
Checksum:i313637544961A2BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03678.1.
RefSeqiWP_005724436.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK03678; AAK03678; PM1594.
GeneIDi29389596.
KEGGipmu:PM1594.

Similar proteinsi

Entry informationi

Entry nameiNAPA_PASMU
AccessioniPrimary (citable) accession number: Q9CKL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 2001
Last modified: October 25, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families