Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative 4-hydroxythreonine-4-phosphate dehydrogenase

Gene

PM1650

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).By similarity

Miscellaneous

The active site is located at the dimer interface.By similarity

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.By similarity

Cofactori

Zn2+By similarity, Mg2+By similarity, Co2+By similarityNote: Binds 1 divalent metal cation per subunit. Can use ions such as Zn2+, Mg2+ or Co2+.By similarity

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Phosphoserine aminotransferase (serC)
  4. Putative 4-hydroxythreonine-4-phosphate dehydrogenase (PM1650)
  5. no protein annotated in this organism
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi172Divalent metal cation; shared with dimeric partnerBy similarity1
Metal bindingi216Divalent metal cation; shared with dimeric partnerBy similarity1
Metal bindingi271Divalent metal cation; shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandCobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxythreonine-4-phosphate dehydrogenaseBy similarity (EC:1.1.1.262By similarity)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseBy similarity
Gene namesi
Ordered Locus Names:PM1650
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
Proteomesi
  • UP000000809 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001888141 – 337Putative 4-hydroxythreonine-4-phosphate dehydrogenaseAdd BLAST337

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272843.PM1650.

Structurei

3D structure databases

ProteinModelPortaliQ9CKG8.
SMRiQ9CKG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.Curated

Phylogenomic databases

eggNOGiENOG4105CEZ. Bacteria.
COG1995. LUCA.
HOGENOMiHOG000221593.
KOiK00097.
OMAiCGREEID.

Family and domain databases

InterProiView protein in InterPro
IPR005255. PdxA.
PANTHERiPTHR30004. PTHR30004. 1 hit.
PfamiView protein in Pfam
PF04166. PdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CKG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSTVALTL GDPAGIGPEL VAKLLAKQNI REKANIVLVA DKDELEKGME
60 70 80 90 100
IAKAQFVYEE VSFHQLGQYE FKTGVPVLIS HKSSHPKPFE YGKVTEQSGV
110 120 130 140 150
YILETLKVAL NLAKMGYVQA ICFAPLNKQA MHKGGLRYRD ELHWFAEQTD
160 170 180 190 200
FNEFVCELNV VDDIWAARVT SHIPFKDIVP NLSIKGVFDC IHLLYRSLVQ
210 220 230 240 250
AGVENPKIAV QALNPHGGEG GVFGDEEMTI IQPGMEQARK AGIDVYGPFP
260 270 280 290 300
GDTTMREVER LKINGVVSMY HDQFSTALKI LGFERGVTVQ GGIPIPITTA
310 320 330
NHGTAFDLHG KNIAIPTAFE AAFNIAVRMG QGVLNQK
Length:337
Mass (Da):37,033
Last modified:June 1, 2001 - v1
Checksum:i7D0955556B98D657
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03734.1.
RefSeqiWP_005718533.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK03734; AAK03734; PM1650.
GeneIDi29389610.
KEGGipmu:PM1650.
PATRICi22872591. VBIPasMul88067_1669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03734.1.
RefSeqiWP_005718533.1. NC_002663.1.

3D structure databases

ProteinModelPortaliQ9CKG8.
SMRiQ9CKG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272843.PM1650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK03734; AAK03734; PM1650.
GeneIDi29389610.
KEGGipmu:PM1650.
PATRICi22872591. VBIPasMul88067_1669.

Phylogenomic databases

eggNOGiENOG4105CEZ. Bacteria.
COG1995. LUCA.
HOGENOMiHOG000221593.
KOiK00097.
OMAiCGREEID.

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.

Family and domain databases

InterProiView protein in InterPro
IPR005255. PdxA.
PANTHERiPTHR30004. PTHR30004. 1 hit.
PfamiView protein in Pfam
PF04166. PdxA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPDXAL_PASMU
AccessioniPrimary (citable) accession number: Q9CKG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.