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Q9CKF7

- SYI_PASMU

UniProt

Q9CKF7 - SYI_PASMU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei562 – 5621Aminoacyl-adenylateUniRule annotation
Binding sitei606 – 6061ATPUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-1725-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PM1662
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligasePRO_0000098434Add
BLAST

Proteomic databases

PRIDEiQ9CKF7.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM1662.

Structurei

3D structure databases

ProteinModelPortaliQ9CKF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi603 – 6075"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CKF7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVDYKNTLN LPETGFPMRG DLAKREPNML KSWYEKDLYQ KIRQASKGKK
60 70 80 90 100
SFILHDGPPY ANGTIHIGHA VNKILKDIIV KSKTALGYDS PYIPGWDCHG
110 120 130 140 150
LPIELKVEGL VGKPNQNISA AQFREACRQY AAEQVEGQKK DFIRLGVLGD
160 170 180 190 200
WDNPYLTMNY HTEANIIRAF GKAVENGHLY KGSKPVHWCL DCASSLAEAE
210 220 230 240 250
VEYEDKVSPS IYVRFSAVDS DAVLAKFNAT DKGTGNISAV IWTTTPWTIP
260 270 280 290 300
SNRAIAIHEN LDYQLVQFND ERVILAKDLV EEVAKAAGVE QVVILGESKG
310 320 330 340 350
KDLEWLRFQH PFYDFSVPFI LGDHVTTDGG TGLVHTAPDH GHDDYIIAQK
360 370 380 390 400
NGIEMAGLIG NDGLFKADVP FFAGKGVFES NDLVVAKLQE VGAMLKFSKI
410 420 430 440 450
KHSYPHCWRH KTPIIFRATP QWFIGMEKQG LRQQALSEIK KVRWIPDWGQ
460 470 480 490 500
ARIEKMVENR PDWCISRQRT WGVPVALFIH KETEELHPRT VELVEEVAKR
510 520 530 540 550
VEQKGIQAWW DLDTAELLGA DADNYIKVPD TLDVWFDSGS TYYSVVKDRP
560 570 580 590 600
EFNGQEADMY LEGSDQHRGW FMSSLMLSTA TDNKAPYKQV LTHGFTVDGQ
610 620 630 640 650
GRKMSKSIGN IVTPQEVMDK FGGDILRLWV ASTDYTGEIS VSDEILKRAA
660 670 680 690 700
DAYRRIRNTA RFLLANLNGF DPKRDLVKPE EMMVLDRWAV DCAYQAQNEI
710 720 730 740 750
KDAYDNYQFH AVIQRLMKFC SIEMGSFYLD IIKDRQYTTK ADSLARRSCQ
760 770 780 790 800
TALWHIAEAL VRWIAPVLSF TADEIWQYIP GERGEFVFTE EFYNGLFALD
810 820 830 840 850
ANEQMNDAYW QQVITLRNEV NRVLEQARND KIIGAALEAE LTIYANDTYA
860 870 880 890 900
PLLAKLQNEL RFVLLTSKAE VKPLADADVA EGEVKGFAVK VVRSANHKCP
910 920 930
RCWHYSDSKD AESLCSRCDE NVNGQGEVRQ FA
Length:932
Mass (Da):105,639
Last modified:June 1, 2001 - v1
Checksum:iC05395EC5DDC64DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03746.1.
RefSeqiNP_246601.1. NC_002663.1.
WP_005757850.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK03746; AAK03746; PM1662.
GeneIDi1245009.
KEGGipmu:PM1662.
PATRICi22872619. VBIPasMul88067_1682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03746.1 .
RefSeqi NP_246601.1. NC_002663.1.
WP_005757850.1. NC_002663.1.

3D structure databases

ProteinModelPortali Q9CKF7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272843.PM1662.

Proteomic databases

PRIDEi Q9CKF7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK03746 ; AAK03746 ; PM1662 .
GeneIDi 1245009.
KEGGi pmu:PM1662.
PATRICi 22872619. VBIPasMul88067_1682.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMUL272843:GC8W-1725-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiSYI_PASMU
AccessioniPrimary (citable) accession number: Q9CKF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3