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Q9CKF7

- SYI_PASMU

UniProt

Q9CKF7 - SYI_PASMU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei562 – 5621Aminoacyl-adenylateUniRule annotation
    Binding sitei606 – 6061ATPUniRule annotation
    Metal bindingi899 – 8991ZincUniRule annotation
    Metal bindingi902 – 9021ZincUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPMUL272843:GC8W-1725-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PM1662
    OrganismiPasteurella multocida (strain Pm70)
    Taxonomic identifieri272843 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
    ProteomesiUP000000809: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 932932Isoleucine--tRNA ligasePRO_0000098434Add
    BLAST

    Proteomic databases

    PRIDEiQ9CKF7.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272843.PM1662.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CKF7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi603 – 6075"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CKF7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVDYKNTLN LPETGFPMRG DLAKREPNML KSWYEKDLYQ KIRQASKGKK    50
    SFILHDGPPY ANGTIHIGHA VNKILKDIIV KSKTALGYDS PYIPGWDCHG 100
    LPIELKVEGL VGKPNQNISA AQFREACRQY AAEQVEGQKK DFIRLGVLGD 150
    WDNPYLTMNY HTEANIIRAF GKAVENGHLY KGSKPVHWCL DCASSLAEAE 200
    VEYEDKVSPS IYVRFSAVDS DAVLAKFNAT DKGTGNISAV IWTTTPWTIP 250
    SNRAIAIHEN LDYQLVQFND ERVILAKDLV EEVAKAAGVE QVVILGESKG 300
    KDLEWLRFQH PFYDFSVPFI LGDHVTTDGG TGLVHTAPDH GHDDYIIAQK 350
    NGIEMAGLIG NDGLFKADVP FFAGKGVFES NDLVVAKLQE VGAMLKFSKI 400
    KHSYPHCWRH KTPIIFRATP QWFIGMEKQG LRQQALSEIK KVRWIPDWGQ 450
    ARIEKMVENR PDWCISRQRT WGVPVALFIH KETEELHPRT VELVEEVAKR 500
    VEQKGIQAWW DLDTAELLGA DADNYIKVPD TLDVWFDSGS TYYSVVKDRP 550
    EFNGQEADMY LEGSDQHRGW FMSSLMLSTA TDNKAPYKQV LTHGFTVDGQ 600
    GRKMSKSIGN IVTPQEVMDK FGGDILRLWV ASTDYTGEIS VSDEILKRAA 650
    DAYRRIRNTA RFLLANLNGF DPKRDLVKPE EMMVLDRWAV DCAYQAQNEI 700
    KDAYDNYQFH AVIQRLMKFC SIEMGSFYLD IIKDRQYTTK ADSLARRSCQ 750
    TALWHIAEAL VRWIAPVLSF TADEIWQYIP GERGEFVFTE EFYNGLFALD 800
    ANEQMNDAYW QQVITLRNEV NRVLEQARND KIIGAALEAE LTIYANDTYA 850
    PLLAKLQNEL RFVLLTSKAE VKPLADADVA EGEVKGFAVK VVRSANHKCP 900
    RCWHYSDSKD AESLCSRCDE NVNGQGEVRQ FA 932
    Length:932
    Mass (Da):105,639
    Last modified:June 1, 2001 - v1
    Checksum:iC05395EC5DDC64DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004439 Genomic DNA. Translation: AAK03746.1.
    RefSeqiNP_246601.1. NC_002663.1.
    WP_005757850.1. NC_002663.1.

    Genome annotation databases

    EnsemblBacteriaiAAK03746; AAK03746; PM1662.
    GeneIDi1245009.
    KEGGipmu:PM1662.
    PATRICi22872619. VBIPasMul88067_1682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004439 Genomic DNA. Translation: AAK03746.1 .
    RefSeqi NP_246601.1. NC_002663.1.
    WP_005757850.1. NC_002663.1.

    3D structure databases

    ProteinModelPortali Q9CKF7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272843.PM1662.

    Proteomic databases

    PRIDEi Q9CKF7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK03746 ; AAK03746 ; PM1662 .
    GeneIDi 1245009.
    KEGGi pmu:PM1662.
    PATRICi 22872619. VBIPasMul88067_1682.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PMUL272843:GC8W-1725-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pm70.

    Entry informationi

    Entry nameiSYI_PASMU
    AccessioniPrimary (citable) accession number: Q9CKF7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3