Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei562Aminoacyl-adenylateUniRule annotation1
Binding sitei606ATPUniRule annotation1
Metal bindingi899ZincUniRule annotation1
Metal bindingi902ZincUniRule annotation1
Metal bindingi915ZincUniRule annotation1
Metal bindingi918ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMUL272843:G1FZ8-1746-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PM1662
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
Proteomesi
  • UP000000809 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984341 – 932Isoleucine--tRNA ligaseAdd BLAST932

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272843.PM1662

Structurei

3D structure databases

ProteinModelPortaliQ9CKF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi603 – 607"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CKF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVDYKNTLN LPETGFPMRG DLAKREPNML KSWYEKDLYQ KIRQASKGKK
60 70 80 90 100
SFILHDGPPY ANGTIHIGHA VNKILKDIIV KSKTALGYDS PYIPGWDCHG
110 120 130 140 150
LPIELKVEGL VGKPNQNISA AQFREACRQY AAEQVEGQKK DFIRLGVLGD
160 170 180 190 200
WDNPYLTMNY HTEANIIRAF GKAVENGHLY KGSKPVHWCL DCASSLAEAE
210 220 230 240 250
VEYEDKVSPS IYVRFSAVDS DAVLAKFNAT DKGTGNISAV IWTTTPWTIP
260 270 280 290 300
SNRAIAIHEN LDYQLVQFND ERVILAKDLV EEVAKAAGVE QVVILGESKG
310 320 330 340 350
KDLEWLRFQH PFYDFSVPFI LGDHVTTDGG TGLVHTAPDH GHDDYIIAQK
360 370 380 390 400
NGIEMAGLIG NDGLFKADVP FFAGKGVFES NDLVVAKLQE VGAMLKFSKI
410 420 430 440 450
KHSYPHCWRH KTPIIFRATP QWFIGMEKQG LRQQALSEIK KVRWIPDWGQ
460 470 480 490 500
ARIEKMVENR PDWCISRQRT WGVPVALFIH KETEELHPRT VELVEEVAKR
510 520 530 540 550
VEQKGIQAWW DLDTAELLGA DADNYIKVPD TLDVWFDSGS TYYSVVKDRP
560 570 580 590 600
EFNGQEADMY LEGSDQHRGW FMSSLMLSTA TDNKAPYKQV LTHGFTVDGQ
610 620 630 640 650
GRKMSKSIGN IVTPQEVMDK FGGDILRLWV ASTDYTGEIS VSDEILKRAA
660 670 680 690 700
DAYRRIRNTA RFLLANLNGF DPKRDLVKPE EMMVLDRWAV DCAYQAQNEI
710 720 730 740 750
KDAYDNYQFH AVIQRLMKFC SIEMGSFYLD IIKDRQYTTK ADSLARRSCQ
760 770 780 790 800
TALWHIAEAL VRWIAPVLSF TADEIWQYIP GERGEFVFTE EFYNGLFALD
810 820 830 840 850
ANEQMNDAYW QQVITLRNEV NRVLEQARND KIIGAALEAE LTIYANDTYA
860 870 880 890 900
PLLAKLQNEL RFVLLTSKAE VKPLADADVA EGEVKGFAVK VVRSANHKCP
910 920 930
RCWHYSDSKD AESLCSRCDE NVNGQGEVRQ FA
Length:932
Mass (Da):105,639
Last modified:June 1, 2001 - v1
Checksum:iC05395EC5DDC64DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA Translation: AAK03746.1
RefSeqiWP_005757850.1, NC_002663.1

Genome annotation databases

EnsemblBacteriaiAAK03746; AAK03746; PM1662
KEGGipmu:PM1662

Similar proteinsi

Entry informationi

Entry nameiSYI_PASMU
AccessioniPrimary (citable) accession number: Q9CKF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health