Q9CKF5 (NTPA_PASMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

PM1666

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178205
Regions
	Region	9 – 14	6	Substrate binding By similarity
	Region	70 – 71	2	Substrate binding By similarity
Sites
	Metal binding	41	1	Magnesium or manganese By similarity
	Metal binding	70	1	Magnesium or manganese By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CKF5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 4E2AC3F04CB77F7E	FASTA	202	21,615
	10 20 30 40 50 60 MKQKIVLATG NLGKVKEMSD VLADFGFEVI AQTELNIESP EETGLTFVEN ALLKARYASK 70 80 90 100 110 120 MSGLPAIADD SGLVVPALGG APGLYSARYA GVDGPDADAK NRAKLLHVLH HIAPTHRQAK 130 140 150 160 170 180 FVSCIVMLQH EHDPSPIIAE GECYGEIGFA EKGENGFGYD SLFFSPEVNC TFAELATSEK 190 200 KKISHRAKAL SVLQTKLATK GA

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK03750.1.
RefSeq	NP_246605.1. NC_002663.1.
3D structure databases
ProteinModelPortal	Q9CKF5.
SMR	Q9CKF5. Positions 3-197.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1245013.
GenomeReviews	Gene locus PM1666 in contig AE004439_GR.
KEGG	pmu:PM1666.
NMPDR	fig|272843.1.peg.1667.
PATRIC	22872627. VBIPasMul88067_1686.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	YSKRYDQ.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	PMUL272843:PM1666-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_PASMU

Accession

Primary (citable) accession number: Q9CKF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program