Reviewed, UniProtKB/Swiss-Prot Q9CK41 (TORA_PASMU)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase
      Short name=TMAO reductase
      Short name=Trimethylamine oxidase
    EC=1.7.2.3

Gene names

Name:	torA
Ordered Locus Names:	PM1793

Organism

Pasteurella multocida [Complete proteome] [HAMAP]

Taxonomic identifier

747 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	823 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions By similarity.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Tat-type signal Potential
	Chain	33 – 823	791	Trimethylamine-N-oxide reductase		PRO_0000019154

Sequences

	Sequence		Length	Mass (Da)
	Q9CK41-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: BECAC53FABEE250F	FASTA	823	93,357
	10 20 30 40 50 60 MKQSRRQFLK NMSAMAATFA MPNFLIAQNA FAQSAENVSE WKITGSHWGA IRAKVQNGKV 70 80 90 100 110 120 VDVKPFEYDQ YPTEMIKGIK DLIYSEARIR YPMVRLDWLK KRHNSNTAQR GDNRFVRVTW 130 140 150 160 170 180 DEALDLFYEE LERIQQNYGP WALHTGNVGW RSTGQFHSCG NHMIRAVGMH GNSVSTSGDY 190 200 210 220 230 240 STGAGQVILP YVLGSTEVYS QGTSWEIILK ESENIIFWAS DPVKNLQVGW NCETHEAYKY 250 260 270 280 290 300 LEQLKAKVAA KDVNVICVDP VKSKTQNYLG CDFQYINPQT DVAFMLALAH TLYVENLYDK 310 320 330 340 350 360 KFIEMYTVGF EKFLPYLLGE SEDKVVKDAE WAAKICAIQA EDIRQFARML AGKRTQLIFG 370 380 390 400 410 420 WAIQRQQHGE QPYWMGTVLA AMLGQIGLAG GGISYAHHYS SIGIPSSGAA MPGAFPLNLD 430 440 450 460 470 480 EGQKPKYDNK NYNGYSAVIP CARITDSLLQ PGETIDHNGQ KITYAPYKMA IFTGCNHWHR 490 500 510 520 530 540 HSERNKMKQA FQRLETIVSI NYSWTATCRF SDIVLPACTP FERNDIDAYG SYSNRGVIAM 550 560 570 580 590 600 QKLVDPLYDS RSDFEIFKDL CRRFGKEKEY CRNMDEMEWV KHLYEACRQE NQGKFDMPPF 610 620 630 640 650 660 AEFWQKGYVL FPEGEPWVRH ADFREDPELH ALGTPSGFIE IFSNKIASYG YADCKGHPMW 670 680 690 700 710 720 FEKAERSHGG PKSDKYPFWL QSAHPDKRLH SQLCESKALR ETYAIQDREP LFINPEDAKR 730 740 750 760 770 780 LGIVHGDLVR VYNDRGQAIV GAHVSDNFPQ GVLRLQEGAW YSPLDEKVGS IDTYGDPNTM 790 800 810 820 SLDIGSSSLA QAVSANTCLV NIEKFVGQAP AVTGFHGPHE VAL

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK03877.1.
RefSeq	NP_246732.1.
3D structure databases
SMR	Q9CK41. Positions 37-823.
ModBase	Search...
Genome annotation databases
GeneID	1245140.
GenomeReviews	Gene locus PM1793 in contig AE004439_GR.
KEGG	pmu:PM1793.
NMPDR	fig|272843.1.peg.1794.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304064.
OMA	NAETHES.
Enzyme and pathway databases
BRENDA	1.7.2.3. 258935.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006658. BisC. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006311. TAT_signal. IPR011887. TorA. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR00509. bisC_fam. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. TIGR02164. torA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORA_PASMU

Accession

Primary (citable) accession number: Q9CK41

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)