Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate 5-kinase

Gene

proB

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATPUniRule annotation1
Binding sitei50SubstrateUniRule annotation1
Binding sitei137SubstrateUniRule annotation1
Binding sitei149Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi169 – 170ATPUniRule annotation2
Nucleotide bindingi211 – 217ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:PM1896
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
Proteomesi
  • UP000000809 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097021 – 367Glutamate 5-kinaseAdd BLAST367

Interactioni

Protein-protein interaction databases

STRINGi272843.PM1896.

Structurei

3D structure databases

ProteinModelPortaliQ9CJU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini275 – 353PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246369.
KOiK00931.
OMAiVIHRDHW.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CJU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIDKTLVVK FGTSTLTQGS AKLNLPHMMD IVRQLAQLHQ AGFRLVIVTS
60 70 80 90 100
GAIAAGRHYL NHPQLPPTIA SKQLLAAVGQ SQLIQTWEKL FAIYDIHIGQ
110 120 130 140 150
ILLTRADIED RERFLNARDT LRALLDNQII PVINENDAVA TSEIKVGDND
160 170 180 190 200
NLSALVAILV QAEQLYLLTD QQGLFDRDPR KHPDAKLIAD VDKITDHIRA
210 220 230 240 250
IAGGSGTSLG TGGMSTKISA ADVATRSGIE TIIAPGNRPN VIVDLAYGQA
260 270 280 290 300
IGTKFSVQAD RLESRKQWLY AAPSAGIITI DAGAENAMLM QHKSLLPAGI
310 320 330 340 350
VNVEGRFSRG EVVKIRTQQG KDVALGMPRY NSDALHLIQG KHSQDIEQVL
360
GYEYGAVAVH RDDMIVL
Length:367
Mass (Da):39,895
Last modified:September 26, 2001 - v2
Checksum:iE62DA632EC78F6E5
GO

Sequence cautioni

The sequence AAK03980 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03980.1. Different initiation.
RefSeqiWP_005724984.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK03980; AAK03980; PM1896.
GeneIDi1245243.
KEGGipmu:PM1896.
PATRICi22873115. VBIPasMul88067_1918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK03980.1. Different initiation.
RefSeqiWP_005724984.1. NC_002663.1.

3D structure databases

ProteinModelPortaliQ9CJU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272843.PM1896.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK03980; AAK03980; PM1896.
GeneIDi1245243.
KEGGipmu:PM1896.
PATRICi22873115. VBIPasMul88067_1918.

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246369.
KOiK00931.
OMAiVIHRDHW.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_PASMU
AccessioniPrimary (citable) accession number: Q9CJU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: February 15, 2017
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.