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Q9CJD1 (SYW_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:LL0066
ORF Names:L0358
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136640

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140
Motif202 – 2065"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9CJD1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: CDD0EF2AF8EF8D87

FASTA34138,051
        10         20         30         40         50         60 
MTKPTILTGD RPTGKLHIGH YVGSLQNRVK MQNEDKYNMF VFLADQQALT DHAKEPELIR 

        70         80         90        100        110        120 
QSVGDVALDY LAAGLDPEKS TIFIQSQIPE LAELSMYYMN LVSLARLERN PTVKTEIAQK 

       130        140        150        160        170        180 
AFGESIPAGF LVYPVSQAAD ITAFKATHVP VGNDQKPMIE QTREIVRSFN NAYHTDVLVE 

       190        200        210        220        230        240 
PEGIYPENEA AGRLPGLDGN AKMSKSLGNG IFLADDMDTL KKKVMSMYTD PDHIKVEDPG 

       250        260        270        280        290        300 
KIEGNMVFHY LDVFGKAEDA EQISEMKAQY QAGGLGDVKT KRFLLDVLDR ELSPIRERRI 

       310        320        330        340 
EFAQNMDYVY DMLKAGSLKA QAVASQTLDE VKSAMGINYF K 

« Hide

References

[1]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005176 Genomic DNA. Translation: AAK04164.1.
PIRB86633.
RefSeqNP_266222.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ9CJD1.
SMRQ9CJD1. Positions 2-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L0358.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK04164; AAK04164; L0358.
GeneID1113669.
KEGGlla:L0358.
PATRIC22292266. VBILacLac136773_0071.

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059941.
KOK01867.
OMAYGYGHAK.
OrthoDBEOG686NJQ.
ProtClustDBPRK12282.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-79-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_LACLA
AccessionPrimary (citable) accession number: Q9CJD1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries