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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Proton acceptorUniRule annotation1
Binding sitei151SubstrateUniRule annotation1
Binding sitei194SubstrateUniRule annotation1
Binding sitei221Substrate; via amide nitrogenUniRule annotation1
Active sitei271NucleophileUniRule annotation1
Metal bindingi309ZincUniRule annotation1
Metal bindingi311ZincUniRule annotation1
Metal bindingi314ZincUniRule annotation1
Metal bindingi340Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processQueuosine biosynthesis, tRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciLLAC272623:G1FZA-174-MONOMER
UniPathwayiUPA00392

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:LL0152
ORF Names:L0361
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001354861 – 382Queuine tRNA-ribosyltransferaseAdd BLAST382

Proteomic databases

PaxDbiQ9CJ54
PRIDEiQ9CJ54

Interactioni

Subunit structurei

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.UniRule annotation

Protein-protein interaction databases

STRINGi272623.L0361

Structurei

3D structure databases

ProteinModelPortaliQ9CJ54
SMRiQ9CJ54
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 100Substrate bindingUniRule annotation5
Regioni252 – 258RNA bindingUniRule annotation7
Regioni276 – 280RNA binding; important for wobble base 34 recognitionUniRule annotation5

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6U Bacteria
COG0343 LUCA
HOGENOMiHOG000223473
KOiK00773
OMAiTYHLFLR

Family and domain databases

Gene3Di3.20.20.105, 1 hit
HAMAPiMF_00168 Q_tRNA_Tgt, 1 hit
InterProiView protein in InterPro
IPR004803 TGT
IPR036511 TGT-like_sf
IPR002616 tRNA_ribo_trans-like
PfamiView protein in Pfam
PF01702 TGT, 1 hit
SUPFAMiSSF51713 SSF51713, 1 hit
TIGRFAMsiTIGR00430 Q_tRNA_tgt, 1 hit
TIGR00449 tgt_general, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CJ54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEHAIKYRL IKKEKHTGAR LGEIITPHGT FPTPMFMPVG TQATVKTMSP
60 70 80 90 100
EELKTLGSGI ILSNTYHLWL RPGDELVAEA GGLHKFMNWD QPILTDSGGF
110 120 130 140 150
QVYSLVQNKK NITEEGVKFK SHLDGRELFL NPEKAISIQN NLGSDIMMSF
160 170 180 190 200
DECPPFYQPY DYVKASVERT SRWAERGLNA HRRPNDQGLF GIVQGAGFED
210 220 230 240 250
LRRQSARDLT SMDFAGYSIG GLAVGESHKE MNAVLDFTTP MLPEDKPRYL
260 270 280 290 300
MGVGAPDSLI DGVIRGVDMF DCVLPTRIAR NGTLMTHFGR VNIRNAKYEH
310 320 330 340 350
DFTPLDPMCD CYTCTNYTRA YLRHLIKADE TFGLRLCSYH NLHFLVNLMK
360 370 380
DVRGAIMDDN LLEFREDFCE RYGYNQPNAK DF
Length:382
Mass (Da):43,342
Last modified:April 27, 2001 - v1
Checksum:iFDD3F19AFC90584D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA Translation: AAK04250.1
PIRiH86643
RefSeqiNP_266308.1, NC_002662.1
WP_003129711.1, NC_002662.1

Genome annotation databases

EnsemblBacteriaiAAK04250; AAK04250; L0361
GeneIDi1113760
KEGGilla:L0361
PATRICifig|272623.7.peg.170

Similar proteinsi

Entry informationi

Entry nameiTGT_LACLA
AccessioniPrimary (citable) accession number: Q9CJ54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health