Q9CJ43 (GATA_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	LL0163
ORF Names:	L0473

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP MF_00120		PRO_0000105168
Sites
	Active site	77	1	Charge relay system By similarity
	Active site	152	1	Charge relay system By similarity
	Active site	176	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9CJ43 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: 52FC956059F05EF9	FASTA	486	52,071
	10 20 30 40 50 60 MSYNNKSIKE LHELLVNKEI SALELTKATL SDISAREPQI DAFLKITEEK ALQDAAAIDA 70 80 90 100 110 120 RGINPDVVTD GISIAVKDNI VTEGIETTAA SKILGGWIPP YNATVANKLS ESGLITIGKL 130 140 150 160 170 180 NMDEFAMGGS GENSSVKPTK NAWDQTKVPG GSSSGSAASV ASGQVRLSLG SDTGGSIRQP 190 200 210 220 230 240 AAFNGIVGLK PTYGRVSRFG LIAFASSLDQ IGPLAPTVEE NAQLLNVISG FDKNDSTSSN 250 260 270 280 290 300 VAVPDFTSKI GQDIKGMKIA LPKEYFGEGI DEKVKEQILA AAKHLEKLGA IVEEVSLPHS 310 320 330 340 350 360 KYGVAVYYII ASSEASSNLQ RFDGIRYGYR AQDIKNLEDL YVKSRSEGFG PEVQRRIMLG 370 380 390 400 410 420 TFSLSAGSYD KHFKKAGQVR TLIINDFAKV FEKYDLILGP TTPTPAWDLG ARVDDPLSMY 430 440 450 460 470 480 LADLLTIPVN LAGLPGISIP AGFADGLPVG MQLIGKRYDE ETIYKVAAAF EATTDFHKKQ PIIFGK

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04261.1.
PIR	C86645.
RefSeq	NP_266319.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CJ43.
SMR	Q9CJ43. Positions 1-482.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1113771.
GenomeReviews	Gene locus LL0163 in contig AE005176_GR.
KEGG	lla:L0473.
NMPDR	fig|272623.1.peg.170.
PATRIC	22292492. VBILacLac136773_0181.
Phylogenomic databases
HOGENOM	HBG481888.
OMA	TESSCYG.
ProtClustDB	PRK00012.
Enzyme and pathway databases
BioCyc	LLAC272623:L0473-MONOMER.
Family and domain databases
HAMAP	MF_00120. GatA. [Tree]
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
Gene3D	G3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit.
KO	K02433.
PANTHER	PTHR11895. Amidase. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. GatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_LACLA

Accession

Primary (citable) accession number: Q9CJ43

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program