Q9CJ41 (GATB_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	LL0165
ORF Names:	L0474

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00121
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.
Sequence caution	The sequence AAK04263.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP MF_00121		PRO_0000148797

Sequences

	Sequence		Length	Mass (Da)
	Q9CJ41 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: 8CB892E7EF0F6C8E	FASTA	477	53,425
	10 20 30 40 50 60 MNFETVIGLE VHVELSTNSK IFSPASTKFG GDPNTNTNVI DWSLPGVLPV MNKGVIDSGI 70 80 90 100 110 120 KAALALNMDI HKSMHFDRKN YFYPDNPKAY QISQFDEPIG YNGSIEIELE DGHKATIRIE 130 140 150 160 170 180 RAHLEEDAGK NTHGTDGYSY VDLNRQGVPL IEIVSEADMR TPEEAYAYLT ALKEAILYTG 190 200 210 220 230 240 ISDVKMEEGS MRCDANVSLR PYGQEAFGVK TEVKNMNSFS NVKKALDFEV DRQAKILRAG 250 260 270 280 290 300 GEIRQETRRF NDKTGETILM RVKEGASDYR YFPEPDVPRF EISEEWVEQM RESLPMTAKA 310 320 330 340 350 360 RRAHYINDLA LSDYDARQLT ATKEVSDFFD QAIKFDTDPK LVSNWLQGEV AQYLNSEKKE 370 380 390 400 410 420 LHEIGLTPEN LTEMIRLISD GTISSKIAKK VFIELAKNGG SAEEFVKKAG LVQISDPAVL 430 440 450 460 470 LPIIHDVFAK NEQSVADYRG GKQNAAKALV GQLMKATKGQ ANPTVAQKLL YQELDNF

References

[1]

"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04263.1. Different initiation.
PIR	E86645.
RefSeq	NP_266321.2. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9CJ41.
SMR	Q9CJ41. Positions 2-413.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1113773.
GenomeReviews	Gene locus LL0165 in contig AE005176_GR.
KEGG	lla:L0474.
NMPDR	fig|272623.1.peg.172.
PATRIC	22292496. VBILacLac136773_0183.
Phylogenomic databases
HOGENOM	HBG395951.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Enzyme and pathway databases
BioCyc	LLAC272623:L0474-MONOMER.
Family and domain databases
HAMAP	MF_00121. GatB. [Tree]
InterPro	IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR004413. Gln-tRNA_amidoTrfase_bsu. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
KO	K02434.
PANTHER	PTHR11659. GatB. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. GatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_LACLA

Accession

Primary (citable) accession number: Q9CJ41

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 25, 2002
Last modified:	January 25, 2012
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program