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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.By similarity

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciLLAC272623:G1FZA-199-MONOMER
UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:dut
Ordered Locus Names:LL0176
ORF Names:L181168
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001828721 – 150Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST150

Proteomic databases

PaxDbiQ9CJ30
PRIDEiQ9CJ30

Interactioni

Protein-protein interaction databases

STRINGi272623.L181168

Structurei

3D structure databases

ProteinModelPortaliQ9CJ30
SMRiQ9CJ30
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 69Substrate bindingBy similarity3
Regioni84 – 86Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

eggNOGiENOG4107Z0P Bacteria
COG0756 LUCA
HOGENOMiHOG000028967
KOiK01520
OMAiHIFWTDI

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CJ30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIRGFEVVT KYKNAGINIP KRSTEHSAGY DIEAAETVSF APGEIKLIPT
60 70 80 90 100
GLKAYMQAGE VLYMYDRSSN PRKKGLVLIN SVGVIDKDYY NNPDNEGHMF
110 120 130 140 150
MQMRNFTDEE VVIEKGERVV QGVFMPFLVA DGDENQEKEE RTGGFGSTGA
Length:150
Mass (Da):16,722
Last modified:April 27, 2001 - v1
Checksum:i6702DBCF10942516
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA Translation: AAK04274.1
PIRiH86646
RefSeqiNP_266332.1, NC_002662.1
WP_010905176.1, NC_002662.1

Genome annotation databases

EnsemblBacteriaiAAK04274; AAK04274; L181168
GeneIDi1113785
KEGGilla:L181168
PATRICifig|272623.7.peg.198

Similar proteinsi

Entry informationi

Entry nameiDUT_LACLA
AccessioniPrimary (citable) accession number: Q9CJ30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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