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Protein

PTS-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL

Gene

dhaL

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ADP-binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone.1 Publication

Pathwayi: glycerol degradation

This protein is involved in the pathway glycerol degradation, which is part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the pathway glycerol degradation and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115ADP; via carbonyl oxygen1 Publication1
Binding sitei161ADP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 40ADP1 Publication7
Nucleotide bindingi78 – 79ADP1 Publication2
Nucleotide bindingi174 – 176ADP1 Publication3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00616.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS-dependent dihydroxyacetone kinase, ADP-binding subunit DhaL (EC:2.7.-.-)
Gene namesi
Name:dhaL
Ordered Locus Names:LL0249
ORF Names:L46694
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114R → A: Reduces activity 3-fold. 1 Publication1
Mutagenesisi114R → E: Reduces activity 100-fold. 1 Publication1
Mutagenesisi161R → A: Loss of activity. 1 Publication1
Mutagenesisi164Y → A: Reduces activity about 20-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002705351 – 192PTS-dependent dihydroxyacetone kinase, ADP-binding subunit DhaLAdd BLAST192

Proteomic databases

PaxDbiQ9CIV7.

Expressioni

Inductioni

Induced by dihydroxyacetone via the DhaQ-DhaS complex.

Interactioni

Subunit structurei

The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.1 Publication

Protein-protein interaction databases

STRINGi272623.L46694.

Structurei

Secondary structure

1192
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 20Combined sources17
Helixi22 – 28Combined sources7
Turni29 – 32Combined sources4
Helixi37 – 54Combined sources18
Helixi60 – 74Combined sources15
Helixi79 – 94Combined sources16
Helixi100 – 115Combined sources16
Beta strandi121 – 123Combined sources3
Helixi125 – 136Combined sources12
Helixi142 – 151Combined sources10
Helixi152 – 154Combined sources3
Helixi161 – 169Combined sources9
Helixi175 – 191Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CR3X-ray2.10A/B2-192[»]
ProteinModelPortaliQ9CIV7.
SMRiQ9CIV7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9CIV7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 189DhaLPROSITE-ProRule annotationAdd BLAST185

Sequence similaritiesi

Contains 1 DhaL domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108UTZ. Bacteria.
COG2376. LUCA.
HOGENOMiHOG000226525.
KOiK05879.
OMAiMAKGFGM.

Family and domain databases

InterProiIPR012737. DhaK_L_YcgS.
IPR004007. DhaL_dom.
[Graphical view]
PfamiPF02734. Dak2. 1 hit.
[Graphical view]
SMARTiSM01120. Dak2. 1 hit.
[Graphical view]
SUPFAMiSSF101473. SSF101473. 1 hit.
TIGRFAMsiTIGR02365. dha_L_ycgS. 1 hit.
PROSITEiPS51480. DHAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CIV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTIDTTIEW LGKFNEKIQE NKAYLSELDG PIGDGDHGAN MARGMSETMK
60 70 80 90 100
ALEVSNFGNV SEIFKKVAMT LMSKVGGASG PLYGSAFLAM SKTAIETLDT
110 120 130 140 150
SELIYAGLEA IQKRGKAQVG EKTMVDIWSA FLNDLQTDSA SKDNLEKVVK
160 170 180 190
ASAGLLATKG RASYLGERSI GHIDPGTQSS AYLFETLLEV VA
Length:192
Mass (Da):20,578
Last modified:June 1, 2001 - v1
Checksum:i7609CBD5D97D1751
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK04347.1.
PIRiA86656.
RefSeqiNP_266405.1. NC_002662.1.
WP_010905236.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK04347; AAK04347; L46694.
GeneIDi1113859.
KEGGilla:L46694.
PATRICi22292678. VBILacLac136773_0274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005176 Genomic DNA. Translation: AAK04347.1.
PIRiA86656.
RefSeqiNP_266405.1. NC_002662.1.
WP_010905236.1. NC_002662.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CR3X-ray2.10A/B2-192[»]
ProteinModelPortaliQ9CIV7.
SMRiQ9CIV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L46694.

Proteomic databases

PaxDbiQ9CIV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK04347; AAK04347; L46694.
GeneIDi1113859.
KEGGilla:L46694.
PATRICi22292678. VBILacLac136773_0274.

Phylogenomic databases

eggNOGiENOG4108UTZ. Bacteria.
COG2376. LUCA.
HOGENOMiHOG000226525.
KOiK05879.
OMAiMAKGFGM.

Enzyme and pathway databases

UniPathwayiUPA00616.

Miscellaneous databases

EvolutionaryTraceiQ9CIV7.

Family and domain databases

InterProiIPR012737. DhaK_L_YcgS.
IPR004007. DhaL_dom.
[Graphical view]
PfamiPF02734. Dak2. 1 hit.
[Graphical view]
SMARTiSM01120. Dak2. 1 hit.
[Graphical view]
SUPFAMiSSF101473. SSF101473. 1 hit.
TIGRFAMsiTIGR02365. dha_L_ycgS. 1 hit.
PROSITEiPS51480. DHAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHAL_LACLA
AccessioniPrimary (citable) accession number: Q9CIV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.